We propose an Adaptive Optics Light-Sheet Fluorescence Microscope (AO-LSFM) for closed-loop aberrations correction at the emission path, providing intrinsic instrumental simplicity and high accuracy when compared to previously reported schemes. The approach is based on direct wavefront sensing i.e. not on time-consuming iterative algorithms, and does not require the use of any guide star thus reducing instrumental complexity and/or sample preparation constraints. The design is based on a modified Shack-Hartmann wavefront sensor providing compatibility with extended sources such as images from optical sectioning microscopes. We report an AO-LSFM setup based on such sensor including characterization of the sensor performance, and demonstrate for the first time significant contrast improvement on neuronal structures of the ex-vivo adult drosophila brain in depth.
Adaptive optics (AO) has been implemented on several microscopy setups and has proven its ability to increase both signal and resolution. However, reported configurations are not suited for fast imaging of live samples or are based on an invasive or complex implementation method.Aim: Provide a fast aberration correction method with an easy to implement AO module compatible with light-sheet fluorescence microscopy (LSFM) for enhanced imaging of live samples.Approach: Development of an AO add-on module for LSFM based on direct wavefront sensing without requiring a guide star using an extended-scene Shack-Hartmann wavefront sensor. The enhanced setup uses a two-color sample labeling strategy to optimize the photon budget.Results: Fast AO correction of in-depth aberrations in an ex-vivo adult Drosophila brain enables doubling the contrast when imaging with either cell reporters or calcium sensors for functional imaging. We quantify the gain in terms of image quality on different functional domains of sleep neurons in the Drosophila brain at various depths and discuss the optimization of key parameters driving AO.
Conclusion:We developed a compact AO module that can be integrated into most of the reported light-sheet microscopy setups, provides significant improvement of image quality and is compatible with fast imaging requirements such as calcium imaging.
We report on an Adaptive Optics (AO) Light-Sheet Fluorescence Microscope compatible with neuroimaging, based on direct wavefront sensing without the requirement of a guide star. We demonstrate fast AO correction, typically within 500ms, of in-depth aberrations of the live adult Drosophila Melanogaster brain, enabling to double the contrast when imaging with structural or calcium sensors. We quantify the gain in terms of image quality on multiply neuronal structures part of the sleep network in the Drosophila brain, at various depths, and discuss the optimization of key parameters driving AO such as the number of corrected modes and the photon budget. We present a first design of a compact AO add-on that is compatible with integration into most of reported Light-Sheet setups and neuroimaging.
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