Kuban state medical university, Krasnodar, russia В рамках экспериментального исследования оптимизирован протокол децеллюляризации пищевода на модели мелких лабораторных животных, изучена морфологическая структура полученного ацеллюлярного матрикса, проведена количественная оценка остатков ДНК, выполнена рецеллюляризация биологического каркаса мезенхимными мультипотентными стволовыми клетками для оценки цитотоксичности, сохранения клеточной жизнеспособности и метаболической активности, а также сделаны гетеротопические и ортотопические трансплантации децеллюляризированных и рецеллюляризированных матриксов крысам. Показано, что полученный децеллюляризированный матрикс пищевода сохраняет архитектонику нативной ткани при полном удалении клеточного материала, не обладает цитотоксическими свойствами, поддерживает адгезию и пролиферацию клеток и обладает проангиогенными свойствами, обусловленными, в том числе, сохранными компонентами внеклеточного матрикса, которые могут принимать активное участие в процессах неоангиогенеза и стимулировать микрососудистую пролиферацию эндотелиоцитов при гетеро-и ортотопической трансплантации. Это позволяет рассматривать разработанный и оптимизированный протокол децеллюляризации как перспективный способ получения биологического каркаса с проангиогенными свойствами для создания тканеинженерных конструкций и возможностью клинического применения в обозримом будущем. Ключевые слова: тканеинженерная конструкция, пищевод, децеллюляризированный матрикс, ангиогенез In the framework of the experimental study, the esophagus decellularization protocol was optimized in small laboratory animals, the morphological structure of the resulting acellular matrix was studied, the quantitative evaluation of residual DNa was made, the acellular scaffold was recellularized with mesenchymalmultipotent stem cells to assess cytotoxicity, maintain cell viability and metabolic activity, and heterotopic and orthotopic transplantations of decellularized and recellularized matrices in rats were performed. It is shown that the resulting decellularized esophagus matrix preserves the architectonics of native tissue with complete removal of cellular material, does not possess cytotoxic properties, supports adhesion and cell proliferation, and has proangiogenic properties, including preserved components of the extracellular matrix, which could take an active part in neoangiogenesisand stimulate microvascular proliferation of endotheliocytes in hetero-and orthotopic transplantation. all the facts allow us to consider the developed and modified protocol of esophageal decellularization to be a promising way to obtain a biological scaffold with pro-angiogenic properties for creating tissue engineered constructions and to give the possibility of clinical application in the foreseeable future.
Citation: Galenko-Yaroshevsky PA, Nefedov DA, Zelenskaya AV, Pavlyuchenko II, Chuyan EN, Ravaeva MY, Tkharkakhova NK (2018) Effects of Dimephosphone on skin survival in conditions of reduced blood circulation. Research Results in Pharmacology 4(4): 41-52. https://doi. AbstractIntroduction: The search for and creation of drugs with dermatoprotective and metabotropic activity is one of the priorities of modern diabetology. Synthetic organophosphorus compounds with no anticholinesterase activity, to which Dimephosphone belongs to, deserve great attention in this respect. Materials and Methods:Experiments included 355 white non-linear male mice (18-34 g) and 799 male rats (150-305 g). The dermatoprotective activity (DPA) of Dimephosphone regarding the survival of a skin graft was studied against the background of normoglycemia, as well as against the background of experimental diabetes complicated by hypercholesterolemia. The study of microhemodynamics in the skin was performed using laser Doppler flowmetry. The effects on metabolic processes and the antioxidant system were studied by determining the levels of glucose, urea, creatinine, total bilirubin, total cholesterol, triglycerides, total protein, albumin, globulin, catalase, malondialdehyde, superoxide dismutase, glutathione reductase, glutathione and glutathione peroxidase.Results: Dimephosphone has a pronounced DPA in conditions of reduced blood circulation against the background of normoglycemia and experimental (alloxan) diabetes complicated by exogenous hypercholesterolemia. By DPA, in most cases against the background of normoglycemia Dimephosphone exceeds Actovegine, is comparable to or inferior to Trental and Mexidol, and is more significant in terms of the therapeutic width than all the drugs taken for comparison.Discussion: According to the obtained data , DPA of Dimephosphone may be due to its ability to exhibit significant vasodilating, antihypoxic, antioxidant, antiaggregant, membrane-stabilizing, anti-acidotic, antimicrobial and other properties and also to exert a normalizing effect on carbohydrate, protein, lipid and energy metabolism Conclusion: Dimephosphone can be recommended for further preclinical and clinical studies in the form of various dosage forms, as well as in a combination therapy for metabolic disorders.
Medical news of north caucasus 2023. Vоl. 18. iss. 2
Introduction: The search for and development of new highly active medications and their combinations of the appropriate direction of action remains an urgent problem due to the complications of diabetes mellitus, especially burdened with atherosclerosis, including skin and vascular lesions. Materials and methods: The acute toxicity, histoprotective and dermatoprotective effects of mafusol, rexod, alprostadil and their combinations were studied in male rats with normoglycemia and alloxan diabetes complicated by exogenous hypercholesterolemia. Results: The combination of mafusol with rexod is less toxic than mafusol. In arteriovenous insufficiency of the tail, ischemia of the skin fold and skin flap, mafusol (6.25, 12.5 and 25.0 mg/kg in terms of fumarate), rexod (0.01 and 0.02 mg/kg) and especially their combination (6.25 and 0.01 mg/kg) have significant histoprotective, dermatoprotective, hypoglycemic and lipid-lowering effects, both in normoglycemia and alloxan diabetes complicated by exogenous hypercholesterolemia. Alprostadil (10 mg/kg) and especially its combination with mafusol (6.25 mg/kg) have a dermatoprotective effect. Discussion: Rexod reduces the acute toxicity of mafusol. The dermatoprotective effect of mafusol, rexod and, to a greater extent, their combination may be associated with increased microhemocirculation, antihypoxic properties and activation of energy processes in the skin, normalization of carbohydrate and lipid metabolism in alloxan diabetes, complicated by exogenous hypercholesterolemia, increased reserve capacity of the antioxidant system, and possibly with the ability of mafusol and rexod to reduce blood viscosity and improve rheological properties of the blood. The combination of mafusol with alprostadil increases the dermatoprotective activity of the latter. Conclusion: Combinations of mafusol with rexod and alprostadil can be recommended for clinical study as dermatoprotective agents for treating traumatic injuries and diabetes mellitus complicated by atherosclerosis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.