The growth of Micrococcus varians by utilizing sugar cane blackstrap molasses as substrate

Abstract

Comparative studies on the growth of Micrococcus varians were carried out in BHI culture medium (control) as well as in a culture medium with 2% diluted sugar cane blackstrap molasses, enriched with 0.1% yeast extract. The experiment was conducted with three samples of the experimental and control media in a 5 liter fermentor with working volume of 3.5 liters, continuous agitation (150 rpm), 35 ± 0.1 O C temperature, 0.7 L air. l -1 medium. min -1 , initial pH 7.0 ± 0.2, 24 hour fermentation period, and approximate inoculum of 6.0 log 10 CFU/ml. Samples were collected at 2-hour intervals. Micrococcus varians grew in the two culture media studied, which confirms the experimental medium viability for the growth of this species. The final average concentration of biomass was higher in the control medium than in the experimental medium: 0.99 g.l -1 and 0.78 g.l -1 , respectively. The final number of viable cells at the end of fermentation was 20.65 log 10 CFU/ml for the control medium (BHI), while in the experimental medium the number of viable cells was 19.43 log 10 CFU/ml. The consumption of total sugars was higher for the biomass in the control medium (79.78%), while only 50.53% was consumed for the experimental medium.

Identifikasi dan Uji Kemampuan Hidrolisis pada Bakteri Amilolitik dan Proteolitik yang Diisolasi dari Wadi, Makanan Khas Kalimantan Tengah

“…Micrococcus varians memiliki ciri-ciri berbentuk kokus, berdiameter 0,5-2,0 μm, gram positif. Micrococcus varians memiliki karakteristik dapat memberikan warna dan rasa pada produk fermentasi (Miranda et al, 1999).…”

“…Micrococcus varians merupakan bakteri yang biasa ditemukan dalam starter untuk fermentasi daging. M. varian berperan untuk membentuk warna dan cita rasa yang khas (Miranda et al, 1999). Hal tersebut juga didukung oleh pendapat Khairina & Khotimah (2006) dalam penelitiannya mengenai wadi dari ikan betok (Anabas testudineus Bloch) menyatakan bahwa rasa yang khas pada wadi sebagai salah satu produk fermentasi disebabkan oleh adanya degradasi protein dan lemak pada substrat.…”

Production of extra cellular enzymes by microbial strains in molasses and additives supplemented fermentation media

“…The production of sugarcane in Brazil in 2012/2013 harvest reached 589 million tons, yielding from 23 to 35 tons of molasses (CONAB, 2013). Its composition and abundance makes this residue a potential substrate for the development of biotechnological processes, including the production of enzymes and other products (Miranda et al, 1999).…”

Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal

“…To enable the large-scale industrial production of starter cultures, low-cost culture media that ensure good bacterial growth are essential (Makowski et al, 2017). Obtained commercially, the medium that is currently used extensively for multiplication of Staphylococcus is Brain Heart Infusion broth (BHI) (Miranda, Sant'anna, & Porto, 1999).…”