The runt domain identifies a new family of heterometric transcriptional regulators

Transactivation of the Moloney murine leukemia virus and T-cell receptor beta-chain enhancers by cbf and ets requires intact binding sites for both proteins

“…As in the case of the P19 cells and endogenous ets proteins, we propose that S3 cells contain saturating levels of an endogenous CBF homolog. This proposal is consistent with the observations that the Drosophila Runt/Brother heterodimer binds the same consensus core site as the mammalian CBF complex (19,36).…”

Transactivation of the Moloney murine leukemia virus and T-cell receptor beta-chain enhancers by cbf and ets requires intact binding sites for both proteins

“…As in the case of the P19 cells and endogenous ets proteins, we propose that S3 cells contain saturating levels of an endogenous CBF homolog. This proposal is consistent with the observations that the Drosophila Runt/Brother heterodimer binds the same consensus core site as the mammalian CBF complex (19,36).…”

The Capacity of Polyomavirus Enhancer Binding Protein 2αB (AML1/Cbfa2) To Stimulate Polyomavirus DNA Replication Is Related to Its Affinity for the Nuclear Matrix

“…All the mutants showed DNA binding activity except 70-451 (data not shown). 70-451 lacks the 20-aa region of the Runt domain that is essential for DNA binding activity (28). In addition, we confirmed that PEBP2␣B1 and its deletion derivatives were capable of forming heterodimers with the ␤ subunit because anti-␤ subunit antibody caused a supershift of each of the shifted bands in EMSA (data not shown).…”

Species-specific deployment of Runx2 isoforms and differential regulation of target genes during avian jaw development and evolution

“…Conservation of the RHD is even higher within amniotes and within each major clade. This is not surprising given that the RHD is an ancient 128 AA DNA binding domain that arose early in metazoan evolution and defines the RUNX protein family (Kagoshima et al, 1993;Rennert et al, 2003;Levanon and Groner, 2004;Coffman, 2009;Robertson et al, 2009;Chuang et al, 2013;Ito et al, 2015;Mevel et al, 2019). Interestingly in reptiles (including birds), we find that the RHD is modified by one AA, which goes from phenylalanine (F) to leucine (L).…”

Transactivation of the Moloney murine leukemia virus and T-cell receptor beta-chain enhancers by cbf and ets requires intact binding sites for both proteins

“…As in the case of the P19 cells and endogenous ets proteins, we propose that S3 cells contain saturating levels of an endogenous CBF homolog. This proposal is consistent with the observations that the Drosophila Runt/Brother heterodimer binds the same consensus core site as the mammalian CBF complex (19,36).…”

The Capacity of Polyomavirus Enhancer Binding Protein 2αB (AML1/Cbfa2) To Stimulate Polyomavirus DNA Replication Is Related to Its Affinity for the Nuclear Matrix

“…All the mutants showed DNA binding activity except 70-451 (data not shown). 70-451 lacks the 20-aa region of the Runt domain that is essential for DNA binding activity (28). In addition, we confirmed that PEBP2␣B1 and its deletion derivatives were capable of forming heterodimers with the ␤ subunit because anti-␤ subunit antibody caused a supershift of each of the shifted bands in EMSA (data not shown).…”

Species-specific deployment of Runx2 isoforms and differential regulation of target genes during avian jaw development and evolution

“…Conservation of the RHD is even higher within amniotes and within each major clade. This is not surprising given that the RHD is an ancient 128 AA DNA binding domain that arose early in metazoan evolution and defines the RUNX protein family (Kagoshima et al, 1993;Rennert et al, 2003;Levanon and Groner, 2004;Coffman, 2009;Robertson et al, 2009;Chuang et al, 2013;Ito et al, 2015;Mevel et al, 2019). Interestingly in reptiles (including birds), we find that the RHD is modified by one AA, which goes from phenylalanine (F) to leucine (L).…”

Transactivation of the Moloney murine leukemia virus and T-cell receptor beta-chain enhancers by cbf and ets requires intact binding sites for both proteins

“…As in the case of the P19 cells and endogenous ets proteins, we propose that S3 cells contain saturating levels of an endogenous CBF homolog. This proposal is consistent with the observations that the Drosophila Runt/Brother heterodimer binds the same consensus core site as the mammalian CBF complex (19,36).…”

The Capacity of Polyomavirus Enhancer Binding Protein 2αB (AML1/Cbfa2) To Stimulate Polyomavirus DNA Replication Is Related to Its Affinity for the Nuclear Matrix

“…All the mutants showed DNA binding activity except 70-451 (data not shown). 70-451 lacks the 20-aa region of the Runt domain that is essential for DNA binding activity (28). In addition, we confirmed that PEBP2␣B1 and its deletion derivatives were capable of forming heterodimers with the ␤ subunit because anti-␤ subunit antibody caused a supershift of each of the shifted bands in EMSA (data not shown).…”

Species-specific deployment of Runx2 isoforms and differential regulation of target genes during avian jaw development and evolution

“…Conservation of the RHD is even higher within amniotes and within each major clade. This is not surprising given that the RHD is an ancient 128 AA DNA binding domain that arose early in metazoan evolution and defines the RUNX protein family (Kagoshima et al, 1993;Rennert et al, 2003;Levanon and Groner, 2004;Coffman, 2009;Robertson et al, 2009;Chuang et al, 2013;Ito et al, 2015;Mevel et al, 2019). Interestingly in reptiles (including birds), we find that the RHD is modified by one AA, which goes from phenylalanine (F) to leucine (L).…”