Protective effect of coconut water concentrate and its active component shikimic acid against hydroperoxide mediated oxidative stress through suppression of NF-κB and activation of Nrf2 pathway

Curcumin inhibits superoxide anion-induced pain-like behavior and leukocyte recruitment by increasing Nrf2 expression and reducing NF-κB activation

“…In response to oxidative and electrophilic stress, Keap1 is inactivated, and Nrf2 translocates to nucleus and binds at a promoter sequence known as the antioxidant-responsive element (ARE) that is found in many cyto-protective genes. In general, target genes of Nrf2 are involved in glutathione synthesis and elimination of ROS, providing a protective function against oxidative stress [60,61]. Evidence demonstrates that curcumin increases Nrf2 activity [27,28], which lines up well with a 1.5-fold increase in Nrf2 mRNA expression found in our study.…”

Gold-conjugated green tea nanoparticles for enhanced anti-tumor activities and hepatoprotection — synthesis, characterization and in vitro evaluation

“…Single cell suspensions were made in RPMI 1640 by chopping a portion of the liver with a blunt forceps and passing the cell population through a nylon mesh with 70 μm pore size. Red blood cells were lysed by incubating the cells in 0.83% ammonium chloride solution for 10 minutes at 4°C [38,39]. Cell viability was determined using Trypan blue exclusion test and 10 6 cells/ml isolated cells were plated in 35 mm plates in RPMI 1640 medium in presence of 100 U/ml of Streptomycin and 50 U/ml Gentamycin for 24 hours.…”

“…After 24 hours the medium was aspirated and fresh medium was added then the cells were cultured under the different experimental conditions for 24 h at 37°C in a CO 2 incubator [38].…”

Nickel(II) complexes having different configurations controlled by N,N,O-donor Schiff-base ligands in presence of isothiocyanate as co-ligand: Synthesis, structures, comparative biological activity and DFT study

“…The cell population was passed through a nylon mesh with 70 lM pore size. Cell viability was determined using Trypan blue exclusion test and then the cells were cultured in the same way the EAC cells were cultured [35,36].…”