Gastric cancer is a heterogeneous disease with multiple environmental etiologies and alternative pathways of carcinogenesis. Beyond mutations in TP53, alterations in other genes or pathways account for only small subsets of the disease. We performed exome sequencing of 22 gastric cancer samples and identified previously unreported mutated genes and pathway alterations; in particular, we found genes involved in chromatin modification to be commonly mutated. A downstream validation study confirmed frequent inactivating mutations or protein deficiency of ARID1A, which encodes a member of the SWI-SNF chromatin remodeling family, in 83% of gastric cancers with microsatellite instability (MSI), 73% of those with Epstein-Barr virus (EBV) infection and 11% of those that were not infected with EBV and microsatellite stable (MSS). The mutation spectrum for ARID1A differs between molecular subtypes of gastric cancer, and mutation prevalence is negatively associated with mutations in TP53. Clinically, ARID1A alterations were associated with better prognosis in a stage-independent manner. These results reveal the genomic landscape, and highlight the importance of chromatin remodeling, in the molecular taxonomy of gastric cancer.
Highlights d Living biobank includes 17 normal and 46 gastric cancer organoid lines d Organoid biobank encompasses most of the known molecular subtypes of gastric cancer d Organoids recapitulate the genomic and transcriptomic features of original tumors d High-throughput screen revealed potential target drugs for personalized therapy
Gastric cancer is the world's second most common cause of cancer death. We
analyzed gene expression patterns in 90 primary gastric cancers, 14 metastatic
gastric cancers, and 22 nonneoplastic gastric tissues, using cDNA microarrays
representing ∼30,300 genes. Gastric cancers were distinguished from
nonneoplastic gastric tissues by characteristic differences in their gene
expression patterns. We found a diversity of gene expression patterns in
gastric cancer, reflecting variation in intrinsic properties of tumor and
normal cells and variation in the cellular composition of these complex
tissues. We identified several genes whose expression levels were
significantly correlated with patient survival. The variations in gene
expression patterns among cancers in different patients suggest differences in
pathogenetic pathways and potential therapeutic strategies
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