Amphibians are facing an extinction crisis, and conservation breeding programmes are a tool used to prevent imminent species extinctions. Compared to mammals and birds, amphibians are considered ideal candidates for these programmes due to their small body size and low space requirements, high fecundity, applicability of reproductive technologies, short generation time, lack of parental care, hard wired behaviour, low maintenance requirements, relative cost effectiveness of such programmes, the success of several amphibian conservation breeding programmes and because captive husbandry capacity exists. Superficially, these reasons appear sound and conservation breeding has improved the conservation status of several amphibian species, however it is impossible to make generalisations about the biology or geo-political context of an entire class. Many threatened amphibian species fail to meet criteria that are commonly cited as reasons why amphibians are suitable for conservation breeding programmes. There are also limitations associated with maintaining populations of amphibians in the zoo and private sectors, and these could potentially undermine the success of conservation breeding programmes and reintroductions. We recommend that species that have been assessed as high priorities for ex situ conservation action are subsequently individually reassessed to determine their suitability for inclusion in conservation breeding programmes. The limitations and risks of maintaining ex situ populations of amphibians need to be considered from the outset and, where possible, mitigated. This should improve programme success rates and ensure that the limited funds dedicated to ex situ amphibian conservation are allocated to projects which have the greatest chance of success.

Two strains, D5088T and D5095, representing a novel yeast species belonging to the genus Saccharomyces were isolated from oak tree bark and surrounding soil located at an altitude of 1000 m above sea level in Saint Auban, France. Sequence analyses of the internal transcribed spacer (ITS) region and 26S rRNA D1/D2 domains indicated that the two strains were most closely related to Saccharomyces mikatae and Saccharomyces paradoxus. Genetic hybridization analyses showed that both strains are reproductively isolated from all other Saccharomyces species and, therefore, represent a distinct biological species. The species name Saccharomyces jurei sp. nov. is proposed to accommodate these two strains, with D5088T (=CBS 14759T=NCYC 3947T) designated as the type strain.

The IUCN Red List of Threatened Species (Red List) details the extinction risk of the world's species and presents an important biodiversity indicator for conservation policy. Its continued utility relies on it containing up-to-date information on the extinction risk of species. This requires both regular reassessments and the timely assessment of newly described species. We provide an overview of the status of amphibian Red List assessments to highlight the difficulties of keeping assessments updated for species groups with high rates of species description. Since the publication of the IUCN's Global Amphibian Assessment in 2004, description rates of new species and assessment rates were initially similar; yet while the former has remained consistent, the latter has recently sharply declined. Currently 61.3% of amphibian species are either Not Evaluated or have out-of-date assessments. The situation is particularly problematic in countries with the richest amphibian diversity, which typically have the highest rates of amphibian species discovery and face the greatest threats. Efforts to keep the Red List up-to-date are primarily limited by funding, we estimate that an annual investment of US $170,478−$319,290 is needed to have an up-todate Red List for amphibians. We propose suggestions to increase assessment rates by improving the availability of data relevant to the process: authors of species descriptions or taxonomic revisions should publish information relevant to Red List assessments. Taxonomic journals should suggest inclusion of such information in their author guidelines. We suggest that contributors with significant input into assessments should be rewarded with co-authorship of published assessments. Keywords Amphibian, conservation prioritization, Global Amphibian Assessment, IUCN Red List of Threatened Species, extinction risk. Introduction The IUCN Red List of Threatened Species (hereafter the 'Red List') is a centralised, widely accepted measure of global extinction risk used to identify threats and priority conservation actions (Lamoreux et al. 2003; Rondinini et al. 2014). The Red List tracks changes in extinction risk over time, and is important in measuring threats to biodiversity and evaluating the impact of conservation intervention on a global scale (Hoffmann et al. 2010). The Red List has significant influence over which research and conservation work is resourced as grant funding often prioritizes globally threatened species. It also underpins the Red List Index, an important biodiversity indicator steering conservation policy (Butchart et al. 2004; Butchart et al. 2007; Butchart et al. 2010). The Red List is currently supported by fund-raising efforts carried out by the IUCN and Red List Partners, and through philanthropy (Rondinini et al. 2014; Juffe-Bignoli et al. 2016). However, once a taxonomic group has been comprehensively assessed, funding for subsequent assessments is not readily available (Rondinini et al. 2014). Much of the work required to assess a species, particularly provid...

Despite the importance of ex situ conservation programmes as highlighted in the Amphibian Conservation Action Plan, there are few empirical studies that examine the influence of captive conditions on the fitness of amphibians, even for basic components of enclosure design such as cover provision. Maintaining the fitness of captive amphibian populations is essential to the success of ex situ conservation projects. Here we examined the impact of plant cover on measures of fitness and behaviour in captive red-eyed tree frogs (Agalychnis callidryas). We found significant effects of plant provision on body size, growth rates and cutaneous bacterial communities that together demonstrate a compelling fitness benefit from cover provision. We also demonstrate a strong behavioural preference for planted rather than non-planted areas. We also assessed the impact of plant provision on the abiotic environment in the enclosure as a potential driver of these behavioural and fitness effects. Together this data provides valuable information regarding enclosure design for a non-model amphibian species and has implications for amphibian populations maintained in captivity for conservation breeding programmes and research.

Many amphibian species are dependent on ex situ conservation interventions for their long-term persistence. However, projects have been jeopardised by husbandry issues involving poor calcium metabolism and nutritional metabolic bone disease (NMBD). Healthy calcium metabolism requires appropriate dietary calcium content and access to vitamin D3 . In many animals, vitamin D3 can be photobiosynthesised in skin exposed to UVB radiation, as well as extracted from the diet, but the extent of vitamin D3 photobiosynthesis in amphibians is poorly known. Additionally, prey insects for captive amphibians are deficient in calcium and calcium content must be artificially increased, but the effects of different levels of augmentation and their interaction with UVB exposure are also little understood. We fed captive fire-bellied toads (Bombina orientalis) with crickets augmented to contain 5% and 10% calcium and housed them with and without UVB exposure. Despite additional dietary vitamin D3 supplementation, we found that toads exposed to UVB radiation exhibited significantly higher serum vitamin D3 levels, indicating that this species may partly rely on photobiosynthesis sources of vitamin D3 . These data are the first to show a direct link between UVB exposure and serum vitamin D3 in an amphibian. We found significant positive effects of UVB exposure and 10% dietary calcium content on skeletal structure, as well as complex interactions between treatments. We also found UVB radiation exposure resulted in more rapid natural coloration acquisition. Together, this indicates that standard calcium plus vitamin D3 supplementation methods may not fully substitute for UVB exposure and for increased feeder insect calcium content. This may have implications for the success of ex situ amphibian conservation, as well as for the welfare of captive amphibians in general. Our data lend support for the provision of UVB radiation for captive, basking amphibians.