Highlights d Dimedone Switch method is a versatile, chemoselective persulfide labeling approach d Protein persulfidation is an evolutionarily conserved modification of cysteine thiols d Persulfidation waves rescue cysteines from overoxidation caused by ROS d Persulfidation decreases with aging, increases with caloric restriction, and extends lifespan

As a result of an author oversight in the originally published version of this article, the surname of author Bruno Gonzalez-Zorn was misspelled as ''Gonzales-Zorn.'' Additionally, the scheme in the Graphical Abstract contains a final product of proteinS -Sdimedone, rather than proteinS -dimedone. These errors have now been corrected in the article online. The authors apologize for the errors and any inconvenience that may have resulted.

Highlights d MYC enhances productive transcription by defining the protein composition of Pol II d MYC directly binds SPT5 and hands it over to Pol II in a CDK7dependent manner d Transfer of SPT5 increases speed and processivity of Pol II d MYC's effects on Pol II function shape its tumor-specific gene expression profile