Using benzylidene imidazolone core, we created ap anel of color-shifted fluorogenic ligandsf or FAST protein withoutc ompromise to the binding efficiency and the utility for live-cellp rotein labeling. Thiss tudy highlights the potential of benzylidene imidazolones derivatives for rapid expansion of ap allet of live-cellf luorogenic labelingt ools.
An efficient and high-yielding strategy to prepare “unsymmetrical”
4-aryl-isoxazol-3,5-dicarboxylic acid derivatives from nitroacetic
esters and aromatic aldehydes has been developed. The strategy is
based on the isolation and usage of the previously missed intermediate
of the Dornow reaction5-hydroxy-6-oxo-4-aryl-6H-1,2-oxazine-3-carboxylates. In addition, the mechanism of the Dornow
reaction was partially revised.
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