CorrectionsBIOCHEMISTRY. For the article ''Interaction of RNA polymerase with forked DNA: Evidence for two kinetically significant intermediates on the pathway to the final complex,'' by Laura Tsujikawa, Oleg V. Tsodikov, and Pieter L. deHaseth, which appeared in number 6, March 19, 2002, of Proc. Natl. Acad. Sci. USA (99, 3493-3498; First Published March 12, 2002; 10.1073͞ pnas.062487299), the authors note the following concerning RNA polymerase (RNAP) concentrations. No correction was made for the fraction of RNAP (0.5) that is active in promoter binding. With this correction, the values of K 1 and K app (but not K f ) would increase by about a factor of 2. The relative values would remain essentially unchanged. Also, the legends to Figs. 2, 3, and 5 contain errors pertaining to the symbols used for data obtained with and without heparin challenge, the duration of the challenge, and the concentration of added heparin. The figures and the corrected legends appear below. Fig. 2. Determination of equilibrium affinities by titration of wt Fork with RNAP. The reactions contained 1 nM wt Fork and variable amounts of RNAP as shown and were analyzed by electrophoretic mobility shift immediately (OE; data shown are averages of three independent experiments) or after a challenge with 100 g͞ml heparin for 10 min (F; data shown are averages of four independent experiments). The curves shown reflect the simultaneous errorweighted fits of the data to Eqs. 3 and 4 -7. The parameters are shown in Table 1 (line 1). www.pnas.org͞cgi͞doi͞10.1073͞pnas.013667699 Fig. 3. Kinetics of complex formation. RNAP (65 nM) and wt forked DNA (1 nM) were incubated for various time intervals and then complex formation was determined immediately (Ϫheparin) or after a 2-min challenge with 100 g͞ml heparin (ϩheparin). The Ϫheparin data (s) were fit (error-weighted) with Eq. 8 with a 2 ϭ 0 (kaϪ ϭ 0.10 Ϯ 0.01 s Ϫ1 ) and the ϩheparin data (OE) with both single (k aϩ ϭ 0.036 Ϯ 0.004 s Ϫ1 ; thin line) and double-exponential (ka 1 ϭ 0.044 Ϯ 0.002 s Ϫ1 ; ka 2 ϭ (5 Ϯ 3) ϫ 10 Ϫ4 s Ϫ1 ; thick line) equations. Fig. 5.Comparison of the kinetics for formation and dissociation of competitor-resistant complexes between RNAP and wt Fork. Association data were obtained as described in the text and the legend for Fig. 3 except the concentration of forked DNA was 10 nM. Dissociation kinetics were obtained by challenging with 100 g͞ml heparin a mixture of RNAP and forked DNA that had been incubated for 30 min. The curves represent double-exponential fits of the data to Eq. 10. (A) wt RNAP. The observed association rate constants (s) are shown in the legend for Fig. 3; for the slow phase of the dissociation of the wt Fork-wt RNAP complex (F), kd 2 ϭ (1.3 Ϯ 0.2) ϫ 10 Ϫ4 s Ϫ1 . (B) YYW RNAP. The slow phase of the association reaction (F) has a ka 2 ϭ (1.1 Ϯ 0.3) ϫ 10 Ϫ3 s Ϫ1 ; the slow phase of the dissociation reaction (s), a kd 2 ϭ (6 Ϯ 1) ϫ 10 Ϫ4 s Ϫ1 . Fig. 6. BCL-6 preferentially binds to the wild-type exon 1 in Ly1 cells. Both Ly1 and the control Ly7 cells wer...
Developmentally regulated genes in Drosophila, which are conserved through evolution, are potential candidates for key functions in biological processes such as cell cycle, programmed cell death, and cancer. We report cloning and characterization of the human homologue of the Drosophila seven in absentia gene (HUMSIAH), which codes for a 282 amino acids putative zinc finger protein. HUMSIAH is localized on human chromosome 16ql2-ql3. This gene is activated during the physiological program of cell death in the intestinal epithelium. Moreover, human cancer-derived cells selected for suppression of their tumorigenic phenotype exhibit constitutively elevated levels of HUMSIAH mRNA. A similar pattern of expression is also displayed by the p2lwan. These results suggest that mammalian seven in absentia gene, which is a target for activation by p53, may play a role in apoptosis and tumor suppression.
The maps of the human immunoglobulin heavy-chain and kappa light-chain loci have recently been completed. We have now completed a map of the human lambda locus (IGL) located on chromosome 22q11.2. We mapped 52 V lambda genes from 10 V lambda families and 7 J lambda and C lambda genes on a 1140 kb contig constructed from eight YACs and 129 cosmid clones. The V lambda genes are arranged within 800 kb. Genes of the different V lambda families are organized in three clusters, V lambda II and III families (cluster A); V lambda I, V, VII and IX families (cluster B); V lambda IV, VI, VIII and X families (cluster C), in contrast to the dispersed organization of the different VH and V kappa families within the human VH and V kappa loci. We note that the most frequently used V lambda families (V lambda II and III) are proximal to the J lambda and C lambda genes. The VpreB gene, encoding part of the surrogate light chain, the GGT2 gene and the BCRL4 pseudogene were also mapped within the lambda locus.
Molecular study of a t(1;14)(p32;q11) translocation found in an acute T-cell leukemia (Kd cells) with a relatively mature phenotype is reported. Complex DNA rearrangements were characterized in the TCR alpha/delta locus. Besides a productive V alpha/J alpha assembly found on the normal allele, two deletions within the J alpha cluster were identified in the translocated allele. The translocation breakpoints involved the TCR delta gene on chromosome 14 and the SCL locus on chromosome band Ip32 that was recently shown to be activated by the t(1;14) translocation of the DU 528 leukemic cell line. Significantly, both Kd and DU 528 translocation breakpoints were located at the boundaries of D delta or J delta segments and were clustered in a 10 kb genomic fragment of the SCL gene. The presence of recombination signal motifs (heptamer-12/23 bp spacer-nonamer) on both normal chromosome partners, and N nucleotide addition on both derivative chromosomes involved the recombinase system in the translocation event. The SCL locus was highly expressed as a 5 kb transcript in Kd cells and, as already reported, as a 2 kb transcript in DU 528 cells. Importantly, a 5 kb SCL transcript was also detected in immature nonlymphoid hematopoietic cells but not in normal mature T cells, suggesting that it might correspond to the normal SCL transcript. Taken together, our data support the notion that the involvement of the SCL gene in the leukemogenic process may occur through overexpression of an apparently normal transcript (Kd cells) or expression of a truncated RNA (DU 528 cells).
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