Pectobacterium carotovorum subsp. brasiliense and Pectobacterium carotovorum subsp. carotovorum as causal agents of potato soft rot in Algeria

Naas, Hiba; Sebaihia, Mohammed; Orfei, Benedetta; Rezzonico, Fabio; Buonaurio, Roberto; Moretti, Chiaraluce

doi:10.1007/s10658-018-1438-3

Cited by 25 publications

(22 citation statements)

References 53 publications

(64 reference statements)

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“…The international distribution of the genus Pectobacterium increases concerns about the economic losses caused by this bacterium to the potato growers. A study in the neighboring country Algeria, carried out between 2014 and 2015, revealed the presence of pectinolytic bacteria causing soft rot in potatoes that belonged to P. brasiliense and P. carotovorum , as judged by MLSA [ 63 ]. Ozturk et al reported the presence of P. atrosepticum , P. brasiliense , P. carotovorum and P. parmentieri species in Turkey [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

Diversity of Pectobacteriaceae Species in Potato Growing Regions in Northern Morocco

Oulghazi

Moumni

Khayi³

et al. 2020

Microorganisms

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Dickeya and Pectobacterium pathogens are causative agents of several diseases that affect many crops worldwide. This work investigated the species diversity of these pathogens in Morocco, where Dickeya pathogens have only been isolated from potato fields recently. To this end, samplings were conducted in three major potato growing areas over a three-year period (2015–2017). Pathogens were characterized by sequence determination of both the gapA gene marker and genomes using Illumina and Oxford Nanopore technologies. We isolated 119 pathogens belonging to P. versatile (19%), P. carotovorum (3%), P. polaris (5%), P. brasiliense (56%) and D. dianthicola (17%). Their taxonomic assignation was confirmed by draft genome analyses of 10 representative strains of the collected species. D. dianthicola were isolated from a unique area where a wide species diversity of pectinolytic pathogens was observed. In tuber rotting assays, D. dianthicola isolates were more aggressive than Pectobacterium isolates. The complete genome sequence of D. dianthicola LAR.16.03.LID was obtained and compared with other D. dianthicola genomes from public databases. Overall, this study highlighted the ecological context from which some Dickeya and Pectobacterium species emerged in Morocco, and reported the first complete genome of a D. dianthicola strain isolated in Morocco that will be suitable for further epidemiological studies.

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Section: Discussionmentioning

confidence: 99%

Diversity of Pectobacteriaceae Species in Potato Growing Regions in Northern Morocco

Oulghazi

Moumni

Khayi³

et al. 2020

Microorganisms

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“…Pectobacterium brasiliense (Pbr) is a worldwide-distributed bacterial plant pathogen causing soft rot of a wide range of economically important crops. Pbr has been reported in Eurasia: from Western European countries to Russia and China [ 4 , 5 , 6 ], in Africa: South Africa, Morocco, Algeria and Kenya [ 7 , 8 , 9 , 10 ]. Pbr has been constantly reported since 2004 causing significant losses particularly of potato ( Solanum tuberosum L.) [ 11 , 12 , 13 , 14 ] and posing a threat to worldwide potato gross production value which is estimated to be $63914 million in 2018 [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Pectobacterium brasiliense: Genomics, Host Range and Disease Management

et al. 2021

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Pectobacterium brasiliense (Pbr) is considered as one of the most virulent species among the Pectobacteriaceae. This species has a broad host range within horticulture crops and is well distributed elsewhere. It has been found to be pathogenic not only in the field causing blackleg and soft rot of potato, but it is also transmitted via storage causing soft rot of other vegetables. Genomic analysis and other cost-effective molecular detection methods such as a quantitative polymerase chain reaction (qPCR) are essential to investigate the ecology and pathogenesis of the Pbr. The lack of fast, field deployable point-of-care testing (POCT) methods, specific control strategies and current limited genomic knowledge make management of this species difficult. Thus far, no comprehensive review exists about Pbr, however there is an intense need to research the biology, detection, pathogenicity and management of Pbr, not only because of its fast distribution across Europe and other countries but also due to its increased survival to various climatic conditions. This review outlines the information available in peer-reviewed literature regarding host range, detection methods, genomics, geographical distribution, nomenclature and taxonomical evolution along with some of the possible management and control strategies. In summary, the conclusions and a further directions highlight the management of this species.

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“…In 2016, severe wet rot (tip over) in banana plants caused by Pcc was recorded in greenhouses in Turkey (Basim et al, 2019). Pcc is also a major pathogen of tuber soft rot in different countries and results in severe economic losses (Muturi et al, 2018;Naas et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Potential of a Quorum Quenching Bacteria Isolate Ochrobactrum intermedium D-2 Against Soft Rot Pathogen Pectobacterium carotovorum subsp. carotovorum

Fan

Tian

et al. 2020

Front. Microbiol.

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Quorum quenching (QQ) is a promising strategy for preventing and controlling quorum sensing (QS)-mediated bacterial infections. It interferes with QS by the inhibition of signal synthesis, the detection of enzyme-catalyzed degradation, and the modification of signals. N-Acyl homoserine lactones (AHLs) represent a family of widely conserved QS signals involved in the regulation of virulence factor production in many Gram-negative bacterial pathogens. In this study, AHL-degrading bacterial strains were isolated, and the most efficient one was evaluated for its potential against QS-mediated pathogens. Results showed that an AHL-degrading bacteria Ochrobactrum intermedium D-2 effectively attenuated maceration produced by the pathogen Pectobacterium carotovorum subsp. carotovorum (Pcc) on radish and potato slices. Strain D-2 exhibited a superior AHL degradation activity and efficiently degraded various AHLs, including N-hexanoyl-L-homoserine lactone (C6HSL), N-(3-oxohexanoyl)-L-homoserine lactone (3OC6HSL), N-(3-oxooctanoyl)-L-homoserine lactone (3OC8HSL), and N-(3-oxododecanoyl)-L-homoserine lactone (3OC12HSL). Analysis of the degradation products of AHL by gas chromatography-mass spectrometry led to the identification of N-cyclohexyl-propanamide and propanamide as the main intermediate products, suggesting that AHL was degraded by hydrolysis. Annotation and analysis of the whole genome sequence of strain D-2 revealed the presence of an AHL-lactonase, termed AidF. Moreover, the application of strain D-2 was able to substantially reduce the disease severity caused by Pcc on host plants. These results reveal the biochemical basis of a highly efficient AHL-degrading bacterial isolate and present the potential to attenuate Pcc virulence through QQ.

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Pectobacterium carotovorum subsp. brasiliense and Pectobacterium carotovorum subsp. carotovorum as causal agents of potato soft rot in Algeria

Cited by 25 publications

References 53 publications

Diversity of Pectobacteriaceae Species in Potato Growing Regions in Northern Morocco

Diversity of Pectobacteriaceae Species in Potato Growing Regions in Northern Morocco

Pectobacterium brasiliense: Genomics, Host Range and Disease Management

Potential of a Quorum Quenching Bacteria Isolate Ochrobactrum intermedium D-2 Against Soft Rot Pathogen Pectobacterium carotovorum subsp. carotovorum

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