The purpose of the current study was to evaluate the effects of inoculants on chemical composition, dry matter (DM) and neutral detergent fiber (aNDF) in vitro degradation, fermentative and effluent losses, microbiology, fermentative profile, and aerobic stability of sugarcane mini-silos. Treatments were randomly distributed to the mini-silos, in which: (1) Control (CON); (2) Lactobacillus buchneri (Lb), addition of Lb at 2.6 × 10 10 cfu/g; (3) Lactobacillus buchneri and Bacillus subtilis (Lb + Bs), addition of Lb at 2.6 × 10 10 cfu/g and Bs at 1 × 10 9 cfu/g; and (4) Chitosan (CHI), addition of 1% of CHI on wet basis of sugarcane ensiled. Treatments 2 and 3 were incorporated to the silage at 2 g/t of natural matter ensiled. Lb and Lb + Bs did not alter the in vitro degradation of DM and NDF. Chitosan incorporation increased the DM content (P = 0.013, 18.7 g/kg DM) and improved (P = 0.029, 45.6 g/kg DM) the NDF in vitro degradation of sugarcane silage. In addition, CHI incorporation showed higher (P = 0.002) DM content in silage than Lb and Lb + Bs. Microbial inoculants (Lb and Lb + Bs) reduced the total losses (P = 0.009) of sugarcane silage. Moreover, CHI incorporation showed lower (P = 0.001, 84.9 g/kg DM) total losses and higher (P = 0.031, 84.8 g/kg DM) dry matter recovery than Lb and Lb + Bs. Lactic acid bacteria concentration was increased (P = 0.001) with additives, and CHI incorporation showed higher (P = 0.001) lactic acid bacteria concentration than silages treated Lb and Lb + Bs. All additives decreased the ethanol concentration in sugarcane silage, but CHI showed lower (P = 0.002) ethanol concentration compared to Lb and Lb + Bs. Inoculants improved the aerobic stability of sugarcane silage. In general, the incorporation of CHI to sugarcane silage showed better results of NDF in vitro degradation and gas and effluent losses than Lb and Lb + Bs. Moreover,

-This study was undertaken to determine the effects of chitosan and whole raw soybean on nutrient intake and total tract digestion, nitrogen utilization, microbial protein synthesis, blood metabolites, and energy balance of dairy heifers. Twelve Jersey heifers (6±0.5 months of age and 139.50±25.56 kg of live weight; mean ± standard deviation) were randomly assigned to a replicated Latin square design with a 2 × 2 factorial arrangement. The experimental period consisted of 14 days of adaptation to diets, six days of sampling, and five days of washout. The experimental diets were: control (CO); chitosan (CHI, inclusion of 2.0 g kg −1 DM of chitosan); whole raw soybean (WS, 163.0 g kg −1 of WS on diet DM basis); and chitosan + whole raw soybean (CHI+WS). Chitosan decreased dry matter and neutral detergent fiber intakes; however, CHI increased DM total tract digestion. An interaction effect was observed on retained nitrogen, which increased when animals were fed CHI+WS compared with CO or CHI, but did not differ from that of animals fed WS. Chitosan decreased microbial nitrogen and crude protein flow of heifers. Energy balance was improved when heifers received diets containing WS. Efficiency of energy utilization was not affected by experimental diets. An interaction effect was observed for blood high-density lipoprotein (HDL) concentration, which increased with both dietary inclusion of CHI and WS compared with the other diets, and CHI provided the lowest value of HDL cholesterol. Chitosan and whole raw soybean do not alter nutrient intake and total tract digestion; however, they decrease nitrogen urinary excretion and increase blood HDL cholesterol of heifers.

RESUMO. Avaliou-se a degradabilidade ruminal in situ, em ovinos dos grãos e coprodutos de girassol, soja e crambe, em três ovinos da raça Santa Inês com peso de 40 kg, fistulados e providos de cânulas ruminais, mantidos em baias individuais. Os alimentos foram incubados em ordem decrescente de 72, 48, 24, 18, 6, 3 e 0h. A fração potencialmente degradável da MS para a torta de girassol foi 73,7% e da PB 34,6%, o que proporcionou média degradação ruminal para PB. O grão de girassol apresentou baixa degradabilidade ruminal para a MS e PB (33,55 e 25,98%). A torta de soja apresentou fração solúvel de 23,15 e 7,79%, para a MS e PB, com uma degradabilidade efetiva de 75,6 e 70,89%, enquanto que o grão de soja apresentou baixa fração solúvel para MS e PB (4,79 e 8,73%), acarretando baixa degradabilidade ruminal. O grão de crambe e a torta de crambe apresentaram os maiores teores de FDA, e baixa degradabilidade ruminal, tanto para a MS como para a PB. Os valores para a fração potencialmente degradável e fração solúvel foram semelhantes. Os alimentos avaliados apresentaram média degradabilidade ruminal, exceto a torta de soja que apresentou maior valor de degradabilidade ruminal.Palavras-chave: composição química, matéria seca, proteína, sacos de náilon, tempo de colonização.ABSTRACT. In situ ruminal degradability of crambe, sunflower and soybean seeds and their by-products in sheep feeding. Ruminal degradability of crambe, sunflower and soybean grains and their by-products in sheep was evaluated by an "in situ" technique. Three 40 kg-Santa Ines fistulated sheep provided with ruminal cannula and kept in individual pens were analyzed. Feeds were incubated in the rumen in the following decreasing order 72, 48, 24, 18, 6, 3 and 0h. The potentially degradable fraction for DM in sunflower meal amounted to 73.7%, whereas it reached 34.6% in the case of CP, with medium ruminal degradation. Sunflower grain had low ruminal degradability for DM and CP (33.55 and 25.98%). Crushed soybean grain had soluble fraction of 23.15 and 7.79% respectively for DM and CP, with an effective degradability of 75.6 and 70.89%. Further, soybean whole grain presented low soluble fraction for DM and CP, respectively 4.79 and 8.73%, with low ruminal degradability. Crambe whole grain and crushed meal had the highest ADF rates, with low ruminal degradability for DM and CP. Rates for potentially degradable and soluble fraction were similar. Evaluated feeds had medium ruminal degradability, except for soybean crushed meal with the highest rate in ruminal degradability.

Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects. Chitosan has been evaluated as rumen modulator and silage additive due to its antimicrobial properties. The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage. Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO. Microbial inoculant was composed of Lactobacillus plantarum (4.0 × 10 cfu/g) and Propionibacterium acidipropionici (2.6 × 10 cfu/g). The CHI and INO alone increased counts of lactic bacteria and anaerobic bacteria and decreased counts of mold and yeast in SS. The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS. Chitosan increased NH-N and lactate concentrations and decreased ethanol concentration in SS. The CHI increased dry matter recovery from SS; INO increased silage aerobic stability. The combination of CHI+INO showed the lowest value of gas losses. In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS. Chitosan or INO utilization improves SS quality.

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RESUMO. Avaliou-se a degradabilidade in situ, dos grãos de linhaça, canola, colza e milho, caroço de algodão, e dos concentrados casca e farelo de soja, em ovinos. Foram utilizados três animais da raça Santa Inês, fistulados e providos de cânulas ruminais, mantidos em baias individuais, recebendo diariamente capim picado e ração concentrada. Os alimentos foram incubados em ordem decrescente de 72, 48, 24, 18, 6, 3 e 0h. O teor de proteína dos alimentos avaliados foram 34,77; 30,07; 23,70; 10,64; 26,12; 14,65 e 56,90% para canola, colza, linhaça, milho, caroço de algodão, casca de soja e farelo de soja, respectivamente. A canola e a colza apresentaram baixa degradabilidade efetiva para a MS, com valor médio de 33,68%. O grão de linhaça apresentou degradabilidade efetiva para a MS de 64,24%, com fração potencialmente degradável de 87,89%. O milho apresentou fração solúvel de 12,33% e uma degradação de 39,67% para a MS. O farelo de soja apresentou-se dentro dos parâmetros normais de degradação com 52,61% para a MS e 52,83% para PB. Para a linhaça, canola e colza a degradabilidade da proteína apresentou valor médio de 18,34%. Os grãos de avaliados apresentaram baixa degradabilidade efetiva para a matéria seca e proteína bruta.Palavras-chave: composição centesimal, colza, linhaça, in situ.ABSTRACT. Ruminal degradability of dry matter and crude protein, and microbial colonization time of oil grains in sheep feeding. This study evaluated in situ ruminal degradability of grains of linseed, canola, rapeseed and corn, whole cottonseed, as well as soybean hulls and soybean meal, in sheep. Three Santa Inês sheep were fistulated and fitted with rumen cannulas. The animals were housed in individual stalls, receiving chopped grass and concentrated feed daily. Feeds were incubated in descending order of 72, 48, 24, 18, 6, 3 and 0h. Protein content was 34.77% for canola, 30.07% for rapeseed, 23.70% for linseed, 10.64% for corn, 26.12% for cottonseed, 14.65% for soybean hulls, and 56.90% for soybean meal. Canola and rapeseed showed low effective degradability of DM, with mean value of 33.68%. Linseed grain had DM degradability of 64.24%, with 87.89% potential degradable fraction. Corn grain showed soluble fraction of 12.33% and 39.67% degradation for DM. Soybean meal was within normal parameters of degradation, with 52.61% for DM and 52.83% for CP. For linseed, canola and rapeseed, average protein degradability value was 18.34%. The evaluated grains showed low effective degradability for dry matter and crude protein.

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