Chemotaxonomic and 16S rRNA gene sequence analyses of an isolate from the bronchial secretions of a patient with chronic bronchitis demonstrated clearly that it belongs to the genus Nocardia. The 16S rRNA gene sequence data, as well as the biochemical characteristics of the isolate, indicated that it belongs to a new species that differs from previously described members of the genus Nocardia. The name Nocardia cyriacigeorgici sp. nov. is proposed for this isolate, and is represented by strain IMMIB D-1627 T (l DSM 44484 T ).
A new species of the genus Nocardiopsis, for which we propose the name Nocardiopsis lucentensis sp. nov. (type strain, strain DSM 44048), was isolated from a salt marsh soil sample near Alicante, Spain. Whole-cell hydrolysates contain the meso isomer of diaminopimelic acid and no characteristic sugar; thus, the cell wall composition is type 111. Menaquinone MK-lO(H,) is the major menaquinone, and the phospholipid type is type PI11 (phosphatidylcholine present). Spore chains are rectiflexibilis, and in the early stages of sporulation zig-zag-shaped aerial hyphae are observed. This microorganism produces compatible solutes of the ectoine type and is characterized by a yellowish to yellowish brown substrate mycelium and a white aerial mycelium. This organism exhibits only 40 to 50% DNA relatedness to other Nocardiopsis spp.
Chemotaxonomic and 16s ribosomal DNA sequence analyses of an isolate from the sputum of a patient with a mycobacterial lung infection clearly delineated a new species of the genus Tsukarnurella. This new species can be defined on the basis of genotypic and phenotypic data. The name Tsukamurella pulmonis sp. nov. is proposed for this organism; the type strain is IMMIB D-1321T (= DSM 44142T). This isolate shows 44.2 and 36.2% DNA relatedness to Tsukamurella paurometabola DSM 20162T (T = type strain) and Tsukamurella inchonensis DSM 44067T, respectively.
Chemotaxonomic and genomic 16s ribosomal DNA sequence analyses of two isolates obtained from two different clinical materials clearly delineated a new species of the genus TsukamureZlu. This new species can be identified by its 16s ribosomal DNA similarity values, as well as its physiological characteristics. The name TsukamureZh inchonensis sp. nov. is proposed for these isolates, which are represented by strain IMMIB D-771T (= DSM 44067T) (T = type strain). This strain exhibits only 45% DNA relatedness to Tsukamurella pauro-
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