A new regulator gene named pltZ, which is located downstream of the plt gene cluster in the genome of Pseudomonas sp. M18, was identified, sequenced and characterized in this report. The deduced amino acid sequence of PltZ shares significant homology with other bacterial regulators in the TetR family. The chromosomal pltZ disruption mutant gave rise to 4.4-fold enhancement of pyoluteorin biosynthesis but did not exert significant influence on the accumulation of phenazine-1-carboxylic acid compared with the wild-type M18. The negative regulation of pltZ on pyoluteorin biosynthesis was further confirmed by multiplied pltZ gene dosage experiments and pltAPPlacZ translational fusion analyses.
The pluripotent state is not solely governed by the action of the core transcription factors OCT4, SOX2, and NANOG, but also by a series of co-transcriptional and post-transcriptional events, including alternative splicing (AS) and the interaction of RNA-binding proteins (RBPs) with defined subpopulations of RNAs. Zinc Finger Protein 207 (ZFP207) is an essential transcription factor for mammalian embryonic development. Here, we employ multiple functional analyses to characterize its role in mouse embryonic stem cells (ESCs). We find that ZFP207 plays a pivotal role in ESC maintenance, and silencing of Zfp207 leads to severe neuroectodermal differentiation defects. In striking contrast to human ESCs, mouse ZFP207 does not transcriptionally regulate neuronal and stem cell-related genes but exerts its effects by controlling AS networks and by acting as an RBP. Our study expands the role of ZFP207 in maintaining ESC identity, and underscores the functional versatility of ZFP207 in regulating neural fate commitment.
Development
of versatile sensing methods for sensitive and specific
detection of clinically relevant nucleic acids and proteins is of
great value for disease monitoring and diagnosis. In this work, we
propose a novel isothermal Self-primer EXPonential Amplification Reaction
(SPEXPAR) strategy based on a rationally engineered structure-switchable
Metastable Hairpin template (MH-template). The MH-template initially
keeps inactive with its self-primer overhanging a part of target recognition
region to inhibit polymerization. The present targets can specifically
compel the MH-template to transform into an “activate”
conformation that primes a target-recyclable EXPAR. The method is
simple and sensitive, can accurately and facilely detect long-chain
single-stranded nucleic acids or proteins without the need of exogenous
primer probes, and has a high amplification efficiency theoretically
more than 2
n
. For a proof-of-concept demonstration,
the SPEXPAR method was used to sensitively detect the characteristic
sequence of the typical swine fever virus (CSFV) RNA and thrombin,
as nucleic acid and protein models, with limits of detection down
to 43 aM and 39 fM, respectively, and even the CSFV RNA in attenuated
vaccine samples and thrombin in diluted serum samples. The SPEXPAR
method may serve as a powerful technique for the biological research
of single-stranded nucleic acids and proteins.
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