Autophagy is a homeostatic process with multiple functions in mammalian
cells. Here we show that mammalian Atg8 proteins (mAtg8s) and the autophagy
regulator IRGM control TFEB, a transcriptional activator of the lysosomal
system. IRGM directly interacted with TFEB and promoted TFEB’s nuclear
translocation. An mAtg8 partner of IRGM, GABARAP, interacted with TFEB. Deletion
of all mAtg8s or GABARAPs affected global transcriptional response to starvation
and down-regulated subsets of TFEB targets. IRGM and GABARAPs countered
mTOR’s action as a negative regulator of TFEB. This was suppressed by
constitutively active RagB, an activator of mTOR. Infection of macrophages with
membrane-permeabilizing microbe
Mycobacterium tuberculosis
or
infection of target cells by HIV elicited TFEB activation in an IRGM-dependent
manner. Thus, IRGM and its interactors mAtg8s close a loop between the
autophagosomal pathway and the control of lysosomal biogenesis by TFEB ensuring
coordinated activation of the two systems that eventually merge during
autophagy.
The integral membrane protein ATG9A plays a key role in autophagy. It displays a broad intracellular distribution and is present in numerous compartments, including the plasma membrane (PM). The reasons for the distribution of ATG9A to the PM and its role at the PM are not understood. Here, we show that ATG9A organizes, in concert with IQGAP1, components of the ESCRT system and uncover cooperation between ATG9A, IQGAP1 and ESCRTs in protection from PM damage. ESCRTs and ATG9A phenocopied each other in protection against PM injury. ATG9A knockouts sensitized the PM to permeabilization by a broad spectrum of microbial and endogenous agents, including gasdermin, MLKL and the MLKL-like action of coronavirus ORF3a. Thus, ATG9A engages IQGAP1 and the ESCRT system to maintain PM integrity.
ACE2 and TMPRSS2 are key players on SARS-CoV-2 entry into host cells. However, it is still unclear whether expression levels of these factors could reflect disease severity. Here, a case–control study was conducted with 213 SARS-CoV-2 positive individuals where cases were defined as COVID-19 patients with respiratory distress requiring oxygen support (N = 38) and controls were those with mild to moderate symptoms of the disease who did not need oxygen therapy along the entire clinical course (N = 175). ACE2 and TMPRSS2 mRNA levels were evaluated in nasopharyngeal swab samples by RT-qPCR and logistic regression analyzes were applied to estimate associations with respiratory outcomes. ACE2 and TMPRSS2 levels positively correlated with age, which was also strongly associated with respiratory distress. Increased nasopharyngeal ACE2 levels showed a protective effect against this outcome (adjOR = 0.30; 95% CI 0.09–0.91), while TMPRSS2/ACE2 ratio was associated with risk (adjOR = 4.28; 95% CI 1.36–13.48). On stepwise regression, TMPRSS2/ACE2 ratio outperformed ACE2 to model COVID-19 severity. When nasopharyngeal swabs were compared to bronchoalveolar lavages in an independent cohort of COVID-19 patients under mechanical ventilation, similar expression levels of these genes were observed. These data suggest nasopharyngeal TMPRSS2/ACE2 as a promising candidate for further prediction models on COVID-19.
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