RNA-Based Next-Generation Sequencing in Non-Small-Cell Lung Cancer in a Routine Setting: an Experience from an Italian Referral Center

Gene Fusion Detection in NSCLC Routine Clinical Practice: Targeted-NGS or FISH?

“…We analyzed a total of 210 NSCLC samples by FISH, and we succeeded in characterizing 200 of these samples (95%) by targeted RNA NGS and 198 samples (94%) by RT-PCR. The results of our analyses, together with already published data [ 17 , 18 , 19 , 20 ], showed an excellent concordance rate (91%) of the targeted RNA NGS results with the so-far considered gold standard FISH technique, underlining the essential role of the NGS testing approach in the molecular pathology diagnostics laboratory.…”

Gene Fusion Detection in NSCLC Routine Clinical Practice: Targeted-NGS or FISH?

“…We analyzed a total of 210 NSCLC samples by FISH, and we succeeded in characterizing 200 of these samples (95%) by targeted RNA NGS and 198 samples (94%) by RT-PCR. The results of our analyses, together with already published data [ 17 , 18 , 19 , 20 ], showed an excellent concordance rate (91%) of the targeted RNA NGS results with the so-far considered gold standard FISH technique, underlining the essential role of the NGS testing approach in the molecular pathology diagnostics laboratory.…”

FGFR2 testing in cholangiocarcinoma: translating molecular studies into clinical practice

“…Overall, NGS may represent a more impacting diagnostic method than FISH in the detection of FGFR2 fusions and allows the detection of multiple genetic alterations in tissue samples with scarce material ( Fig. 2 ) 59 , 80 .…”

A New Approach of Detecting ALK Fusion Oncogenes by RNA Sequencing Exon Coverage Analysis

“…With the exception of the first method, all of these enrichment techniques allow for the sequencing of all fusions involving target genes, even with unknown fusion partners. NGS panels have demonstrated high sensitivity and specificity for fusion detection [ 43 , 44 , 45 ]. The European Society for Medical Oncology (ESMO) recommends their use in routine practice for analyzing fusions in NSCLC samples [ 46 ].…”

Gene Fusion Detection in NSCLC Routine Clinical Practice: Targeted-NGS or FISH?

“…We analyzed a total of 210 NSCLC samples by FISH, and we succeeded in characterizing 200 of these samples (95%) by targeted RNA NGS and 198 samples (94%) by RT-PCR. The results of our analyses, together with already published data [ 17 , 18 , 19 , 20 ], showed an excellent concordance rate (91%) of the targeted RNA NGS results with the so-far considered gold standard FISH technique, underlining the essential role of the NGS testing approach in the molecular pathology diagnostics laboratory.…”

FGFR2 testing in cholangiocarcinoma: translating molecular studies into clinical practice

“…Overall, NGS may represent a more impacting diagnostic method than FISH in the detection of FGFR2 fusions and allows the detection of multiple genetic alterations in tissue samples with scarce material ( Fig. 2 ) 59 , 80 .…”

A New Approach of Detecting ALK Fusion Oncogenes by RNA Sequencing Exon Coverage Analysis

“…With the exception of the first method, all of these enrichment techniques allow for the sequencing of all fusions involving target genes, even with unknown fusion partners. NGS panels have demonstrated high sensitivity and specificity for fusion detection [ 43 , 44 , 45 ]. The European Society for Medical Oncology (ESMO) recommends their use in routine practice for analyzing fusions in NSCLC samples [ 46 ].…”

Gene Fusion Detection in NSCLC Routine Clinical Practice: Targeted-NGS or FISH?

“…We analyzed a total of 210 NSCLC samples by FISH, and we succeeded in characterizing 200 of these samples (95%) by targeted RNA NGS and 198 samples (94%) by RT-PCR. The results of our analyses, together with already published data [ 17 , 18 , 19 , 20 ], showed an excellent concordance rate (91%) of the targeted RNA NGS results with the so-far considered gold standard FISH technique, underlining the essential role of the NGS testing approach in the molecular pathology diagnostics laboratory.…”

FGFR2 testing in cholangiocarcinoma: translating molecular studies into clinical practice

“…Overall, NGS may represent a more impacting diagnostic method than FISH in the detection of FGFR2 fusions and allows the detection of multiple genetic alterations in tissue samples with scarce material ( Fig. 2 ) 59 , 80 .…”

A New Approach of Detecting ALK Fusion Oncogenes by RNA Sequencing Exon Coverage Analysis

“…With the exception of the first method, all of these enrichment techniques allow for the sequencing of all fusions involving target genes, even with unknown fusion partners. NGS panels have demonstrated high sensitivity and specificity for fusion detection [ 43 , 44 , 45 ]. The European Society for Medical Oncology (ESMO) recommends their use in routine practice for analyzing fusions in NSCLC samples [ 46 ].…”