Highlights d Rapid establishment of glioblastoma organoids (GBOs) in a defined medium with biobank d GBOs maintain parental tumor cellular heterogeneity, gene expression, and mutations d GBO transplantation exhibits efficient engraftment and aggressive infiltration d Tumor-specific treatment responses in GBOs to drugs and CART cells

Highlights d SNOs maintain growth and laminar expansion over long-term culture d SNOs exhibit separated upper and deep cortical layers d Layer-specific WNT/b-catenin signaling regulates neuronal fate specification d DISC1 mutation causes deficits in cortical neuron fate specification

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