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Mechanical microenvironment plays a key role in the regulation of the phenotype and function of cardiac cells, which are strongly associated with the intracellular redox mechanism of cardiomyocytes. However, the relationship between the redox state of cardiomyocytes and their mechanical microenvironment remains elusive. In this work, we used polyacrylamide (PA) gels with varying stiffness (6.5−92.5 kPa) as the substrate to construct a mechanical microenvironment for cardiomyocytes. Then we employed scanning electrochemical microscopy (SECM) to in situ characterize the redox state of a single cardiomyocyte in terms of the apparent rate constant (k f ) of the regeneration rate of ferrocenecarboxylic by glutathione (GSH) released from cardiomyocyte, which is the most abundant reactant of intracellular reductive-oxidative metabolic cycles in cells and can represent the redox level of cardiomyocytes. The obtained SECM results show that the cardiomyocytes cultured on the stiffer substrates present lower k f values than those on the softer ones, that is, the more oxidative state of cardiomyocytes on the stiffer substrates compared to those on the softer ones. It proves the relationship between mechanical factors and the redox state of cardiomyocytes. This work can contribute to understanding the intracellular chemical process of cardiomyocytes during physiopathologic conditions. Besides, it also provides a new SECM method to in situ investigate the redox mechanism of cardiomyocytes at a single-cell level.

A Co II 18 L 6 hexameric metal−organic nanocapsule (MONC) has been prepared and characterized using biomimetic self-assembly as the synthetic methodology. Akin to the biological behavior of zinc-finger proteins' release, uptake, and electrophilic substitution of Zn 2+ ions, the assembly of this novel MONC has been accomplished by employing three sequential processes: assembly of the framework, metal ion insertion, and metal exchange, resulting in the formation of the Co II 18 L 6 hexameric MONC. In this work, inspired by the biological behavior of metalloproteins, rational control of multiple complex supramolecular self-assembly has been achieved.

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