Highlights d Two-component protein nanoparticles display multiple copies of the SARS-CoV-2 spike d Spike protein nanoparticles enhance cognate B cell activation in vitro d Vaccination induces potent neutralization in mice, rabbits, and cynomolgus macaques d Vaccination protects macaques against a high-dose SARS-CoV-2 challenge

Highlights d Two-component protein nanoparticles display multiple copies of the SARS-CoV-2 spike d Spike protein nanoparticles enhance cognate B cell activation in vitro d Vaccination induces potent neutralization in mice, rabbits, and cynomolgus macaques d Vaccination protects macaques against a high-dose SARS-CoV-2 challenge

The SARS-CoV-2 pandemic is continuing to disrupt personal lives, global healthcare systems and economies. Hence, there is an urgent need for a vaccine that prevents viral infection, transmission and disease. Here, we present a two-component protein-based nanoparticle vaccine that displays multiple copies of the SARS-CoV-2 spike protein. Immunization studies show that this vaccine induces potent neutralizing antibody responses in mice, rabbits and cynomolgus macaques. The vaccine-induced immunity protected macaques against a high dose challenge, resulting in strongly reduced viral infection and replication in upper and lower airways. These nanoparticles are a promising vaccine candidate to curtail the SARS-CoV-2 pandemic.