On 2017 August 17 a binary neutron star coalescence candidate (later designated GW170817) with merger time 12:41:04 UTC was observed through gravitational waves by the Advanced LIGO and Advanced Virgo detectors. The Fermi Gamma-ray Burst Monitor independently detected a gamma-ray burst (GRB 170817A) with a time delay of ∼ 1.7 s with respect to the merger time. From the gravitational-wave signal, the source was initially localized to a sky region of 31 deg2 at a luminosity distance of 40 − 8 + 8 Mpc and with component masses consistent with neutron stars. The component masses were later measured to be in the range 0.86 to 2.26 M ⊙ . An extensive observing campaign was launched across the electromagnetic spectrum leading to the discovery of a bright optical transient (SSS17a, now with the IAU identification of AT 2017gfo) in NGC 4993 (at ∼ 40 Mpc ) less than 11 hours after the merger by the One-Meter, Two Hemisphere (1M2H) team using the 1 m Swope Telescope. The optical transient was independently detected by multiple teams within an hour. Subsequent observations targeted the object and its environment. Early ultraviolet observations revealed a blue transient that faded within 48 hours. Optical and infrared observations showed a redward evolution over ∼10 days. Following early non-detections, X-ray and radio emission were discovered at the transient’s position ∼ 9 and ∼ 16 days, respectively, after the merger. Both the X-ray and radio emission likely arise from a physical process that is distinct from the one that generates the UV/optical/near-infrared emission. No ultra-high-energy gamma-rays and no neutrino candidates consistent with the source were found in follow-up searches. These observations support the hypothesis that GW170817 was produced by the merger of two neutron stars in NGC 4993 followed by a short gamma-ray burst (GRB 170817A) and a kilonova/macronova powered by the radioactive decay of r-process nuclei synthesized in the ejecta.

The dwarf spheroidal satellite galaxies (dSphs) of the Milky Way are some of the most dark matter (DM) dominated objects known. We report on γ-ray observations of Milky Way dSphs based on six years of Fermi Large Area Telescope data processed with the new Pass8 event-level analysis. None of the dSphs are significantly detected in γ rays, and we present upper limits on the DM annihilation cross section from a combined analysis of 15 dSphs. These constraints are among the strongest and most robust to date and lie below the canonical thermal relic cross section for DM of mass ≲100  GeV annihilating via quark and τ-lepton channels.

ForewordThe Pierre Auger Observatory has begun a major Upgrade of its already impressive capabilities, with an emphasis on improved mass composition determination using the surface detectors of the Observatory. Known as AugerPrime, the upgrade will include new 4 m 2 plastic scintillator detectors on top of all 1660 water-Cherenkov detectors, updated and more flexible surface detector electronics, a large array of buried muon detectors, and an extended duty cycle for operations of the fluorescence detectors.This Preliminary Design Report was produced by the Collaboration in April 2015 as an internal document and information for funding agencies. It outlines the scientific and technical case for AugerPrime 1 . We now release it to the public via the arXiv server. We invite you to review the large number of fundamental results already achieved by the Observatory and our plans for the future.The Pierre Auger Collaboration 1 As a result of continuing R&D, slight changes have been implemented in the baseline design since this Report was written. These changes will be documented in a forthcoming Technical Design Report. ix x Executive Summary Present Results from the Pierre Auger ObservatoryMeasurements of the Auger Observatory have dramatically advanced our understanding of ultra-high energy cosmic rays. The suppression of the flux around 5×10 19 eV is now confirmed without any doubt. Strong limits have been placed on the photon and neutrino components of the flux indicating that "top-down" source processes, such as the decay of superheavy particles, cannot account for a significant part of the observed particle flux. A largescale dipole anisotropy of ∼7% amplitude has been found for energies above 8×10 18 eV. In addition there is also an indication of the presence of a large scale anisotropy below the ankle. Particularly exciting is the observed behavior of the depth of shower maximum with energy, which changes in an unexpected, non-trivial way. Around 3×10 18 eV it shows a distinct change of slope with energy, and the shower-to-shower variance decreases. Interpreted with the leading LHC-tuned shower models, this implies a gradual shift to a heavier composition. A number of fundamentally different astrophysical model scenarios have been developed to describe this evolution. The high degree of isotropy observed in numerous tests of the small-scale angular distribution of UHECR above 4×10 19 eV is remarkable, challenging original expectations that assumed only a few cosmic ray sources with a light composition at the highest energies. Interestingly, the largest departures from isotropy are observed for cosmic rays with E > 5.8×10 19 eV in ∼20 • sky-windows. Due to a duty cycle of ∼15% of the fluorescence telescopes, the data on the depth of shower maximum extend only up to the flux suppression region, i.e. 4×10 19 eV. Obtaining more information on the composition of cosmic rays at higher energies will provide crucial means to discriminate between the model classes and to understand the origin of the observed flux suppre...

The metabolism of aerobic organisms continuously produces reactive oxygen species. Although potentially toxic, these compounds also function in signaling. One important feature of signaling compounds is their ability to move between different compartments, e.g. to cross membranes. Here we present evidence that aquaporins can channel hydrogen peroxide (H 2 O 2 ). Twenty-four aquaporins from plants and mammals were screened in five yeast strains differing in sensitivity toward oxidative stress. Expression of human AQP8 and plant Arabidopsis TIP1;1 and TIP1;2 in yeast decreased growth and survival in the presence of H 2 O 2 . Further evidence for aquaporin-mediated H 2 O 2 diffusion was obtained by a fluorescence assay with intact yeast cells using an intracellular reactive oxygen species-sensitive fluorescent dye. Application of silver ions (Ag ؉ ), which block aquaporin-mediated water diffusion in a fast kinetics swelling assay, also reversed both the aquaporindependent growth repression and the H 2 O 2 -induced fluorescence. Our results present the first molecular genetic evidence for the diffusion of H 2 O 2 through specific members of the aquaporin family.Hydrogen peroxide (H 2 O 2 ) 2 belongs to the group of reactive oxygen species (ROS). ROS are generated in a number of key metabolic processes in cells like the electron transport chain in the inner mitochondrial membrane (1) and, specific for plants, the chloroplast thylakoid membrane (2).Because ROS can potentially damage proteins, lipids, and nucleic acids, cells have a number of ROS-scavenging systems that are able to remove these molecules and to maintain a relatively low and constant ROS concentration (3). However, ROS are also intermediates in various signal transduction pathways and have been shown to initiate responses to various stresses and disorders (for recent reviews, see Refs. 4 and 5). Arabidopsis mutants lacking an NADPH oxidase were not able to respond adequately to potassium deficiency (6) and were impaired in stomatal closure (7), providing genetic evidence for a role of NADPH oxidase in signaling.ROS are interconvertible molecules including singlet oxygen, superoxide, hydroxyl radical, and H 2 O 2 . H 2 O 2 has a distinctive set of features compared with other ROS. (i) It is not charged, (ii) it is not a radical, (iii) it possesses an intermediate oxidation number, (iv) it is relatively stable under physiological conditions, and (v) catalase can disproportionate it into water and molecular oxygen without the expense of reduction equivalents.Although substantial progress has been made regarding the formation and scavenging of ROS, little is known about their transport from the site of origin to the place of action or detoxification. Recently three studies from mammalian systems have provided evidence that H 2 O 2 , in addition to the well studied role in intracellular signaling, is also used as an intercellular signal molecule (8 -10). This implies that a necessary step within these signal transduction pathways is the transport of H 2 O 2 ...

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104 3Dark matter in the Milky Way may annihilate directly into γ rays, producing a monoenergetic 105 spectral line. Therefore, detecting such a signature would be strong evidence for dark matter from the modified observing strategy designed to increase exposure of the Galactic center region. 111We searched in five sky regions selected to optimize sensitivity to different theoretically-motivated 112 dark matter scenarios and find no significant detections. In addition to presenting the results from 113 our search for lines, we also investigate the previously reported tentative detection of a line at 133 114 GeV using the new Pass 8 data.115

Regulation of the trans-plasma membrane pH gradient is an important part of plant responses to several hormonal and environmental cues, including auxin, blue light, and fungal elicitors. However, little is known about the signaling components that mediate this regulation. Here, we report that an Arabidopsis thaliana Ser/Thr protein kinase, PKS5, is a negative regulator of the plasma membrane proton pump (PM H+ -ATPase). Loss-of-function pks5 mutant plants are more tolerant of high external pH due to extrusion of protons to the extracellular space. PKS5 phosphorylates the PM H+ -ATPase AHA2 at a novel site, Ser-931, in the C-terminal regulatory domain. Phosphorylation at this site inhibits interaction between the PM H+ -ATPase and an activating 14-3-3 protein in a yeast expression system. We show that PKS5 interacts with the calcium binding protein SCaBP1 and that high external pH can trigger an increase in the concentration of cytosolic-free calcium. These results suggest that PKS5 is part of a calcium-signaling pathway mediating PM H+ -ATPase regulation.

Gamma-ray line signatures can be expected in the very-high-energy (E(γ)>100 GeV) domain due to self-annihilation or decay of dark matter (DM) particles in space. Such a signal would be readily distinguishable from astrophysical γ-ray sources that in most cases produce continuous spectra that span over several orders of magnitude in energy. Using data collected with the H.E.S.S. γ-ray instrument, upper limits on linelike emission are obtained in the energy range between ∼ 500 GeV and ∼ 25 TeV for the central part of the Milky Way halo and for extragalactic observations, complementing recent limits obtained with the Fermi-LAT instrument at lower energies. No statistically significant signal could be found. For monochromatic γ-ray line emission, flux limits of (2 × 10(-7) -2 × 10(-5)) m(-2) s(-1) sr(-1) and (1 × 10(-8) -2 × 10(-6)) m(-2) s(-1)sr(-1) are obtained for the central part of the Milky Way halo and extragalactic observations, respectively. For a DM particle mass of 1 TeV, limits on the velocity-averaged DM annihilation cross section ⟨σv⟩(χχ → γγ) reach ∼ 10(-27) cm(3)s(-1), based on the Einasto parametrization of the Galactic DM halo density profile.