Results from the Collaborative Computational Project in Wave Structure Interaction (CCP-WSI) Blind Test Series 3 are presented. Participants, with numerical methods, ranging from low-fidelity linear models to high-fidelity Navier-Stokes (NS) solvers, simulate the interaction between focused waves and floating structures without prior access to the physical data. The waves are crest-focused NewWaves with various crest heights. Two structures are considered: a hemispherical-bottomed buoy and a truncated cylinder with a moon-pool; both are taut-moored with one linear spring mooring. To assess the predictive capability of each method, numerical results for heave, surge, pitch and mooring load are compared against corresponding physical data. In general, the NS solvers appear to predict the behaviour of the structures better than the linearised methods but there is considerable variation in the results (even between similar methods). Recommendations are made for future comparative studies and development of numerical modelling standards.

Osteosarcoma (OS) is an aggressive bone tumor, and proto-oncogene c-Fos is involved in this lethal disease. However, the role and molecular mechanism of c-Fos in the development and progression of OS remain enigmatic. As one of the Wnt family members, Wnt2 is closely associated with the development of several malignant tumors. In the present study, the expression of c-Fos, Wnt2, and its receptor Fzd9 in human OS tissues, MG63 OS cell line, and human osteoblast hFOB 1.19 cell line was detected by Western blot analysis, immunohistochemical staining, or reverse transcription-polymerase chain reaction. The role of c-Fos in the OS was clarified by treating MG63 cells with small interfering RNA to knockdown c-Fos. Then, cell migration and invasion were assayed by transwell assays and wound healing assay; cell proliferation was assayed by MTS method and 5-ethynyl-2'-deoxyuridine DNA proliferation in vitro detection; cell apoptosis was assayed by flow cytometric method. Co-immunoprecipitation kit was used to confirm the relationship between c-Fos and Wnt2/Fzd9. We found that the expression of c-Fos, Wnt2, and Fzd9 protein was distinctly higher in human OS tissues than that in the adjacent non-cancerous tissues, and their expression in the MG63 OS cell line was markedly increased compared with that in the human osteoblast hFOB 1.19 cell line. Knockdown of c-Fos inhibited the proliferation, migration, and invasion of MG63 cells, and promoted the apoptosis of MG63 cells. Moreover, knockdown of c-Fos inhibited the expression of Wnt2 and Fzd9 mRNA and protein. Our data enforced the evidence that knockdown of c-Fos inhibited cell proliferation, migration, and invasion, and promoted the apoptosis of OS cells accompanied by altered expression of Wnt2 and Fzd9. These findings offer new clues for OS development and progression, and c-Fos may be a potential therapeutic target for OS.

The paper presents results from the Collaborative Computational Project in Wave Structure Interaction (CCP-WSI) Blind Test Series 2. Without prior access to the physical data, participants, with numerical methods ranging from low-fidelity linear models to fully non-linear Navier–Stokes (NS) solvers, simulate the interaction between focused wave events and two separate, taut-moored, floating structures: a hemispherical-bottomed cylinder and a cylinder with a moonpool. The ‘blind’ numerical predictions for heave, surge, pitch and mooring load, are compared against physical measurements. Dynamic time warping is used to quantify the predictive capability of participating methods. In general, NS solvers and hybrid methods give more accurate predictions; however, heave amplitude is predicted reasonably well by all methods; and a WEC-Sim implementation, with CFD-informed viscous terms, demonstrates comparable predictive capability to even the stronger NS solvers. Large variations in the solutions are observed (even among similar methods), highlighting a need for standardisation in the numerical modelling of WSI problems.

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The resonant water motion inside a narrow gap between two identical fixed boxes that are in side-by-side configuration is investigated using a two-dimensional (2D) numerical wave tank based on OpenFOAM ® , an open source CFD package. Gap resonance is excited by regular waves with various wave heights, ranging from linear waves to strong nonlinear waves. This paper mainly focuses on the harmonic analyses of the free-surface elevation in the narrow gap and wave loads (including the horizontal wave forces, the vertical wave forces and the moments) on the bodies. It is found that the influences of the incident wave height on the higher-order harmonic components of different physical quantities are quite different. The effects of the incident wave height on the reflection, transmission and energy loss coefficients are also discussed. Finally, aiming at the quantitative estimation of the response time and the damping time of gap resonance, two different methods are proposed and verified for the first time on gap resonance.