Structure and evolution of Paramecium hemoglobin genes

A novel two‐over‐two α‐helical sandwich fold is characteristic of the truncated hemoglobin family

“…The P.caudatum globin gene has a single very short intron (29 bp) at position F3.0 (intron inserted between residue F2 and F3 codons), whereas C.eugametos displays introns at positions B6.0 (181 bp), E20.0 (306 bp) and F10.0 (755 bp) (Couture et al ., 1994; Yamauchi et al ., 1995). In contrast, the C.lacteus Hb gene has two introns, at positions B12.2 (intron inserted after the second base of the codon for residue B12) and G7.0; both intron positions are strongly conserved in the (non‐)vertebrate globin genes (Hankeln et al ., 1997; Dewilde et al ., 1998).…”

A novel two‐over‐two α‐helical sandwich fold is characteristic of the truncated hemoglobin family

“…The P.caudatum globin gene has a single very short intron (29 bp) at position F3.0 (intron inserted between residue F2 and F3 codons), whereas C.eugametos displays introns at positions B6.0 (181 bp), E20.0 (306 bp) and F10.0 (755 bp) (Couture et al ., 1994; Yamauchi et al ., 1995). In contrast, the C.lacteus Hb gene has two introns, at positions B12.2 (intron inserted after the second base of the codon for residue B12) and G7.0; both intron positions are strongly conserved in the (non‐)vertebrate globin genes (Hankeln et al ., 1997; Dewilde et al ., 1998).…”

Oxygen binding and NO scavenging properties of truncated hemoglobin, HbN, of Mycobacterium smegmatis

“…Physiological function(s) of M. smegmatis HbN is not very obvious at present. M. smegmatis HbN binds oxygen with reasonably high affinity, thus, there is a possibility that it may also participate in other metabolic reactions involving oxygen as has been envisaged for the protozoan and cyanobacterial HbN [27–29].…”

The Vacuolar‐ATPase of Paramecium multimicronucleatum: Gene Structure of the B Subunit and the Dynamics of the V‐ATPase‐rich Osmoregulatory Membranes

“…About 70 introns have been identified in these genes. Not only are they all very short, but they also are rich in A + T (Dupuis 1992; Russell et al 1994; Yamauchi et al 1992, 1995). The introns of the B subunit reported here are no exception.…”

“…9). Partial genomic libraries previously digested with Eco RI were ligated into the same restriction site of λXgtll (Yamauchi et al 1992, 1995) and screened by plaque hybridization (Huynh et al 1985). Recombinant DNA adsorbed on the nylon filters was hybridized overnight at 42 °C with 32 P‐labeled probe A or B (1 × 10 8 cpm/μg) in a hybridization buffer containing 10 × Denhardt's solution, 5X SSC, 50% formamide, 50 mM HEPES at pH 7.0, and 100 μg/ ml salmon sperm DNA (Sambrook et al 1989).…”

A novel two‐over‐two α‐helical sandwich fold is characteristic of the truncated hemoglobin family

“…The P.caudatum globin gene has a single very short intron (29 bp) at position F3.0 (intron inserted between residue F2 and F3 codons), whereas C.eugametos displays introns at positions B6.0 (181 bp), E20.0 (306 bp) and F10.0 (755 bp) (Couture et al ., 1994; Yamauchi et al ., 1995). In contrast, the C.lacteus Hb gene has two introns, at positions B12.2 (intron inserted after the second base of the codon for residue B12) and G7.0; both intron positions are strongly conserved in the (non‐)vertebrate globin genes (Hankeln et al ., 1997; Dewilde et al ., 1998).…”

Oxygen binding and NO scavenging properties of truncated hemoglobin, HbN, of Mycobacterium smegmatis

“…Physiological function(s) of M. smegmatis HbN is not very obvious at present. M. smegmatis HbN binds oxygen with reasonably high affinity, thus, there is a possibility that it may also participate in other metabolic reactions involving oxygen as has been envisaged for the protozoan and cyanobacterial HbN [27–29].…”

The Vacuolar‐ATPase of Paramecium multimicronucleatum: Gene Structure of the B Subunit and the Dynamics of the V‐ATPase‐rich Osmoregulatory Membranes

“…About 70 introns have been identified in these genes. Not only are they all very short, but they also are rich in A + T (Dupuis 1992; Russell et al 1994; Yamauchi et al 1992, 1995). The introns of the B subunit reported here are no exception.…”

“…9). Partial genomic libraries previously digested with Eco RI were ligated into the same restriction site of λXgtll (Yamauchi et al 1992, 1995) and screened by plaque hybridization (Huynh et al 1985). Recombinant DNA adsorbed on the nylon filters was hybridized overnight at 42 °C with 32 P‐labeled probe A or B (1 × 10 8 cpm/μg) in a hybridization buffer containing 10 × Denhardt's solution, 5X SSC, 50% formamide, 50 mM HEPES at pH 7.0, and 100 μg/ ml salmon sperm DNA (Sambrook et al 1989).…”

A novel two‐over‐two α‐helical sandwich fold is characteristic of the truncated hemoglobin family

“…The P.caudatum globin gene has a single very short intron (29 bp) at position F3.0 (intron inserted between residue F2 and F3 codons), whereas C.eugametos displays introns at positions B6.0 (181 bp), E20.0 (306 bp) and F10.0 (755 bp) (Couture et al ., 1994; Yamauchi et al ., 1995). In contrast, the C.lacteus Hb gene has two introns, at positions B12.2 (intron inserted after the second base of the codon for residue B12) and G7.0; both intron positions are strongly conserved in the (non‐)vertebrate globin genes (Hankeln et al ., 1997; Dewilde et al ., 1998).…”

Oxygen binding and NO scavenging properties of truncated hemoglobin, HbN, of Mycobacterium smegmatis

“…Physiological function(s) of M. smegmatis HbN is not very obvious at present. M. smegmatis HbN binds oxygen with reasonably high affinity, thus, there is a possibility that it may also participate in other metabolic reactions involving oxygen as has been envisaged for the protozoan and cyanobacterial HbN [27–29].…”

The Vacuolar‐ATPase of Paramecium multimicronucleatum: Gene Structure of the B Subunit and the Dynamics of the V‐ATPase‐rich Osmoregulatory Membranes

“…About 70 introns have been identified in these genes. Not only are they all very short, but they also are rich in A + T (Dupuis 1992; Russell et al 1994; Yamauchi et al 1992, 1995). The introns of the B subunit reported here are no exception.…”

“…9). Partial genomic libraries previously digested with Eco RI were ligated into the same restriction site of λXgtll (Yamauchi et al 1992, 1995) and screened by plaque hybridization (Huynh et al 1985). Recombinant DNA adsorbed on the nylon filters was hybridized overnight at 42 °C with 32 P‐labeled probe A or B (1 × 10 8 cpm/μg) in a hybridization buffer containing 10 × Denhardt's solution, 5X SSC, 50% formamide, 50 mM HEPES at pH 7.0, and 100 μg/ ml salmon sperm DNA (Sambrook et al 1989).…”