This aim of this study was to distinguish grasslands from forests in southern Brazil by analyzing Poaceae pollen grains. Through light microscopy analysis, we measured the size of the pollen grain, pore, and annulus from 68 species of Rio Grande do Sul. Measurements were recorded of 10 forest species and 58 grassland species, representing all tribes of the Poaceae in Rio Grande do Sul. We measured the polar, equatorial, pore, and annulus diameter. Results of statistical tests showed that arboreous forest species have larger pollen grain sizes than grassland and herbaceous forest species, and in particular there are strongly significant differences between arboreous and grassland species. Discriminant analysis identified three distinct groups representing each vegetation type. Through the pollen measurements we established three pollen types: larger grains (>46 μm), from the Bambuseae pollen type, medium-sized grains (46–22 μm), from herbaceous pollen type, and small grains (<22 μm), from grassland pollen type. The results of our compiled Poaceae pollen dataset may be applied to the fossil pollen of Quaternary sediments.
Bee populations are declining globally due to different environmental stressors, such as pathogens, malnutrition, and agrochemicals. Brazil is the home of hundreds of stingless bee species, some of them now considered endangered, though very little is known about the impact of disease on native bees. In Southern Brazil the endangered stingless bee Melipona quadrifasciata is affected by an annual syndrome that causes sudden death of workers, eventually leading colonies to collapse.Although novel viruses were found in foragers from diseased colonies, none has been consistently implicated in the outbreak. Here we used transcriptomics in combination with an integrative longitudinal survey on managed colonies to identify predictors for preventing M. quadrifasciata colony failures. We found that key genes related to xenobiotic metabolization, nutrition and immune responses are downregulated in foragers from colonies that became diseased three months later. The period that preceded the outbreak was marked by pronounced forager weight loss as well as behavioural changes. Our findings support the proposition that worldwide bee mortality is influenced by a combination of diverse sublethal factors, and increase awareness of the long-term effects of genetic diversity erosion in stingless bee species, which enhances their vulnerability to environmental stressors.
Several South American species of Iridaceae, especially those of Tigridieae, produce floral oils as rewards to oil-bee pollinators. The present study aimed to contribute to a deeper understanding of the reproductive biology, pollination ecology and level of specialization of the interactions of species encompassed in Tigridieae. Data on breeding and pollination systems were acquired from six species native to Southern Brazil. The visitation frequency and pollen load of pollen- and oil-collecting bees were also investigated. The results strongly suggest that the studied species are distributed along a specialization–generalization continuum. Three oil-producing taxa, Cypella herbertii, Cypella pusilla and Cypella amplimaculata, were pollinated effectively by oil-bees, whereas in the other two studied species, Kelissa brasiliensis and Herbertia pulchella, the oil-bees appeared to function as oil thieves, owing to failure to contact the plant reproductive parts during oil-foraging behaviour. New insights into aspects of the specialization–generalization continuum of pollination systems, differences in pollinator behaviour during oil and pollen foraging, and reproductive outputs of the studied species are provided. Taken together, our results provide a significant contribution towards a better understanding of reproductive biology and plant–pollinator interactions between Iridaceae and oil-collecting bees.
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