PurposeTo assess Cyclosporine A (CsA) therapy at an intraperitoneal dose of 15 mg.kg
-1 in a rodent model of non-septic renal ischemia.MethodsTwenty male Wistar rats were randomized to receive CsA therapy or none
therapy before undergoing 30 minutes of renal ischemia followed by
reperfusion. Additionally, 10 rats were randomized to undergo the same
surgical procedure of the aforementioned animals with neither ischemia nor
CsA therapy. Twelve hours after kidney ischemia, the left kidneys were
evaluated for histological injury according to Park’s criteria. Serum
creatinine (Cr), urea nitrogen (Ur) and sodium levels were obtained at
different times of the experimental protocol.ResultsRodents in the CsA group showed negative results (p<0.05) in serum
variables (Cr: 0.41±0.05mg/dL vs . 4.17±1.25mg/dL; Ur:
40.90±3.98mg/dL vs . 187.70±22.93mg/dL) even the non CsA or
control group (Cr: 0.35±0.07mg/dL vs . 3.80±1.20mg/dL; Ur:
40.10±4.70mg/dL vs . 184.50±49.80mg/dL). The negative
results were also verified in histological evaluation, CsA group had 50% in
the very severe grade of lesion, 10% in the severe and 40% in the moderate
to severe whereas the control group had 90% in the very severe grade.ConclusionCsA was incapable of preventing the deleterious effects of
ischemia-reperfusion injury in rat kidneys.
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