Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated malignancy with a complex tumor ecosystem. How the interplay between tumor cells, EBV, and the microenvironment contributes to NPC progression and immune evasion remains unclear. Here we performed single-cell RNA sequencing on ~104,000 cells from 19 EBV + NPCs and 7 nonmalignant nasopharyngeal biopsies, simultaneously profiling the transcriptomes of malignant cells, EBV, stromal and immune cells. Overall, we identified global upregulation of interferon responses in the multicellular ecosystem of NPC. Notably, an epithelial-immune dual feature of malignant cells was discovered and associated with poor prognosis. Functional experiments revealed that tumor cells with this dual feature exhibited a higher capacity for tumorigenesis. Further characterization of the cellular components of the tumor microenvironment (TME) and their interactions with tumor cells revealed that the dual feature of tumor cells was positively correlated with the expression of co-inhibitory receptors on CD8 + tumor-infiltrating T cells. In addition, tumor cells with the dual feature were found to repress IFN-γ production by T cells, demonstrating their capacity for immune suppression. Our results provide new insights into the multicellular ecosystem of NPC and offer important clinical implications.
In this paper, the energy-time entangled photon-pairs at 1.5 μm are generated by the spontaneous four wave mixing (SFWM) in optical fibers under continuous wave (CW) pumping. The energy-time entanglement property is demonstrated experimentally through an experiment of Franson-type interference. Although the generation rates of the noise photons are one order of magnitude higher than that of the photon-pairs under CW pumping, the impact of noise photons can be highly suppressed in the measurement by a narrow time domain filter supported by superconducting nanowire single photon detectors with low timing jitters and time correlated single photon counting (TCSPC) module with high time resolution. The experiment results show that the SFWM in optical fibers under CW pumping provides a simple and practical way to generate energy-time entanglement at 1.5 μm, which has great potential for long-distance quantum information applications over optical fibers.
In this paper, hyper-entanglement on polarization and energy-time is generated based on a silicon micro-ring cavity. The silicon micro-ring cavity is placed in a fiber loop connected by a polarization beam splitter. Photon pairs are generated by the spontaneous four wave mixing (SFWM) in the cavity bi-directionally. The two photon states of photon pairs propagate along the two directions of the fiber loop and are superposed in the polarization beam splitter with orthogonal polarizations, leading to the polarization entanglement generation. On the other hand, the energy-time entanglement is an intrinsic property of photon pairs generated by the SFWM, which maintains in the process of the state superposition. The property of polarization entanglement is demonstrated by the two photon interferences under two non-orthogonal polarization bases. The property of energy-time entanglement is demonstrated by the Franson type interference under two non-orthogonal phase bases. The raw visibilities of all the measured interference fringes are higher than 1/2, the bench mark for violation of the Bell inequality. It indicates that silicon micro-ring cavity is a promising candidate to realize high performance hyper-entanglement generation.
In this Letter, telecom-band degenerate-frequency photon pairs are generated in a specific mode of a silicon microring cavity by the nondegenerate spontaneous four-wave mixing (SFWM) process, under two continuous-wave pumps at resonance wavelength of two different cavity modes. The ratio of coincidence to accidental coincidence is up to 100 under a time bin width of 5 ns, showing their characteristics of quantum correlation. Their quantum interference in balanced and unbalanced Mach-Zehnder interferometers is investigated theoretically and experimentally, and the results show potential in quantum metrology and quantum information.
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