Rural environment is suffering from serious problems, as reflected in the term “Empty Spain”. One of these problems is the pronounced depopulation that rural areas suffer in our time, so creating links with the land thanks to education is of great interest for, among other things, establishing population in rural areas. Interdisciplinary education becomes relevant today as the necessary education in our current world capable of providing answers and solutions to the social demands of our time. Interdisciplinary STEM education had the United States of America as its cradle in the 1990s; later it passed to the acronym STEAM when the Arts were later introduced, this is how you find a true interdisciplinary education. Since 2010, government policies have been developed in the USA, highlighting the Educate to Innovate program and in that same country the STEM4SD Education program, which develops education for sustainability by creating links with the local population. Precisely, this article will collect the educational policies that have been carried out in the USA for the development of this type of education. In this article and thanks to the analysis of certain programs, the importance of interdisciplinary STEM and STEAM education in our days will be exposed for the promotion of sustainability directed towards sustainable development, thereby creating more sustainable societies made up of more sustainable citizens, highlighting the importance of education for sustainability through STEM and STEAM education creating links with the land for the improvement of the rural world, which means establishing population, among other aspects.
Among the biotechnological uses of plant viruses, the expression of foreign sequences through virus‐based vectors represents a promising research area. The potyvirus Plum pox virus (PPV) has been used to design expression vectors which have allowed successful expression of foreign sequences in plants, either in the form of small peptides fused to the viral coat protein, or as whole independent proteins inserted on different points of the genome. The present review describes the different PPV‐based vectors that have been produced, including information regarding relevant aspects of their use, such as the optimal location of peptides or the stability of inserts. Recent developments, like the expression of proteins on stone fruit trees by using PPV‐based vectors capable to infect woody plants, are also described.
BackgroundPlant genomes have been transformed with full-length cDNA copies of viral genomes, giving rise to what has been called 'amplicon' systems, trying to combine the genetic stability of transgenic plants with the elevated replication rate of plant viruses. However, amplicons' performance has been very variable regardless of the virus on which they are based. This has boosted further interest in understanding the underlying mechanisms that cause this behavior differences, and in developing strategies to control amplicon expression.ResultsNicotiana benthamiana plants were transformed with an amplicon consisting of a full-length cDNA of the potyvirus Plum pox virus (PPV) genome modified to include a GFP reporter gene. Amplicon expression exhibited a great variability among different transgenic lines and even among different plants of the same line. Plants of the line 10.6 initially developed without signs of amplicon expression, but at different times some of them started to display sporadic infection foci in leaves approaching maturity. The infection progressed systemically, but at later times the infected plants recovered and returned to an amplicon-inactive state. The failure to detect virus-specific siRNAs in 10.6 plants before amplicon induction and after recovery suggested that a strong amplicon-specific RNA silencing is not established in these plants. However, the coexpression of extra viral silencing suppressors caused some amplicon activation, suggesting that a low level of RNA silencing could be contributing to maintain amplicon repression in the 10.6 plants. The resistance mechanisms that prevent amplicon-derived virus infection were also active against exogenous PPV introduced by mechanical inoculation or grafting, but did not affect other viruses. Amplicon-derived PPV was able to spread into wild type scions grafted in 10.6 rootstocks that did not display signs of amplicon expression, suggesting that resistance has little effect on virus movement.ConclusionsOur results suggest that amplicon-derived virus infection is limited in this particular transgenic line by a combination of factors, including the presumed low efficiency of the conversion from the transgene transcript to replicable viral RNA, and also by the activation of RNA silencing and other defensive responses of the plant, which are not completely neutralized by viral suppressors.
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