BackgroundThe production and employment of cellulases still represents an economic bottleneck in the conversion of lignocellulosic biomass to biofuels and other biocommodities. This process could be simplified by displaying the necessary enzymes on a microbial cell surface. Such an approach, however, requires an appropriate host organism which on the one hand can withstand the rough environment coming along with lignocellulose hydrolysis, and on the other hand does not consume the generated glucose so that it remains available for subsequent fermentation steps.ResultsThe robust soil bacterium Pseudomonas putida showed a strongly reduced uptake of glucose above a temperature of 50 °C, while remaining structurally intact hence recyclable, which makes it suitable for cellulose hydrolysis at elevated temperatures. Consequently, three complementary, thermophilic cellulases from Ruminiclostridium thermocellum were displayed on the surface of the bacterium. All three enzymes retained their activity on the cell surface. A mixture of three strains displaying each one of these enzymes was able to synergistically hydrolyze filter paper at 55 °C, producing 20 μg glucose per mL cell suspension in 24 h.ConclusionWe could establish Pseudomonas putida as host for the surface display of cellulases, and provided proof-of-concept for a fast and simple cellulose breakdown process at elevated temperatures. This study opens up new perspectives for the application of P. putida in the production of biofuels and other biotechnological products.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-016-0505-8) contains supplementary material, which is available to authorized users.
We evolved the thermophilic β-glucosidase CsBglA to 150% improvement of kcat/KM at 55 °C, exerting a specific activity of 504 U mg−1 and a KM-value of 37.1 mM, making it the bacterial β-glucosidase with highest activity known so far.
Summary
The application of enzymes as biocatalysts in industrial processes has great potential due to their outstanding stereo‐, regio‐ and chemoselectivity. Using autodisplay, enzymes can be immobilized on the cell surface of Gram‐negative bacteria such as Escherichia coli. In the present study, the surface display of an alcohol dehydrogenase (ADH) and a cyclohexanone monooxygenase (CHMO) on E. coli was investigated. Displaying these enzymes on the surface of E. coli resulted in whole‐cell biocatalysts accessible for substrates without further purification. An apparent maximal reaction velocity VMAX(app) for the oxidation of cyclohexanol with the ADH whole‐cell biocatalysts was determined as 59.9 mU ml−1. For the oxidation of cyclohexanone with the CHMO whole‐cell biocatalysts a VMAX(app) of 491 mU ml−1 was obtained. A direct conversion of cyclohexanol to ε‐caprolactone, which is a known building block for the valuable biodegradable polymer polycaprolactone, was possible by combining the two whole‐cell biocatalysts. Gas chromatography was applied to quantify the yield of ε‐caprolactone. 1.12 mM ε‐caprolactone was produced using ADH and CHMO displaying whole‐cell biocatalysts in a ratio of 1:5 after 4 h in a cell suspension of OD578nm 10. Furthermore, the reaction cascade as applied provided a self‐sufficient regeneration of NADPH for CHMO by the ADH whole‐cell biocatalyst.
CK2β is the non‐catalytic modulating part of the S/T‐protein kinase CK2. However, the overall function of CK2β is poorly understood. Here, we report on the identification of 38 new interaction partners of the human CK2β from lysates of DU145 prostate cancer cells using photo‐crosslinking and mass spectrometry, whereby HSP70‐1 was identified with high abundance. The KD value of its interaction with CK2β was determined as 0.57 μM by microscale thermophoresis, this being the first time, to our knowledge, that a KD value of CK2β with another protein than CK2α or CK2α′ was quantified. Phosphorylation studies excluded HSP70‐1 as a substrate or activity modulator of CK2, suggesting a CK2 activity independent interaction of HSP70‐1 with CK2β. Co‐immunoprecipitation experiments in three different cancer cell lines confirmed the interaction of HSP70‐1 with CK2β in vivo. A second identified CK2β interaction partner was Rho guanin nucleotide exchange factor 12, indicating an involvement of CK2β in the Rho‐GTPase signal pathway, described here for the first time to our knowledge. This points to a role of CK2β in the interaction network affecting the organization of the cytoskeleton.
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