Objective
Our aim was to establish a chemiluminescence method for detecting anti-transmembrane protein (p7) antibody in the serum of patients with hepatitis C virus (HCV) infection.
Methods
The p7 gene was amplified by polymerase chain reaction using the plasmid PUC-p7 containing the p7 nucleic acid sequence of the HCV 1b genotype as the template, and recombinant plasmid pGEX-KG-p7 was constructed. After p7 fusion, the protein was induced and expressed in the prokaryote, extracted, and purified; the anti-p7 antibody detection kit was prepared, and its efficacy was evaluated.
Results
The plasmid pGEX-KG-p7 was constructed correctly, and p7 fusion protein was obtained. The methodological indexes of the kit, the precision test, blank limit and detection limit, etc, met the requirements. The positive rate of serum anti-p7 antibody in 45 patients with HCV infection was 20%.
Conclusions
The kit can be used in screening diagnosis, condition monitoring, prognosis, and disease mechanism and epidemiological study of HCV infection. The p7 protein has immune response in HCV-infected patients.
Somnonetz Eine digitale Lösung zur schlafmedizinischen QualitätssicherungSeit 20 Jahren führt die DGSM in regelmäßigen Abständen eine Qualitätssicherung (QS) unter den ihr angeschlossenen Schlaflaboren durch. Dieser Prozess trägt dazu bei, die Sicherung und Verbesserung der Qualität der Patientenversorgung zu gewährleisten. Die Deutsche Gesellschaft für Schlafforschung und Schlafmedizin (DGSM) bescheinigt den akkreditierten Schlaflaboren dadurch einen Minimalstandard in Bezug auf strukturelle [2] und prozedurale [3] Qualität, wodurch sichergestellt wird, dass jedes Schlaflabor die Voraussetzungen für die dort durchgeführten Untersuchungen erfüllt.
Among HBV-infected persons, there is a group of people with hepatitis B surface antigen (HBsAg) showing persistently low levels of expression. The production of low-level HBsAg does not mean a good outcome of chronic HBV infection. Patients still have virus replication and sustained liver damage, and they have the potential to transmit the infection. This risk poses a challenge to clinical diagnosis and blood transfusion safety and is a major concern of experts. However, the mechanism behind persistent low-level HBsAg expression in serum is not completely clear, and complete virus clearance by the host is vital. In this review, we summarize the research progress on the mechanism behind low-level expression of HBsAg in patients with chronic HBV infection in recent years.
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