Starting from 12,000 years ago in the Middle East, the Neolithic lifestyle spread across Europe via separate continental and Mediterranean routes. Genomes from early European farmers have shown a clear Near Eastern/Anatolian genetic affinity with limited contribution from hunter-gatherers. However, no genomic data are available from modern-day France, where both routes converged, as evidenced by a mosaic cultural pattern. Here, we present genome-wide data from 101 individuals from 12 sites covering today’s France and Germany from the Mesolithic (N = 3) to the Neolithic (N = 98) (7000–3000 BCE). Using the genetic substructure observed in European hunter-gatherers, we characterize diverse patterns of admixture in different regions, consistent with both routes of expansion. Early western European farmers show a higher proportion of distinctly western hunter-gatherer ancestry compared to central/southeastern farmers. Our data highlight the complexity of the biological interactions during the Neolithic expansion by revealing major regional variations.
Birch bark tar, the oldest adhesive known in Europe, was widely used during Prehistory. This material, produced by the dry distillation of birch bark, has been identified in various spheres of activities and provides valuable information on the know-how and technical and territorial systems of past societies. This biomaterial can also provide evidence on socio-economic networks and mobility. However, very little is known about the production systems of birch bark tar during Prehistory, including the Neolithic period. The lack of findings in the archaeological record necessitates the development of an approach that combines experimental archaeology and biomolecular chemistry. We present here (1) the results of experiments in which different birch bark tar production systems were tested and (2) the molecular signatures of the birch bark tars produced according to different processes based on the use of ceramic vessels. The key role of bark quality is highlighted for the first time. This study also details direct archaeological inference of the experimental results obtained: a total of 23 samples from the site of Nice-Giribaldi (France, second part of the fifth century BCE) was investigated. Different categories of birch bark tars were identified during Neolithic in the south of France, providing evidence for the existence of complex manufacturing systems and procurement networks.
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