SUMMARYThe mechanism underlying the decrease in aerobic scope in fish at warm temperatures is not fully understood and is the focus of this research. Our study examined oxygen uptake and delivery in resting, swimming and recovering sockeye salmon while water temperature was acutely increased from 15°C to 24°C in 2°C h -1 increments. Fish swam at a constant speed during the temperature change. By simultaneously measuring oxygen consumption (M O2 ), cardiac output (Q) and the blood oxygen status of arterial and venous blood, we were able to determine where in the oxygen cascade a limitation appeared when fish stopped sustained swimming as temperature increased. High temperature fatigue of swimming sockeye salmon was not a result of a failure of either oxygen delivery to the gills or oxygen diffusion at the gills because oxygen partial pressure (P O2 ) and oxygen content (C O2 ) in arterial blood did not decrease with increasing temperature, as would be predicted for such limitations. Instead, arterial oxygen delivery (Ta O2 ) was initially hampered due to a failure to adequately increase Q with increasing temperature. Subsequently, lactate appeared in the blood and venous P O2 remained constant.
Over the past several decades, a substantial amount of research has examined how cardiorespiratory physiology supports the diverse activities performed throughout the life cycle of Pacific salmon, genus Oncorhynchus. Pioneering experiments emphasized the importance of aerobic scope in setting the functional thermal tolerance for activity in fishes. Variation in routine metabolism can have important performance and fitness consequences as it is related to dominance, aggression, boldness, territoriality, growth rate, postprandial oxygen consumption, life history, season, time of day, availability of shelter and social interactions. Wild fishes must perform many activities simultaneously (e.g. swim, obtain prey, avoid predators, compete, digest and reproduce) and oxygen delivery is allocated among competing organ systems according to the capacity of the heart to deliver blood. For example, salmonids that are simultaneously swimming and digesting trade-off maximum swimming performance in order to support the oxygen demands of digestion. As adult Pacific salmonids cease feeding in the ocean prior to their home migration, endogenous energy reserves and cardiac capacity are primarily partitioned among the demands for swimming upriver, sexual maturation and spawning behaviours. Furthermore, the upriver spawning migration is under strong selection pressure, given that Pacific salmonids are semelparous (single opportunity to spawn). Consequently, these fishes optimize energy expenditures in a number of ways: strong homing, precise migration timing, choosing forward-assist current paths and exploiting the boundary layer to avoid the strong currents in the middle of the river, using energetically efficient swimming speeds, and recovering rapidly from anaerobic swimming. Upon arrival at the spawning ground, remaining energy can be strategically allocated to the various spawning behaviours. Strong fidelity to natal streams has resulted in reproductively isolated populations that appear to be locally adapted physiologically to their specific environmental conditions. Populations with more challenging migrations have enhanced cardiorespiratory performance. Pacific salmonids are able to maintain aerobic scope across the broad range of temperatures encountered historically during their migration; however, climate change-induced river warming has created lethal conditions for many populations, raising conservation concerns. Despite considerable research examining cardiorespiratory physiology in Pacific salmonids over the last 70 years, critical knowledge gaps are identified.
The HemoCue haemoglobin analyser consistently overestimated haemoglobin concentration ([Hb]) in the blood of all fish species (sockeye salmon Oncorhynchus nerka, Chinook salmon Oncorhynchus tshawytscha, Pacific bluefin tuna Thunnus orientalis and chub mackerel Scomber japonicus) by 22-50% (9Á9-36Á0 g l
This review examines selected areas of cardiovascular physiology where there have been impressive gains of knowledge and indicates fertile areas for future research. Because arterial blood is usually fully saturated with oxygen, increasing cardiac output is the only means for transferring substantially more oxygen to tissues. Consequently, any behavioural or environmental change that alters oxygen uptake typically involves a change in cardiac output, which in fishes can amount to a threefold change. During exercise, not all fishes necessarily have the same ability as salmonids to increase cardiac output by increasing stroke volume; they rely more on increases in heart rate instead. The benefits associated with increasing cardiac output via stroke volume or heart rate are unclear. Regardless, all fishes examined so far show an exquisite cardiac sensitivity to filling pressure and the cellular basis for this heightened cardiac stretch sensitivity in fish is being unraveled. Even so, a fully integrated picture of cardiovascular functioning in fishes is hampered by a dearth of studies on venous circulatory control. Potent positive cardiac inotropy involves stimulation of sarcolemmal β-adrenoceptors, which increases the peak trans-sarcolemmal current for calcium and the intracellular calcium transient available for binding to troponin C. However, adrenergic sensitivity is temperature-dependent in part through effects on membrane currents and receptor density. The membrane currents contributing to the pacemaker action potential are also being studied but remain a prime area for further study. Why maximum heart rate is limited to a low rate in most fishes compared with similar-sized mammals, even when Q10 effects are considered, remains a mystery. Fish hearts have up to three oxygen supply routes. The degree of coronary capillarization circulation is of primary importance to the compact myocardium, unlike the spongy myocardium, where venous oxygen partial pressure appears to be the critical factor in terms of oxygen delivery. Air-breathing fishes can boost the venous oxygen content and oxygen partial pressure by taking an air breath, thereby providing a third myocardial oxygen supply route that perhaps compensates for the potentially precarious supply to the spongy myocardium during hypoxia and exercise. In addition to venous hypoxemia, acidemia and hyperkalemia can accompany exhaustive exercise and acute warming, perhaps impairing the heart were it not for a cardiac protection mechanism afforded by β-adrenergic stimulation. With warming, however, a mismatch between an animal’s demand for oxygen (a Q10 effect) and the capacity of the circulatory and ventilatory systems to delivery this oxygen develops beyond an optimum temperature. At temperature extremes in salmon, it is proposed that detrimental changes in venous blood composition, coupled with a breakdown of the cardiac protective mechanism, is a potential mechanism to explain the decline in maximum and cardiac arrhythmias that are observed. Furthermore, the fall off in scope for heart rate and cardiac output is used to explain the decrease in aerobic scope above the optimum temperature, which may then explain the field observation that adult sockeye salmon ( Oncorhynchus nerka (Walbaum in Artedi, 1792)) have difficulty migrating to their spawning area at temperatures above their optimum. Such mechanistic linkages to lifetime fitness, whether they are cardiovascular or not, should assist with predictions in this era of global climate change.
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