Gastrochilus matsuran (Makino) Schltr. (Orchidaceae) populations are declining quickly because of overexploitation, climatic changes, and deforestation; therefore, mass-production protocols are required for this orchid. Natural propagation of this species is often hampered by meager seed germination and slow growth. Thus, our aim was to establish an effective protocol for the in vitro propagation of G. matsuran and reduce the risk of its extinction. We investigated the impacts of culture media, coconut water (CW), and plant hormones (gibberellic acid (GA3), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA), and thidiazuron (TDZ)) on asymbiotic germination, multiplication and conversion of protocorms, and plantlet development. Maximal seed germination (93.3%) was achieved on ½ MS medium without vitamins plus 5% CW, 1 µM NAA, and 1.5 µM GA3. Secondary protocorm formation was best achieved on ½ MS medium without vitamins plus 2 µM TDZ. The conversion of protocorms into seedlings was maximized by supplementation with 2 µM IBA or 1 µM NAA. Acclimatized plantlets that exhibited exuberant growth on sphagnum moss were reintroduced to tree trunks in a natural habitat, with a 67% survival rate. This in vitro propagation procedure would be helpful for the mass production and conservation of this rare epiphytic orchid.
Electric utility companies (EUCs) play an intermediary role of retailers between wholesale market and end-users, maximizing their profits. Retail pricing can be well deployed with the support of EUCs to promote demand response (DR) programs for heating, ventilating, and air-conditioning (HVAC) systems in commercial buildings. This paper proposes a pricing strategy to help EUCs and building operators achieve an optimal DR of price-elastic HVAC systems, considering peak load reduction. The proposed strategy is implemented by adopting a bi-level decision model. The nonlinear thermal response of an experimental building room is modeled using piecewise linear equations, which helps convert the bi-level model to the single-level model. The pricing strategy is implemented considering a time-of-use (TOU) pricing scheme, leading to low price volatility. Case studies are conducted for two types of load curves and the results demonstrate that the proposed strategy helps EUC promote the price-based DR of the commercial buildings for conventional load curves. However, EUC cannot reduce the peak load on duck curve caused by the large introduction of photovoltaic generators, even with price-sensitive HVAC systems in commercial building. This will be addressed in future studies by inducing DR participation of HVAC systems in residential buildings.
A distribution system was designed and operated by considering unidirectional power flow from a utility source to end-use loads. The large penetrations of distributed generation (DG) into the existing distribution system causes a variety of technical problems, such as frequent tap changing problems of the on-load tap changer (OLTC) transformer, local voltage rise, protection coordination, exceeding short-circuit capacity, and harmonic distortion. In view of voltage regulation, the intermittent fluctuation of the DG output power results in frequent tap changing operations of the OLTC transformer. Thus, many utilities limit the penetration level of DG and are eager to find the reasonable penetration limits of DG in the distribution system. To overcome this technical problem, utilities have developed a new voltage regulation method in the distribution system with a large DG penetration level. In this paper, the impact of DG on the OLTC operations controlled by the line drop compensation (LDC) method is analyzed. In addition, a generalized determination methodology for the DG penetration limits in a distribution substation transformer is proposed. The proposed DG penetration limits could be adopted for a simplified interconnection process in DG interconnection guidelines.
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