The growth of Micrococcus varians by utilizing sugar cane blackstrap molasses as substrate

Abstract

Comparative studies on the growth of Micrococcus varians were carried out in BHI culture medium (control) as well as in a culture medium with 2% diluted sugar cane blackstrap molasses, enriched with 0.1% yeast extract. The experiment was conducted with three samples of the experimental and control media in a 5 liter fermentor with working volume of 3.5 liters, continuous agitation (150 rpm), 35 ± 0.1 O C temperature, 0.7 L air. l -1 medium. min -1 , initial pH 7.0 ± 0.2, 24 hour fermentation period, and approximate inoculum of 6.0 log 10 CFU/ml. Samples were collected at 2-hour intervals. Micrococcus varians grew in the two culture media studied, which confirms the experimental medium viability for the growth of this species. The final average concentration of biomass was higher in the control medium than in the experimental medium: 0.99 g.l -1 and 0.78 g.l -1 , respectively. The final number of viable cells at the end of fermentation was 20.65 log 10 CFU/ml for the control medium (BHI), while in the experimental medium the number of viable cells was 19.43 log 10 CFU/ml. The consumption of total sugars was higher for the biomass in the control medium (79.78%), while only 50.53% was consumed for the experimental medium.

Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal

“…The mean sugar consumption values corresponding to treatments T3, T5 and T7 were 55.23, 43.97 and 42.96%, respectively. The range of sugar consumption percentage values is in agreement with the results obtained by Miranda et al (1999), in a study that found that the consumption of sugar reached 50.53% during fermentative growth of Micrococcus varians for 24 hours in a medium based on sugarcane molasses.…”

“…The least favorable condition was T9, with a biomass of 2.85 g L -1 , which was constituted by only 6% (m v -1 ) SCM, without protein supplementation (Table 1). These results are higher than those found by Miranda et al (1999), with M. varians production in a culture medium composed of SCM.…”

“…It can be observed that the total sugar content was 48.12%, with the highest percentage accounted for by non-reducing sugars, such as sucrose. Some studies on the use of SCM as a culture medium for the growth of microorganisms found similar values for the contents of total sugars, reducing sugars and non-reducing sugars, which had values of 48.50, 14.72 and 33.78%, respectively (Miranda et al, 1999). Feltrin, Sant'Anna, Porto, and Torres (2000) analyzed the composition of SCM and found higher values for the ash content than those obtained in this study.…”

“…Regarding viable cell count, T8 showed the best experimental outcome with 11.92 log CFU mL -1 followed by T4 (10.76 log CFU mL -1 ), T3 (10.43 log CFU mL -1 ), and T5 (10.11 log CFU mL -1 ) ( Table 1). Miranda et al (1999) found an approximate value of viable cells of M. varians after 12 hours of cultivation, obtaining 14 log CFU mL -1 of M. varians using a medium based on SCM, reaching the value of 19.43 log CFU mL -1 after 24 hours.…”

Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal

“…The mean sugar consumption values corresponding to treatments T3, T5 and T7 were 55.23, 43.97 and 42.96%, respectively. The range of sugar consumption percentage values is in agreement with the results obtained by Miranda et al (1999), in a study that found that the consumption of sugar reached 50.53% during fermentative growth of Micrococcus varians for 24 hours in a medium based on sugarcane molasses.…”

“…The least favorable condition was T9, with a biomass of 2.85 g L -1 , which was constituted by only 6% (m v -1 ) SCM, without protein supplementation (Table 1). These results are higher than those found by Miranda et al (1999), with M. varians production in a culture medium composed of SCM.…”

“…It can be observed that the total sugar content was 48.12%, with the highest percentage accounted for by non-reducing sugars, such as sucrose. Some studies on the use of SCM as a culture medium for the growth of microorganisms found similar values for the contents of total sugars, reducing sugars and non-reducing sugars, which had values of 48.50, 14.72 and 33.78%, respectively (Miranda et al, 1999). Feltrin, Sant'Anna, Porto, and Torres (2000) analyzed the composition of SCM and found higher values for the ash content than those obtained in this study.…”

“…Regarding viable cell count, T8 showed the best experimental outcome with 11.92 log CFU mL -1 followed by T4 (10.76 log CFU mL -1 ), T3 (10.43 log CFU mL -1 ), and T5 (10.11 log CFU mL -1 ) ( Table 1). Miranda et al (1999) found an approximate value of viable cells of M. varians after 12 hours of cultivation, obtaining 14 log CFU mL -1 of M. varians using a medium based on SCM, reaching the value of 19.43 log CFU mL -1 after 24 hours.…”

Identifikasi dan Uji Kemampuan Hidrolisis pada Bakteri Amilolitik dan Proteolitik yang Diisolasi dari Wadi, Makanan Khas Kalimantan Tengah

“…Micrococcus varians memiliki ciri-ciri berbentuk kokus, berdiameter 0,5-2,0 μm, gram positif. Micrococcus varians memiliki karakteristik dapat memberikan warna dan rasa pada produk fermentasi (Miranda et al, 1999).…”

Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal

“…The mean sugar consumption values corresponding to treatments T3, T5 and T7 were 55.23, 43.97 and 42.96%, respectively. The range of sugar consumption percentage values is in agreement with the results obtained by Miranda et al (1999), in a study that found that the consumption of sugar reached 50.53% during fermentative growth of Micrococcus varians for 24 hours in a medium based on sugarcane molasses.…”

“…The least favorable condition was T9, with a biomass of 2.85 g L -1 , which was constituted by only 6% (m v -1 ) SCM, without protein supplementation (Table 1). These results are higher than those found by Miranda et al (1999), with M. varians production in a culture medium composed of SCM.…”

“…It can be observed that the total sugar content was 48.12%, with the highest percentage accounted for by non-reducing sugars, such as sucrose. Some studies on the use of SCM as a culture medium for the growth of microorganisms found similar values for the contents of total sugars, reducing sugars and non-reducing sugars, which had values of 48.50, 14.72 and 33.78%, respectively (Miranda et al, 1999). Feltrin, Sant'Anna, Porto, and Torres (2000) analyzed the composition of SCM and found higher values for the ash content than those obtained in this study.…”

“…Regarding viable cell count, T8 showed the best experimental outcome with 11.92 log CFU mL -1 followed by T4 (10.76 log CFU mL -1 ), T3 (10.43 log CFU mL -1 ), and T5 (10.11 log CFU mL -1 ) ( Table 1). Miranda et al (1999) found an approximate value of viable cells of M. varians after 12 hours of cultivation, obtaining 14 log CFU mL -1 of M. varians using a medium based on SCM, reaching the value of 19.43 log CFU mL -1 after 24 hours.…”

Identifikasi dan Uji Kemampuan Hidrolisis pada Bakteri Amilolitik dan Proteolitik yang Diisolasi dari Wadi, Makanan Khas Kalimantan Tengah

“…Micrococcus varians memiliki ciri-ciri berbentuk kokus, berdiameter 0,5-2,0 μm, gram positif. Micrococcus varians memiliki karakteristik dapat memberikan warna dan rasa pada produk fermentasi (Miranda et al, 1999).…”

Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal

“…The mean sugar consumption values corresponding to treatments T3, T5 and T7 were 55.23, 43.97 and 42.96%, respectively. The range of sugar consumption percentage values is in agreement with the results obtained by Miranda et al (1999), in a study that found that the consumption of sugar reached 50.53% during fermentative growth of Micrococcus varians for 24 hours in a medium based on sugarcane molasses.…”

“…The least favorable condition was T9, with a biomass of 2.85 g L -1 , which was constituted by only 6% (m v -1 ) SCM, without protein supplementation (Table 1). These results are higher than those found by Miranda et al (1999), with M. varians production in a culture medium composed of SCM.…”

“…It can be observed that the total sugar content was 48.12%, with the highest percentage accounted for by non-reducing sugars, such as sucrose. Some studies on the use of SCM as a culture medium for the growth of microorganisms found similar values for the contents of total sugars, reducing sugars and non-reducing sugars, which had values of 48.50, 14.72 and 33.78%, respectively (Miranda et al, 1999). Feltrin, Sant'Anna, Porto, and Torres (2000) analyzed the composition of SCM and found higher values for the ash content than those obtained in this study.…”

“…Regarding viable cell count, T8 showed the best experimental outcome with 11.92 log CFU mL -1 followed by T4 (10.76 log CFU mL -1 ), T3 (10.43 log CFU mL -1 ), and T5 (10.11 log CFU mL -1 ) ( Table 1). Miranda et al (1999) found an approximate value of viable cells of M. varians after 12 hours of cultivation, obtaining 14 log CFU mL -1 of M. varians using a medium based on SCM, reaching the value of 19.43 log CFU mL -1 after 24 hours.…”

Identifikasi dan Uji Kemampuan Hidrolisis pada Bakteri Amilolitik dan Proteolitik yang Diisolasi dari Wadi, Makanan Khas Kalimantan Tengah

“…Micrococcus varians memiliki ciri-ciri berbentuk kokus, berdiameter 0,5-2,0 μm, gram positif. Micrococcus varians memiliki karakteristik dapat memberikan warna dan rasa pada produk fermentasi (Miranda et al, 1999).…”