Background and Objectives: The basis of any infection control program is hand hygiene (HH). The aim of this study was to investigate knowledge of HH among medical students. Materials and Methods: Students were randomly selected from two Romanian universities and a cross-sectional, questionnaire-based study was conducted between January and May 2021. The answers regarding demographic data and knowledge concerning the methods, the time and the antiseptics used for HH were collected. The selection of the study group was made according to selection criteria in accordance with ethical issues. A descriptive statistical analysis was performed, and a chi-square test was used for data comparison, with a cut-off point of 0.05 for statistical significance. Results: The results indicated that the attitude of the students towards the practice of HH improved significantly. Most students believe that simple HH can control infections. Significant differences were found by the year of study in terms of the hand surfaces included and recommended duration (p < 0.05). Conclusions: In conclusion, the study shows that most respondents have sufficient knowledge on HH, meaning that a higher compliance is required to control infections. The indicated reasons of non-compliance with HH are emergencies and other priorities.

This study evaluated the effects of ortophosforic acid and Er.Cr: YSGG LASER on human enamel by means of a field emission in-lens scanning electron microscope. The materials used for conditioning the teeth were SE�35% (Scotchbond� Etchant Phosphoric Acid) and MG6-MZ6 tips with Er, Cr: YSGG laser, 30% water, 60% air, 5.5 W/20 Hz according to manufacturer�s instructions. The acid time of action determines enamel demineralization in all situation. Er.Cr: YSGG laser treatment of non-carious enamel, a laser power of 5.5 W is found to be the optimal to improve the micromorphology of enamel.

Background and Objectives: the purpose of this study was to evaluate students’ level of knowledge and attitude towards oral hygiene. Materials and Methods: the evaluation was carried out by a questionnaire, with 30 Q (questions) as follows: demographic data (Q1–Q5), oral hygiene knowledge data (Q6–Q23) and oral hygiene attitude data (Q24–Q30). The study included students from Romanian schools and the selection of the study group was made following selection criteria in accordance with ethical issues. A descriptive statistical analysis was performed and a value of p ≤ 0.05 was considered statistically significant. Results: the study included a number of 718 subjects with a mean age of 14.54 (±2.22), male 250 (34.8%) and female 468 (65.2%), MS (middle school students) 354 (49.4%) and HH (high school students) 364 (50.6%). Most of the subjects 292 (MS = 160; HS = 132) know a toothbrushing technique, p = 0.009, r = 0.091 and 587 (MS = 278; HS = 309) know that brushing removes the bacterial plaque p = 0.027, r = −0.082 but only 147 (MS = 71; HS = 76) know that (by) brushing can re-mineralize hard dental structures. The duration of the toothbrushing is variable, for 2- or 3-min p = 0.058, r = 0.043. Criteria for choosing the toothbrush were based mainly on the indications of the dentist, respectively, for toothpaste on its properties. The frequency of toothbrushing is mainly twice a day 428 (MS = 234; HS = 248), p = 0.079, r = 0.037, 73 (MS = 33; HS = 40) after every meal. p = 0.099, r = 0.095. Mouthwash is used by 421 (MS = 199; HS = 222) p = 0.111, r = −0.048, and 228 (MS = 199; HS = 222) after each brushing. Dental floss is used by 240 (MS = 106; HS = 134), p = 0.031, r = −0.073 and only 74 (MS = 41; HS = 33) after each brushing. Conclusions: there are differences in the level of knowledge and attitudes regarding the determinants of oral hygiene depending on the level of education.

(1) Background: Numerous studies have shown the beneficial role of fluoride in the primary prevention of dental caries. The aim of the present study was to put into evidence the change in the enamel structure immediately after the application of a fluoride varnish. (2) Methods: A xylitol–fluoride varnish was evaluated. The enamel specimens (n = 10) were analyzed by atomic force microscopy on enamel surface and treatment with fluoride varnish applied. The dimensional topographic analysis was performed by 2D and 3D analysis software. Statistical analysis was performed using SPSS Version 26.00 (IBM, Armonk, NY, USA). A one-sample statistics test was used to identify significant differences (p < 0.05). (3) Results: Surface roughness (Ra) measurements ranged from Ra = 0.039 μm (±0.048), to Ra = 0.049 μm (±0.031), respectively (p < 0.05), with an increase in the surface roughness passing from the intact enamel to the enamel exposed to fluoride varnish. When comparing Ra values of the nonfluorinated enamel and fluorinated enamel, significant differences (p < 0.05) were found, suggesting that the varnish had a protective effect on the enamel surface. (4) Conclusions: Xylitol–fluoride varnish, even in one single short-time application, is effective in reducing the surface roughness of enamel structure exposed to abrasion, thus increasing resistance to dental caries.

Background and Objectives: The HH (hand hygiene) technique is relatively simple. Even so, in practice there are still non-conformities regarding this aspect. Lack of knowledge or lack of compliance can be reasons for non-adherence with HH techniques. In this context, the purpose of this study was to follow the realization of the hand-washing technique with hydroalcoholic solution, applied before and after receiving the HH recommendations. Materials and Methods: A descriptive, cross-sectional study was conducted from May 2022 to June 2022. Students from a second year dental medicine class teaching in French 2021/22 “Grigore T. Popa” University of Medicine and Pharmacy in Iasi were included in the study. The approval of the ethics commission was received: No. 184/05.05.2022. The study was conducted in two stages. In the first stage, HH was performed without any recommendation. In the second stage, the antiseptic hand rubbing technique was presented following the WHO recommendations. The fluorescent Fluo-Add solution, Wood lamp for dermatology (4 × 5.5 W ultraviolet tubes with a wavelength of 360 nm), and photo camera were used. Subjects performed their HH movement before and after receiving instructions according to WHO. Images were initially taken from the backhand and palm and were finally analyzed with Image Color Summarizer. The data were analyzed by the Mann–Whitney U Test, t-test paired samples using IBM-SPSS version 26 (IBM, Armonk, NY, USA), and p ≤ 0.05 was considered statistically significant. Results: After analyzing the images, there were 70 subjects, 45.7% (32) female and 54.33% (38) male. Final average covered area of backhand was 60.89% (±17.17), 28.84:86.11, compared to 52.07% (±17.04), 9:85.23. Final average covered area for palm was 69.91% (±13.5), 31.61:93.41 compared to 59.74% (±16.64), 26.13:92.72. No statistical significance was obtained by gender. Conclusions: The study showed an improvement in hand hygiene technique without highlighting gender differences.