No abstract

BackgroundMitochondrial dysfunction is associated with various aging diseases. The copy number of mtDNA in human cells may therefore be a potential biomarker for diagnostics of aging. Here we propose a new computational method for the accurate assessment of mtDNA copies from whole genome sequencing data.ResultsTwo families of the human whole genome sequencing datasets from the HapMap and the 1000 Genomes projects were used for the accurate counting of mitochondrial DNA copy numbers. The results revealed the parental mitochondrial DNA copy numbers are significantly lower than that of their children in these samples. There are 8%~21% more copies of mtDNA in samples from the children than from their parents. The experiment demonstrated the possible correlations between the quantity of mitochondrial DNA and aging-related diseases.ConclusionsSince the next-generation sequencing technology strives to deliver affordable and non-biased sequencing results, accurate assessment of mtDNA copy numbers can be achieved effectively from the output of whole genome sequencing. We implemented the method as a software package MitoCounter with the source code and user's guide available to the public at http://sourceforge.net/projects/mitocounter/.

Capacitive electrocardiogram (cECG) measurement is an attractive approach for long-term health monitoring. However, there is little literature available for the implementation of multiple-channel cECG system in standard limb leads. The circuit diagram for such a system is also rarely available in literature. This paper presents a multiple-channel limb-lead cECG system that utilized conductive fabrics as the capacitive sensors. The design criteria and the corresponding circuit diagram are described in detail. The proposed system also incorporates the capacitive driven-body (CDB) circuit to reduce the common-mode power-line interference (PLI). The presented system is verified to be stable by theoretic analysis and long-term experiments. The signals acquired by the presented system are competitive with those by commercially available electrocardiogram (ECG) machines. The feasible size and distance to the subject for the sensor made by conductive fabric have also been evaluated by a series of tests. From the test results, the sensor is suggested to be of greater than 60 cm2 in area and not more than 3 mm in distance for cECG measurement.