Sustainably feeding a growing population is a grand challenge, and one that is particularly difficult in regions that are dominated by smallholder farming. Despite local successes, mobilizing vast smallholder communities with science- and evidence-based management practices to simultaneously address production and pollution problems has been infeasible. Here we report the outcome of concerted efforts in engaging millions of Chinese smallholder farmers to adopt enhanced management practices for greater yield and environmental performance. First, we conducted field trials across China's major agroecological zones to develop locally applicable recommendations using a comprehensive decision-support program. Engaging farmers to adopt those recommendations involved the collaboration of a core network of 1,152 researchers with numerous extension agents and agribusiness personnel. From 2005 to 2015, about 20.9 million farmers in 452 counties adopted enhanced management practices in fields with a total of 37.7 million cumulative hectares over the years. Average yields (maize, rice and wheat) increased by 10.8-11.5%, generating a net grain output of 33 million tonnes (Mt). At the same time, application of nitrogen decreased by 14.7-18.1%, saving 1.2 Mt of nitrogen fertilizers. The increased grain output and decreased nitrogen fertilizer use were equivalent to US$12.2 billion. Estimated reactive nitrogen losses averaged 4.5-4.7 kg nitrogen per Megagram (Mg) with the intervention compared to 6.0-6.4 kg nitrogen per Mg without. Greenhouse gas emissions were 328 kg, 812 kg and 434 kg CO equivalent per Mg of maize, rice and wheat produced, respectively, compared to 422 kg, 941 kg and 549 kg CO equivalent per Mg without the intervention. On the basis of a large-scale survey (8.6 million farmer participants) and scenario analyses, we further demonstrate the potential impacts of implementing the enhanced management practices on China's food security and sustainability outlook.

Bone marrow mesenchymal stem cells (MSCs) have improved cardiac performance when administered after acute myocardial infarction (MI) in both large-animal models and in patients.1,2 However, the results from randomized controlled clinical trials have been less impressive; the authors of one meta-analysis concluded that left ventricular ejection fractions (LVEFs) increased by just 2.92% in response to cell therapy.

Many hyperspectral vegetation indices (VIs) have been developed to estimate crop nitrogen (N) status at leaf and canopy levels. However, most of these indices have not been evaluated for estimating plant N concentration (PNC) of winter wheat (Triticum aestivum L.) at different growth stages using a common on-farm dataset. The objective of this study was to evaluate published VIs for estimating PNC of winter wheat in the North China Plain for different growth stages and years using data from both N experiments and farmers' fields, and to identify alternative promising hyperspectral VIs through a thorough evaluation of all possible two band combinations in the range of 350-1075 nm. Three field experiments involving different winter wheat cultivars and 4-6 N rates were conducted with cooperative farmers from 2005 to 2007 in Shandong Province, China. Data from 69 farmers' fields were also collected to evaluate further the published and newly identified hyperspectral VIs. The results indicated that best performing published and newly This is an ''enhanced'' version of the paper published as ''Comparing hyperspectral vegetation indices for estimating nitrogen concentration of winter wheat'' in E.J. van Henten, D. Goense and C. Lokhorst (Ed.) Precision Agriculture'09.identified VIs could explain 51% (R 700 /R 670 ) and 57% (R 418 /R 405 ), respectively, of the variation in PNC at later growth stages (Feekes 8-10), but only 22% (modified chlorophyll absorption ratio index, MCARI) and 43% (R 763 /R 761 ), respectively, at the early stages (Feekes 4-7). Red edge and near infrared (NIR) bands were more effective for PNC estimation at Feekes 4-7, but visible bands, especially ultraviolet, violet and blue bands, were more sensitive at Feekes 8-10. Across site-years, cultivars and growth stages, the combination of R 370 and R 400 as either simple ratio or a normalized difference index performed most consistently in both experimental (R 2 = 0.58) and farmers' fields (R 2 = 0.51). We conclude that growth stage has a significant influence on the performance of different vegetation indices and the selection of sensitive wavelengths for PNC estimation, and new approaches need to be developed for monitoring N status at early growth stages.

Increasing grain zinc (Zn) concentration of cereals for minimizing Zn malnutrition in two billion people represents an important global humanitarian challenge. Grain Zn in field-grown wheat at the global scale ranges from 20.4 to 30.5 mg kg−1, showing a solid gap to the biofortification target for human health (40 mg kg−1). Through a group of field experiments, we found that the low grain Zn was not closely linked to historical replacements of varieties during the Green Revolution, but greatly aggravated by phosphorus (P) overuse or insufficient nitrogen (N) application. We also conducted a total of 320-pair plots field experiments and found an average increase of 10.5 mg kg−1 by foliar Zn application. We conclude that an integrated strategy, including not only Zn-responsive genotypes, but of a similar importance, Zn application and field N and P management, are required to harvest more grain Zn and meanwhile ensure better yield in wheat-dominant areas.

The WRKY transcription factors (TFs) are one of the largest families of TFs in plants and play multiple roles in plant development and stress response. In the present study, GmWRKY16 encoding a WRKY transcription factor in soybean was functionally characterized in Arabidopsis. GmWRKY16 is a nuclear protein that contains a highly conserved WRKY domain and a C2H2 zinc-finger structure, and has the characteristics of transcriptional activation ability, presenting a constitutive expression pattern with relative expression levels of over fourfold in the old leaves, flowers, seeds and roots of soybean. The results of quantitative real time polymerase chain reaction (qRT-PCR) showed that GmWRKY16 could be induced by salt, alkali, ABA, drought and PEG-6000. As compared with the control, overexpression of GmWRKY16 in Arabidopsis increased the seed germination rate and root growth of seedlings in transgenic lines under higher concentrations of mannitol, NaCl and ABA. In the meantime, GmWRKY16 transgenic lines showed over 75% survival rate after rehydration and enhanced Arabidopsis tolerance to salt and drought with higher proline and lower MDA accumulation, less water loss of the detached leaves, and accumulated more endogenous ABA than the control under stress conditions. Further studies showed that AtWRKY8, KIN1, and RD29A were induced in GmWRKY16 transgenic plants under NaCl treatment. The expressions of the ABA biosynthesis gene (NCED3), signaling genes (ABI1, ABI2, ABI4, and ABI5), responsive genes (RD29A, COR15A, COR15B, and RD22) and stress-related marker genes (KIN1, LEA14, LEA76, and CER3) were regulated in transgenic lines under drought stress. In summary, these results suggest that GmWRKY16 as a WRKY TF may promote tolerance to drought and salt stresses through an ABA-mediated pathway.