Hispolon suppresses migration and invasion of human nasopharyngeal carcinoma cells by inhibiting the urokinase‐plasminogen activator through modulation of the Akt signaling pathway

Abstract

Hispolon has been reported to possess antioxidant, antiinflammatory, and antitumor activities. However, the effect of hispolon on the metastasis of nasopharyngeal carcinoma (NPC) remains unclear. In this study, we investigated how the antimetastatic activity and relevant signaling pathways of hispolon affected three NPC cell lines. The results revealed that hispolon significantly reduced the migration and invasion of three NPC cells in a dose-dependent manner from 0 to 50 µM. Hispolon also significantly inhibited the activity and expression of urokinase-plasminogen activator (uPA) as well as the phosphorylation of Akt. Moreover, blocking the Akt pathway also enhanced the antimetastatic ability of hispolon in the NPC cells. In conclusion, hispolon inhibited uPA expression and NPC cell metastasis by downregulating Akt signal pathways; therefore, hispolon exerts beneficial effects in chemoprevention. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 645-655, 2017.

Hispolon induces apoptosis in oral squamous cell carcinoma cells through JNK/HO‐1 pathway activation

“…Huang et al have shown the apoptotic effect of hispolon by modulating ERK pathway on hepatocellular carcinoma cells 23 . Previously, our study also reported that hispolon suppressed metastasis on cervical cancer cells 24 and nasopharyngeal carcinoma cells 25 . Although the anticancer effect of hispolon has been found in various of tumour types, the mechanism of hispolon still lack on OSCC.…”

Hispolon induces apoptosis in oral squamous cell carcinoma cells through JNK/HO‐1 pathway activation

“…Huang et al have shown the apoptotic effect of hispolon by modulating ERK pathway on hepatocellular carcinoma cells 23 . Previously, our study also reported that hispolon suppressed metastasis on cervical cancer cells 24 and nasopharyngeal carcinoma cells 25 . Although the anticancer effect of hispolon has been found in various of tumour types, the mechanism of hispolon still lack on OSCC.…”

Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma

“…MMP-2, MMP-9 and epithelial-mesenchymal transition (EMT) markers were reported to be correlated with NPC metastasis [ 19 , 20 , 21 , 22 ]. We further confirmed expressions of these proteases by performing a Western blotting analysis.…”

Licochalcone A induces apoptotic cell death via JNK/p38 activation in human nasopharyngeal carcinoma cells

“…Herbal compositions from natural compounds have been utilized in preclinical or clinical trials with chemopreventive or therapeutic drugs for cancer management . Although chemotherapy and targeted therapy are effective methods for cancer patients, these therapeutic processes have drawbacks of systemic toxicity and drug resistance .…”

“…Herbal compositions from natural compounds have been utilized in preclinical or clinical trials with chemopreventive or therapeutic drugs for cancer management. 20,[23][24][25][26][27] Although chemotherapy and targeted therapy are effective methods for cancer patients, these therapeutic processes have drawbacks of systemic toxicity and drug resistance. 28,29 To overcome these problems, studies on combined therapy have focused on identifying phytotherapeutic agents with known action mechanisms that can augment the therapeutic index of chemotherapeutic agents, such as Lentinan combined with oxaliplatin in hepatocellular carcinoma, 30 baicalein combined with docetaxel in thyroid cancer cells, 31 curcumin combined with platinum in ovarian cancer cells, 32 and Astragalus polysaccharides combined with cisplatin in nasopharyngeal carcinoma cells.…”

Hispolon induces apoptosis in oral squamous cell carcinoma cells through JNK/HO‐1 pathway activation

“…Huang et al have shown the apoptotic effect of hispolon by modulating ERK pathway on hepatocellular carcinoma cells 23 . Previously, our study also reported that hispolon suppressed metastasis on cervical cancer cells 24 and nasopharyngeal carcinoma cells 25 . Although the anticancer effect of hispolon has been found in various of tumour types, the mechanism of hispolon still lack on OSCC.…”

Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma

“…MMP-2, MMP-9 and epithelial-mesenchymal transition (EMT) markers were reported to be correlated with NPC metastasis [ 19 , 20 , 21 , 22 ]. We further confirmed expressions of these proteases by performing a Western blotting analysis.…”

Licochalcone A induces apoptotic cell death via JNK/p38 activation in human nasopharyngeal carcinoma cells

“…Herbal compositions from natural compounds have been utilized in preclinical or clinical trials with chemopreventive or therapeutic drugs for cancer management . Although chemotherapy and targeted therapy are effective methods for cancer patients, these therapeutic processes have drawbacks of systemic toxicity and drug resistance .…”

“…Herbal compositions from natural compounds have been utilized in preclinical or clinical trials with chemopreventive or therapeutic drugs for cancer management. 20,[23][24][25][26][27] Although chemotherapy and targeted therapy are effective methods for cancer patients, these therapeutic processes have drawbacks of systemic toxicity and drug resistance. 28,29 To overcome these problems, studies on combined therapy have focused on identifying phytotherapeutic agents with known action mechanisms that can augment the therapeutic index of chemotherapeutic agents, such as Lentinan combined with oxaliplatin in hepatocellular carcinoma, 30 baicalein combined with docetaxel in thyroid cancer cells, 31 curcumin combined with platinum in ovarian cancer cells, 32 and Astragalus polysaccharides combined with cisplatin in nasopharyngeal carcinoma cells.…”

Hispolon induces apoptosis in oral squamous cell carcinoma cells through JNK/HO‐1 pathway activation

“…Huang et al have shown the apoptotic effect of hispolon by modulating ERK pathway on hepatocellular carcinoma cells 23 . Previously, our study also reported that hispolon suppressed metastasis on cervical cancer cells 24 and nasopharyngeal carcinoma cells 25 . Although the anticancer effect of hispolon has been found in various of tumour types, the mechanism of hispolon still lack on OSCC.…”

Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma

“…MMP-2, MMP-9 and epithelial-mesenchymal transition (EMT) markers were reported to be correlated with NPC metastasis [ 19 , 20 , 21 , 22 ]. We further confirmed expressions of these proteases by performing a Western blotting analysis.…”

Licochalcone A induces apoptotic cell death via JNK/p38 activation in human nasopharyngeal carcinoma cells

“…Herbal compositions from natural compounds have been utilized in preclinical or clinical trials with chemopreventive or therapeutic drugs for cancer management . Although chemotherapy and targeted therapy are effective methods for cancer patients, these therapeutic processes have drawbacks of systemic toxicity and drug resistance .…”

“…Herbal compositions from natural compounds have been utilized in preclinical or clinical trials with chemopreventive or therapeutic drugs for cancer management. 20,[23][24][25][26][27] Although chemotherapy and targeted therapy are effective methods for cancer patients, these therapeutic processes have drawbacks of systemic toxicity and drug resistance. 28,29 To overcome these problems, studies on combined therapy have focused on identifying phytotherapeutic agents with known action mechanisms that can augment the therapeutic index of chemotherapeutic agents, such as Lentinan combined with oxaliplatin in hepatocellular carcinoma, 30 baicalein combined with docetaxel in thyroid cancer cells, 31 curcumin combined with platinum in ovarian cancer cells, 32 and Astragalus polysaccharides combined with cisplatin in nasopharyngeal carcinoma cells.…”