Using benzylidene imidazolone core, we created ap anel of color-shifted fluorogenic ligandsf or FAST protein withoutc ompromise to the binding efficiency and the utility for live-cellp rotein labeling. Thiss tudy highlights the potential of benzylidene imidazolones derivatives for rapid expansion of ap allet of live-cellf luorogenic labelingt ools.

(2-Aminobenzylidene)-imidazolones were used as substrates for [1,5]-hydride shift triggered cyclization under promotion by TiCl4 at room temperature.

An efficient and high-yielding strategy to prepare “unsymmetrical” 4-aryl-isoxazol-3,5-dicarboxylic acid derivatives from nitroacetic esters and aromatic aldehydes has been developed. The strategy is based on the isolation and usage of the previously missed intermediate of the Dornow reaction5-hydroxy-6-oxo-4-aryl-6H-1,2-oxazine-3-carboxylates. In addition, the mechanism of the Dornow reaction was partially revised.