The propanediol freezing method used in this study is capable of reproducibly preserving the developmental potential of human ovarian follicles. The developing follicle profile after cryopreservation cannot be accurately predicted from pre-freeze histology. Xenografting provides a powerful tool for assessing the potential of human cryopreserved ovarian tissue.

Follicles cryopreserved within human ovarian tissue using the PROH procedure, can develop to the antral stage and undergo periovulatory changes following xenografting and exposure to a luteinizing stimulus.

PurposeThere is a need for graduates who can quickly adjust and thrive within the current increasingly dynamic project-based workplaces. The purpose of this paper is to present the employers' perspectives on the work readiness of project management graduates entering the Australian construction industry.Design/methodology/approachTo gain the industry's perspective, qualitative in-depth interviews were conducted with key informants from 18 different construction companies that employ project management graduates.FindingsThematic analysis revealed that from the employers' perspective, work readiness constitutes (1) empathic communication, (2) passion and (3) technical construction knowledge. Graduates' areas of strength (e.g. application of technology) and weakness (e.g. responding to confrontational situations) were identified.Practical implicationsThe findings provide valuable insights into employers' perspectives of the work readiness of project management graduates which can serve as feedback to universities to assist in aligning their educational programmes with industry needs.Social implicationsWhile employers recognize that the responsibility for fostering work readiness should be shared between themselves and universities, this research highlights that currently adequate collaboration does not occur. This paper advocates for both universities and employers to be open to engaging in the conversation to enhance graduate work readiness.Originality/valueNo research to date has investigated the work readiness of project management graduates, nor whether their work readiness levels meet employers' requirements. This paper addresses this gap.

Undergraduate education is emerging as a direct pathway into the previously deemed ‘accidental profession’ of project management. We introduce the concept of graduate work readiness and explore whether undergraduate project management degrees are imparting industry-required work-readiness attributes. Documentary analysis of 12 Australian bachelor’s degree programs identified that many (but not all) of the work-ready attributes taught are consistent with those sought by industry. The results revealed that graduates’ work-readiness profiles vary according to university employer reputation. The findings suggest the need for a more industry-consultative approach to the way universities prepare their graduates for the project-based workplace.

BACKGROUND: The stage of folliculogenesis at which the human zona pellucida (ZP) is initiated and the cells responsible for the origin of the ZP continue to be controversial. This study characterizes the development of the ZP during human folliculogenesis using ovarian samples donated from patients requesting ovarian storage. METHODS: Follicles (from n 5 18 patients, 14-40 years old) within fresh tissue and following development in a xenograft system were stained, using immunohistochemical techniques, for the presence of the three human ZP proteins, ZP1, ZP2 and ZP3. Over 500 primordial follicles and >20 follicles at each developmental stage were examined. RESULTS: All three ZP proteins were detected within the oocyte of the primordial follicle. Presence of ZP1 and ZP3 was observed in the majority of primordial oocytes (93% and 95%, respectively), whereas ZP2 was detected in only 32% of these follicles. The three ZP proteins were detected in the cytoplasm of cuboidal granulosa cells and their distribution correlates with developmental stages throughout folliculogenesis. CONCLUSIONS: ZP proteins were detected in both the oocyte and the granulosa cells as early as the primordial follicle stage in the human. The detection of ZP proteins in the quiescent primordial follicle suggests that these proteins have been present since oogenesis.