Silica Particles with Human Protein Corona Shows Sensitization Potential in the Human Cell Line Activation Test

Eto, Shun‐ichi; Koshida, Aoi; Tsujino, Hirofumi; Nagano, Kazuya; Higashisaka, Kazuma; Tsutsumi, Yasuo

doi:10.1248/bpbreports.5.1_1

Cited by 1 publication

(2 citation statements)

References 18 publications

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“…Interestingly, inflammatory response by silica NP through this receptor showed size specific. In a recent report, protein corona formation on the surface of silica particles showed sensitization potential to THP-1 cells ( Eto et al, 2022 ). Hence, it is a forthcoming challenge to increase the variety of test materials and analyze the relationship between APCs activation and other properties, including affinity to SR-B1 and protein absorption properties.…”

Section: Resultsmentioning

confidence: 96%

“…To date, the immunotoxicity of NMs has been independently investigated using several kinds of cells, such as murine dendritic cells ( Winter et al, 2011 ), murine macrophage-like RAW 264.7 cells ( Alsaleh et al, 2019 ), rat NR8383 alveolar macrophages ( Wiemann et al, 2016 ), murine macrophage cell line J774A.1 cells and human lung adenocarcinoma cell line A549 cells ( Breznan et al, 2017 ), human monocytic cell line THP-1 cells ( Nishijima et al, 2017a ; Cui et al, 2020 ; Murugadoss et al, 2020 ; Eto et al, 2022 ), and differentiated THP-1 cells ( Brzicova et al, 2019 ) with a variety of evaluation indices, such as CD80, CD86, CD54, major histocompatibility complex (MHC)-II, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. Therefore, it is essential to develop a standardized in vitro method to evaluate the immunotoxicity of NMs.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the immunotoxicity potential of nanomaterials using THP-1 cells

Nishida,

Sawada,

Arai

et al. 2024

Front. Toxicol.

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With the expansion of nanomaterials (NMs) usage, concerns about their toxicity are increasing, and the wide variety of NMs makes it difficult to assess their toxicity. Therefore, the development of a high-throughput, accurate, and certified method to evaluate the immunotoxicity of NMs is required. In this study, we assessed the immunotoxicity potential of various NMs, such as nanoparticles of silver, silica, and titanium dioxide, using the human Cell Line Activation Test (h-CLAT) at the cellular level. After exposure to silver nanoparticle dispersions, the expression levels of CD86 and CD54 increased, suggesting the activation of antigen-presenting cells (APCs) by silver nanoparticles. Quantification of silver ions eluted from silver nanoparticles and the activation of APCs by silver ions suggested that it was due to the release of silver ions. Silica nanoparticles also increased the expression of CD86 and/or CD54, and their activation ability correlated with the synthesis methods and hydrodynamic diameters. The ability of titanium dioxide to activate APCs differed depending on the crystal type and hydrodynamic diameter. These results suggest a potential method to evaluate the immunotoxicity potential of various NMs based on their ability to activate APCs using human monocytic THP-1 cells. This method will be valuable in assessing the immunotoxicity potential and elucidating the immunotoxic mechanisms of NMs.

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Section: Resultsmentioning

confidence: 96%