Quantitation and molecular species determination of diacylglycerols, phosphatidylcholines, ceramides, and sphingomyelins with gas chromatography

Tserng, Kou‐Yi; Griffin, Ronda

doi:10.1016/j.ab.2003.08.026

Cited by 68 publications

(53 citation statements)

References 41 publications

(39 reference statements)

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“…C16-ceramide content in the LV was measured by a capillary gas chromatographic procedure with a flame ionization detector using C17-ceramide as an internal standard, as previously described (51). Tissue TG content was measured in homogenate extracts using an enzymatic spectrophotometer method (Wako Chemicals, Richmond, VA).…”

Section: Methodsmentioning

confidence: 99%

High-fat diet postinfarction enhances mitochondrial function and does not exacerbate left ventricular dysfunction

Rennison¹,

McElfresh²,

Okere³

et al. 2007

American Journal of Physiology-Heart and Circulatory Physiology

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November 17, 2006; doi:10.1152/ajpheart.01021.2006.-Lipid accumulation in nonadipose tissue due to enhanced circulating fatty acids may play a role in the pathophysiology of heart failure, obesity, and diabetes. Accumulation of myocardial lipids and related intermediates, e.g., ceramide, is associated with decreased contractile function, mitochondrial oxidative phosphorylation, and electron transport chain (ETC) complex activities. We tested the hypothesis that the progression of heart failure would be exacerbated by elevated myocardial lipids and an associated ceramide-induced inhibition of mitochondrial oxidative phosphorylation and ETC complex activities. Heart failure (HF) was induced by coronary artery ligation. Rats were then randomly assigned to either a normal (10% kcal from fat; HF, n ϭ 8) or high saturated fat diet (60% kcal from saturated fat; HF ϩ Sat, n ϭ 7). Sham-operated animals (sham; n ϭ 8) were fed a normal diet. Eight weeks postligation, left ventricular (LV) function was assessed by echocardiography and catheterization. Subsarcolemmal and interfibrillar mitochondria were isolated from the LV. Heart failure resulted in impaired LV contractile function [decreased percent fractional shortening and peak rate of LV pressure rise and fall (ϮdP/dt)] and remodeling (increased end-diastolic and end-systolic dimensions) in HF compared with sham. No further progression of LV dysfunction was evident in HF ϩ Sat. Mitochondrial state 3 respiration was increased in HF ϩ Sat compared with HF despite elevated myocardial ceramide. Activities of ETC complexes II and IV were elevated in HF ϩ Sat compared with HF and sham. High saturated fat feeding following coronary artery ligation was associated with increased oxidative phosphorylation and ETC complex activities and did not adversely affect LV contractile function or remodeling, despite elevations in myocardial ceramide. oxidative phosphorylation; electron transport chain; ceramide; lipotoxicity FATTY ACIDS (FA) are the dominant energy source for the adult mammalian heart and also are utilized for membrane biosynthesis, generation of lipid signaling molecules, posttranslational protein modification, and transcriptional regulation (43). Chronic exposure to FA can result in an imbalance between FA uptake and utilization that potentially can trigger cytotoxic mechanisms, leading to cell dysfunction or death, a phenomenon known as lipotoxicity. Extensive clinical and animal studies have shown that excess lipid accumulation in nonadipose tissue due to enhanced circulating FA may play an important role in pathophysiological conditions such as heart failure, obesity, insulin resistance, and diabetes (15,43,58).A loss of synchronization between FA availability and utilization in cardiomyocytes, despite otherwise normal or upregulated ␤-oxidation capacity, can lead to an increase in the accumulation of tissue ceramide (24). Ceramide, a lipid signaling molecule, has been implicated in the formation of reactive oxygen species and peroxidation of membrane lipids (11), a...

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Section: Methodsmentioning

confidence: 99%

High-fat diet postinfarction enhances mitochondrial function and does not exacerbate left ventricular dysfunction

Rennison¹,

McElfresh²,

Okere³

et al. 2007

American Journal of Physiology-Heart and Circulatory Physiology

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“…Therefore, most methods rely on hydrolysis of phospholipids by phospholipase C to diacylglycerols. This is followed by methyl transesterification, producing fatty acid methyl esters (FAMEs) that are subsequently analyzed by GC (Tserng and Griffin, 2003). The first application of GC for fatty acids was in the 1950s (Bondia-Pons et al, 2004).…”

Section: Gas Chromatographymentioning

confidence: 99%

A review of chromatographic methods for the assessment of phospholipids in biological samples

Peterson

Cummings

2005

Biomedical Chromatography

233

159

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Phospholipids are important constituents of all living cell membranes. Lipidomics is a rapidly growing field that provides insight as to how specific phospholipids play roles in normal physiological and disease states. There are many analytical methods available for the qualitative and quantitative determination of phospholipids. This review provides a summary of the methods that were historically used such as thin layer chromatography, gas chromatography and high-performance liquid chromatography. In addition, an introduction to applications of interfacing these traditional chromatographic techniques with mass spectrometry is provided.

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“…Radiation-induced increase in ceramide localized to mitochondria was required for activation of CED-3 caspase and apoptosis. These studies underscore the physiological significance of the mitochondrial ceramide and SM pools (Andreyev et al, 2010;Ardail et al, 2001;Dai et al, 2004;Monette et al, 2010;Tserng and Griffin, 2003;Yabu et al, 2009). Although several enzyme activities involved in ceramide metabolism have been demonstrated in mitochondria, the nature of enzymes generating ceramide in this organelle is still a matter of debate (Laviad et al, 2008).…”

Section: Ceramide Generation In Mitochondriamentioning

confidence: 99%

“…Mitochondria arise as important intracellular compartment for ceramide metabolism, and they have been shown to contain a variety of sphingolipids, including SM and ceramide (Ardail et al, 2001;Tserng and Griffin, 2003). Mounting evidence suggests a local action of ceramide on mitochondria in intact cells.…”

Section: Ceramide Generation In Mitochondriamentioning

confidence: 99%

Mitochondrial Ceramide in Stroke

Gudz¹,

Novgorodov²

2012

Advances in the Preclinical Study of Ischemic Stroke

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Quantitation and molecular species determination of diacylglycerols, phosphatidylcholines, ceramides, and sphingomyelins with gas chromatography

Cited by 68 publications

References 41 publications

High-fat diet postinfarction enhances mitochondrial function and does not exacerbate left ventricular dysfunction

High-fat diet postinfarction enhances mitochondrial function and does not exacerbate left ventricular dysfunction

A review of chromatographic methods for the assessment of phospholipids in biological samples

Mitochondrial Ceramide in Stroke

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