Many studies have used velocity measurements, waveform analyses, and theoretical flagella models to investigate the establishment, maintenance, and function of flagella of the biflagellate green algae Chlamydomonas reinhardtii. We report the first direct measurement of Chlamydomonas flagellar swimming force. Using an optical trap ("optical tweezers") we detect a 75% decrease in swimming force between wild type (CC124) cells and mutants lacking outer flagellar dynein arms (oda1). This difference is consistent with previous estimates and validates the force measurement approach. To examine mechanisms underlying flagella organization and function, we deflagellated cells and examined force generation during flagellar regeneration. As expected, fully regenerated flagella are functionally equivalent to flagella of untreated wild type cells. However, analysis of swimming force vs. flagella length and the increase in force over regeneration time reveals intriguing patterns where increases in force do not always correspond with increases in length. These investigations of flagellar force, therefore, contribute to the understanding of Chlamydomonas motility, describe phenomena surrounding flagella regeneration, and demonstrate the advantages of the optical trapping technique in studies of cell motility.
We investigate photodetachment from negative ions in a homogeneous 1.0-T
magnetic field and a parallel electric field of approximately 10 V/cm. A
theoretical model for detachment in combined fields is presented. Calculations
show that a field of 10 V/cm or more should considerably diminish the Landau
structure in the detachment cross section. The ions are produced and stored in
a Penning ion trap and illuminated by a single-mode dye laser. We present
preliminary results for detachment from S- showing qualitative agreement with
the model. Future directions of the work are also discussed.Comment: Nine pages, five figures, minor revisions showing final publicatio
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