Quantitation and molecular species determination of diacylglycerols, phosphatidylcholines, ceramides, and sphingomyelins with gas chromatography

ESI-MS quantitation of increased sphingomyelin in Niemann-Pick disease type B HDL

“…The nearly constant SM-to-PC ratios were also observed when using the HPLC/MS system on the same samples (data not shown). The values obtained here were within the biological range reported in various studies using mass spectrometry (33,34).…”

“…Because the amount of PC was more than 9-fold greater than that of SM in plasma HDL analytes, PC ions, especially those of the dominant species, were easily identified. These were PCs with 34 and PC species described here and their relative abundances were consistent with what had been reported for other kinds of biological samples, such as blood, red blood cells, and liver homogenates (18,(32)(33)(34). We did not acquire product ion spectra for acyl anions of a few minor PCs because their intensities were too low.…”

ESI-MS quantitation of increased sphingomyelin in Niemann-Pick disease type B HDL

“…The nearly constant SM-to-PC ratios were also observed when using the HPLC/MS system on the same samples (data not shown). The values obtained here were within the biological range reported in various studies using mass spectrometry (33,34).…”

“…Because the amount of PC was more than 9-fold greater than that of SM in plasma HDL analytes, PC ions, especially those of the dominant species, were easily identified. These were PCs with 34 and PC species described here and their relative abundances were consistent with what had been reported for other kinds of biological samples, such as blood, red blood cells, and liver homogenates (18,(32)(33)(34). We did not acquire product ion spectra for acyl anions of a few minor PCs because their intensities were too low.…”

Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis

“…HPTLC can only quantify the relative intensity of sphingomyelin and is not possible to avoid the interfering compounds on TLC chromatogram [2]. For the GC analysis of sphingomyelin, the sensitivity and quality of analyses vary due to the high column temperature [3]. Plasma sphingomyelin from different animal species has been quantified by 31 P NMR analysis [4].…”

Quantification of ceramide species in biological samples by liquid chromatography electrospray ionization tandem mass spectrometry

“…Several HPLC [18], HPLC-MS [24, 29, 36] and gas GC-MS [19] approaches have been described for determining concentrations in biological samples. Although HPLC and GC-MS instruments are easily available and widely used for measuring ceramide concentrations, all of these techniques require derivatization prior to analysis.…”

ESI-MS quantitation of increased sphingomyelin in Niemann-Pick disease type B HDL

“…The nearly constant SM-to-PC ratios were also observed when using the HPLC/MS system on the same samples (data not shown). The values obtained here were within the biological range reported in various studies using mass spectrometry (33,34).…”

“…Because the amount of PC was more than 9-fold greater than that of SM in plasma HDL analytes, PC ions, especially those of the dominant species, were easily identified. These were PCs with 34 and PC species described here and their relative abundances were consistent with what had been reported for other kinds of biological samples, such as blood, red blood cells, and liver homogenates (18,(32)(33)(34). We did not acquire product ion spectra for acyl anions of a few minor PCs because their intensities were too low.…”

Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis

“…HPTLC can only quantify the relative intensity of sphingomyelin and is not possible to avoid the interfering compounds on TLC chromatogram [2]. For the GC analysis of sphingomyelin, the sensitivity and quality of analyses vary due to the high column temperature [3]. Plasma sphingomyelin from different animal species has been quantified by 31 P NMR analysis [4].…”

Quantification of ceramide species in biological samples by liquid chromatography electrospray ionization tandem mass spectrometry

“…Several HPLC [18], HPLC-MS [24, 29, 36] and gas GC-MS [19] approaches have been described for determining concentrations in biological samples. Although HPLC and GC-MS instruments are easily available and widely used for measuring ceramide concentrations, all of these techniques require derivatization prior to analysis.…”

ESI-MS quantitation of increased sphingomyelin in Niemann-Pick disease type B HDL

“…The nearly constant SM-to-PC ratios were also observed when using the HPLC/MS system on the same samples (data not shown). The values obtained here were within the biological range reported in various studies using mass spectrometry (33,34).…”

“…Because the amount of PC was more than 9-fold greater than that of SM in plasma HDL analytes, PC ions, especially those of the dominant species, were easily identified. These were PCs with 34 and PC species described here and their relative abundances were consistent with what had been reported for other kinds of biological samples, such as blood, red blood cells, and liver homogenates (18,(32)(33)(34). We did not acquire product ion spectra for acyl anions of a few minor PCs because their intensities were too low.…”

Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis

“…HPTLC can only quantify the relative intensity of sphingomyelin and is not possible to avoid the interfering compounds on TLC chromatogram [2]. For the GC analysis of sphingomyelin, the sensitivity and quality of analyses vary due to the high column temperature [3]. Plasma sphingomyelin from different animal species has been quantified by 31 P NMR analysis [4].…”

Quantification of ceramide species in biological samples by liquid chromatography electrospray ionization tandem mass spectrometry

“…Several HPLC [18], HPLC-MS [24, 29, 36] and gas GC-MS [19] approaches have been described for determining concentrations in biological samples. Although HPLC and GC-MS instruments are easily available and widely used for measuring ceramide concentrations, all of these techniques require derivatization prior to analysis.…”