SUMMARY We discovered a class of naturally occurring human proteins with unusually high net positive charge that can potently deliver proteins in functional form into mammalian cells both in vitro, and also in murine retina, pancreas, and white adipose tissues in vivo. These findings represent new, diverse macromolecule delivery agents for in vivo applications, and also raise the possibility that some of these human proteins may penetrate cells as part of their native biological functions.

Measuring the abundances of carbon and oxygen in exoplanet atmospheres is considered a crucial avenue for unlocking the formation and evolution of exoplanetary systems1,2. Access to the chemical inventory of an exoplanet requires high-precision observations, often inferred from individual molecular detections with low-resolution space-based3–5 and high-resolution ground-based6–8 facilities. Here we report the medium-resolution (R ≈ 600) transmission spectrum of an exoplanet atmosphere between 3 and 5 μm covering several absorption features for the Saturn-mass exoplanet WASP-39b (ref. 9), obtained with the Near Infrared Spectrograph (NIRSpec) G395H grating of JWST. Our observations achieve 1.46 times photon precision, providing an average transit depth uncertainty of 221 ppm per spectroscopic bin, and present minimal impacts from systematic effects. We detect significant absorption from CO2 (28.5σ) and H2O (21.5σ), and identify SO2 as the source of absorption at 4.1 μm (4.8σ). Best-fit atmospheric models range between 3 and 10 times solar metallicity, with sub-solar to solar C/O ratios. These results, including the detection of SO2, underscore the importance of characterizing the chemistry in exoplanet atmospheres and showcase NIRSpec G395H as an excellent mode for time-series observations over this critical wavelength range10.

Bean et al.

Transmission spectroscopy 1,2,3 of exoplanets has revealed signatures of water vapor, aerosols, and alkali metals in a few dozen exoplanet atmospheres 4,5 . However, these previous inferences with the Hubble and Spitzer Space Telescopes were hindered by the observations' relatively narrow wavelength range and spectral resolving power, which precluded the unambiguous identification of other chemical species -in particular the primary carbon-bearing molecules 6,7 . Here we report a broad-wavelength 0.5-5.5 µm atmospheric transmission spectrum of WASP39 b 8 , a 1200 K, roughly Saturn-mass, Jupiter-radius exoplanet, measured with JWST NIRSpec's PRISM mode 9 as part of the JWST Transiting Exoplanet Community Early Release Science Team program 10,11,12 . We robustly detect multiple chemical species at high significance, including Na (19σ), H 2 O (33σ), CO 2 (28σ), and CO (7σ). The non-detection of CH 4 , combined with a strong CO 2 feature, favours atmospheric models with a super-solar atmospheric metallicity. An unanticipated absorption feature at 4µm is best explained by SO 2 (2.7σ), which could be a tracer of atmospheric photochemistry. These observations demonstrate JWST's sensitivity to a rich diversity of exoplanet compositions and chemical processes.We observed one transit of WASP-39b on 10 July 2022 with JWST's Near InfraRed Spectrograph (NIRSpec) 9,13 , using the PRISM mode, as part of the JWST Transiting Exoplanet Community Early Release Science Program (ERS Program 1366) (PIs: N. Batalha, J. Bean, K. Stevenson) 10,11 . These observations cover the 0.5-5.5µm wavelength range at a native resolving power of R = λ/∆λ ∼ 20-300. WASP-39b was selected for this JWST ERS Program due to previous space-and ground-based observations revealing strong alkali metal absorption and multiple prominent H 2 O bands 4,6,14,15,16 , suggesting strong signal-to-noise could be obtained with JWST. However, the limited wavelength range of existing transmission spectra (0.3-1.65µm, combined with two wide photometric Spitzer channels at 3.6 and 4.5µm) left several important questions unresolved. Previous estimates of WASP-39b's atmospheric metallicity-a measure of the relative abundance of all gases heavier than hydrogen or helium-vary by four orders of magnitude 6,16,17,18,19,20 . Accurate determinations of metallicity can elucidate formation pathways and provide greater insight into the planet's history 21 . The JWST NIRSpec PRISM observations we present here offer a more detailed view into WASP-39b's atmospheric composition than has previously been possible (see ref. 21 for an initial infrared analysis of this data).We obtained time-series spectroscopy over 8.23 hours centered around the transit event to extract the wavelength-dependent absorption by the planet's atmosphere-i.e., the transmission spectrum, which probes the planet's day-night terminator region near millibar pressures. We used NIRSpec PRISM in Bright Object Time Series (BOTS) mode. WASP-39 is a bright, nearby, relatively inactive 23 G7 type star with an effective tempe...

The Saturn-mass exoplanet WASP-39b has been the subject of extensive efforts to determine its atmospheric properties using transmission spectroscopy1–4. However, these efforts have been hampered by modelling degeneracies between composition and cloud properties that are caused by limited data quality5–9. Here we present the transmission spectrum of WASP-39b obtained using the Single-Object Slitless Spectroscopy (SOSS) mode of the Near Infrared Imager and Slitless Spectrograph (NIRISS) instrument on the JWST. This spectrum spans 0.6–2.8 μm in wavelength and shows several water-absorption bands, the potassium resonance doublet and signatures of clouds. The precision and broad wavelength coverage of NIRISS/SOSS allows us to break model degeneracies between cloud properties and the atmospheric composition of WASP-39b, favouring a heavy-element enhancement (‘metallicity’) of about 10–30 times the solar value, a sub-solar carbon-to-oxygen (C/O) ratio and a solar-to-super-solar potassium-to-oxygen (K/O) ratio. The observations are also best explained by wavelength-dependent, non-grey clouds with inhomogeneous coverageof the planet’s terminator.

While Spitzer Infrared Array Camera (IRAC) systematics are generally well understood, each data set can provide its own challenges that continue to teach us about the underlying functional form of these systematics. Multiple groups have analyzed the phase curves of WASP-43b with varying detrending techniques, each obtaining different results. In this work, we take another look at WASP-43b while exploring the degenerate relation between Bilinearly Interpolated Subpixel Sensitivity (BLISS) mapping, point response function (PRF)–FWHM detrending, and phase curve parameters. We find that there is a strong correlation between the detrending parameters in the two models, and best-fit phase curve amplitudes vary strongly when the data are temporally binned. To remove this degeneracy, we present a new Gaussian centroided intrapixel sensitivity map (hereafter fixed sensitivity map), generated using 3,712,830 exposures spanning 5 yr, for a variety of aperture sizes at 4.5 μm. We find evidence for time variability in the sensitivity at 3.6 μm and do not generate a visit-independent map for this channel. With the fixed 4.5 μm intrapixel sensitivity map, the best fits for WASP-43b no longer vary strongly with bin size and PRF–FWHM detrending is no longer required to remove correlated noise. For data sets that do not fall completely within the sweet spot, temporal binning should not be used in the analysis of Spitzer phase curves. We confirm nightside emission for WASP-43b with a disk-integrated nightside temperature of 806 ± 48 K at 4.5 μm. The 4.5 μm maps are available at github.com/kevin218/POET.

The large radii of many hot Jupiters can only be matched by models that have hot interior adiabats, and recent theoretical work has shown that the interior evolution of hot Jupiters has a significant impact on their atmospheric structure. Due to its inflated radius, low gravity, and ultrahot equilibrium temperature, WASP-76b is an ideal case study for the impact of internal evolution on observable properties. Hot interiors should most strongly affect the nonirradiated side of the planet, and thus full phase-curve observations are critical to ascertain the effect of the interior on the atmospheres of hot Jupiters. In this work, we present the first Spitzer phase-curve observations of WASP-76b. We find that WASP-76b has an ultrahot dayside and relatively cold nightside with brightness temperatures of 2471 ± 27 K/1518 ± 61 K at 3.6 μm and 2699 ± 32 K/1259 ± 44 K at 4.5 μm, respectively. These results provide evidence for a dayside thermal inversion. Both channels exhibit small phase offsets of 0.68 ± 0.°48 at 3.6 μm and 0.67 ± 0.°2 at 4.5 μm. We compare our observations to a suite of general circulation models (GCMs) that consider two endmembers of interior temperature along with a broad range of frictional drag strengths. Strong frictional drag is necessary to match the small phase offsets and cold nightside temperatures observed. From our suite of cloud-free GCMs, we find that only cases with a cold interior can reproduce the cold nightsides and large phase-curve amplitude at 4.5 μm, hinting that the hot interior adiabat of WASP-76b does not significantly impact its atmospheric dynamics or that clouds blanket its nightside.

Measuring the metallicity and carbon-to-oxygen (C/O) ratio in exoplanet atmospheres is a fundamental step towards constraining the dominant chemical processes at work and, if in equilibrium, revealing planet formation histories. Transmission spectroscopy (for example, refs. 1,2) provides the necessary means by constraining the abundances of oxygen- and carbon-bearing species; however, this requires broad wavelength coverage, moderate spectral resolution and high precision, which, together, are not achievable with previous observatories. Now that JWST has commenced science operations, we are able to observe exoplanets at previously uncharted wavelengths and spectral resolutions. Here we report time-series observations of the transiting exoplanet WASP-39b using JWST’s Near InfraRed Camera (NIRCam). The long-wavelength spectroscopic and short-wavelength photometric light curves span 2.0–4.0 micrometres, exhibit minimal systematics and reveal well defined molecular absorption features in the planet’s spectrum. Specifically, we detect gaseous water in the atmosphere and place an upper limit on the abundance of methane. The otherwise prominent carbon dioxide feature at 2.8 micrometres is largely masked by water. The best-fit chemical equilibrium models favour an atmospheric metallicity of 1–100-times solar (that is, an enrichment of elements heavier than helium relative to the Sun) and a substellar C/O ratio. The inferred high metallicity and low C/O ratio may indicate significant accretion of solid materials during planet formation (for example, refs. 3,4,) or disequilibrium processes in the upper atmosphere (for example, refs. 5,6).