Hispolon suppresses migration and invasion of human nasopharyngeal carcinoma cells by inhibiting the urokinase‐plasminogen activator through modulation of the Akt signaling pathway

Ho, Hsin‐Yu; Ho, Yung‐Chuan; Hsieh, Ming‐Ju; Yang, Shun‐Fa; Chuang, Chun‐Yi; Hsin, Chung‐Han

doi:10.1002/tox.22266

Cited by 32 publications

(34 citation statements)

References 41 publications

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“…Natural substances, including traditional Chinese medications, greatly contribute to the blockage of tumour metastasis . Rheum palmatum L., a traditional Chinese medication, has been used for the treatment of many diseases such as constipation, jaundice, ulcers, and gastrointestinal hemorrhage .…”

Section: Introductionmentioning

confidence: 99%

Antimetastatic effects ofRheum palmatumL. extract on oral cancer cells

Chen

Hsieh

et al. 2017

Environmental Toxicology

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Rheum palmatum L., a traditional Chinese medication, has been used for the treatment of various disorders. However, the detailed impacts and underlying mechanisms of R. palmatum L. extracts (RLEs) on human oral cancer cell metastasis are still unclear. Here, we tested the hypothesis that an RLE has antimetastatic effects on SCC-9 and SAS human oral cancer cells. Gelatin zymography, Western blot, real-time polymerase chain reaction, and luciferase assay were used to explore the underlying mechanisms involved in the antimetastatic effects on oral cancer cells. Our results revealed that the RLE (up to 20 μg/mL, without cytotoxicity) attenuated SCC-9 and SAS cell motility, invasiveness, and migration by reducing matrix metalloproteinase (MMP)-2 enzyme activities. Western blot analysis of the MAPK signaling pathway indicated that the RLE significantly decreased phosphorylated ERK1/2 levels but not p38 and JNK levels. In conclusion, RLEs exhibit antimetastatic activity against oral cancer cells through the transcriptional repression of MMP-2 via the Erk1/2 signaling pathways. Thus, RLEs may be potentially useful as antimetastatic agents for oral cancer chemotherapy.

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Section: Introductionmentioning

confidence: 99%

Antimetastatic effects ofRheum palmatumL. extract on oral cancer cells

Chen

Hsieh

et al. 2017

Environmental Toxicology

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“…Cell migration and invasion was assayed according to the methods described previously [25,26]. After treatment with PSH for 24 h, the surviving SCC-9, SAS and HSC-3 cells were harvested and seeded to upper Transwell (Greiner Bio-One, North Carolina, USA), and DMEM-F12 medium supplemented with 10% FBS (600 µl) was added in advance into Transwell lower chambers in a 24-well plate, then incubated for 24 h at 37°C.…”

Section: Cell Migration and Invasion Assaysmentioning

confidence: 99%

Pinostilbene Hydrate Suppresses Human Oral Cancer Cell Metastasis by Downregulation of Matrix Metalloproteinase-2 Through the Mitogen-Activated Protein Kinase Signaling Pathway

Hsieh

Chin

Lin

et al. 2018

Cell Physiol Biochem

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Background/Aims: Cancer is the most common cause of death worldwide with approximately one third of people being diagnosed with cancer in their lifetime. Pinostilbene hydrate (PSH) A methylated derivative of resveratrol Has been reported to possess antioxidative Cardioprotective and anticancer properties. However the antimetastatic effect of pinostilbene in oral squamous cell carcinoma (OSCC) remains unknown. Methods: In this study We investigated the effect of PSH on antimetastatic activity and the relevant signaling pathways underlying mechanisms of SCC-9 SAS and HSC-3 oral cancer cell lines by MTT assay Wound healing Transwell assay Zymography and western blot analysis. Results: Our findings indicated that PSH inhibits migration and invasion ability by reducing the protein activity and expression of matrix metalloproteinases-2 (MMP-2) in all three cell lines. Moreover • The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinases (p38) had significant inhibitory effects in the presence of PSH in the SCC9 and SAS cell lines. A combination of ERK1/2 and p38 inhibitors with PSH also reduced the migration and activity of MMP-2 in the SCC9 and SAS cell lines. Conclusion: This study demonstrated that PSH suppresses MMP-2 enzymatic activity by downregulating the p38/ERK1/2 pathway and that it might be a promising agent for preventing OSCC cell metastasis.

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“…Herbal compositions from natural compounds have been utilized in preclinical or clinical trials with chemopreventive or therapeutic drugs for cancer management . Although chemotherapy and targeted therapy are effective methods for cancer patients, these therapeutic processes have drawbacks of systemic toxicity and drug resistance .…”

Section: Discussionmentioning

confidence: 99%

“…Then, the membranes were incubated with horseradish peroxidase‐conjugated secondary antibodies at room temperature for 2 hours after washing. The signal was visualized using an enhanced chemiluminescence kit (Millipore, Billerica, Massachusetts) and quantified using an ImageQuant LAS 4000 Mini (GE Healthcare, Little Chalfont, Buckinghamshire, UK) …”

Section: Methodsmentioning

confidence: 99%

Licochalcone A induces apoptotic cell death via JNK/p38 activation in human nasopharyngeal carcinoma cells

Chuang

Tang

et al. 2019

Environmental Toxicology

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Licochalcone A is widely studied in different fields and possesses antiasthmatic, antibacterial, anti‐inflammatory, antioxidative, and anticancer properties. Its antimalignancy activity on renal, liver, lung, and oral cancer has been explored. However, limited studies have been conducted on the inhibitory effects of licochalcone A in human nasopharyngeal carcinoma cells. We determined cell viability using MTT assay. Cell cycle distribution and apoptotic cell death were measured via flow cytometry. Caspase activation and mitogen‐activated protein kinase‐related proteins in nasopharyngeal cancer cells in response to licochalcone A were identified by Western blot analysis. Results indicated that licochalcone A reduces cell viability and induces apoptosis, as evidenced by the upregulation of caspase‐8 and caspase‐9, caspase‐3 activation, and cleaved‐poly ADP‐ribose polymerase expression. Treatment with licochalcone A significantly increases ERK1/2, p38, and JNK1/2 activation. Co‐administration of a JNK inhibitor (JNK‐IN‐8) or p38 inhibitor (SB203580) abolishes the activation of caspase‐9, caspase‐8, and caspase‐3 protein expression during licochalcone A treatment. These findings indicate that licochalcone A exerts a cytostatic effect through apoptosis by targeting the JNK/p38 pathway in human nasopharyngeal carcinoma cells. Therefore, licochalcone A is a promising therapeutic agent for the treatment of human nasopharyngeal cancer cells.

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Hispolon suppresses migration and invasion of human nasopharyngeal carcinoma cells by inhibiting the urokinase‐plasminogen activator through modulation of the Akt signaling pathway

Cited by 32 publications

References 41 publications

Antimetastatic effects ofRheum palmatumL. extract on oral cancer cells

Antimetastatic effects ofRheum palmatumL. extract on oral cancer cells

Pinostilbene Hydrate Suppresses Human Oral Cancer Cell Metastasis by Downregulation of Matrix Metalloproteinase-2 Through the Mitogen-Activated Protein Kinase Signaling Pathway

Licochalcone A induces apoptotic cell death via JNK/p38 activation in human nasopharyngeal carcinoma cells

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