Low Doses of Hyperbaric Oxygenation Effectively Decrease the Size of Necrotic Zone in Rats with Experimental Myocardial Infarction

Abstract

We studied the effect of a single session of hyperbaric oxygenation on the size of risk, ischemic, and necrotic zones in rat myocardium after irreversible occlusion of the coronary artery and excessive oxygen pressure of 0.02 and 0.1 MPa. Myocardium infarction was reproduced by ligation of the left coronary artery. The size of the risk, ischemic, and necrotic zones was planimetrically evaluated. Hyperbaric oxygenation (60-min session) was performed 3 h after artery occlusion at excessive oxygen pressure of 0.02 and 0.1 MPa. In rats not exposed to hyperbaric oxygenation, the risk zone median was 31.7% of the left ventricle weight, while after the session it did not exceed 25%. In spontaneous course of myocardium infarction, the ischemia to necrosis zone ratio was 1.7:1, while under conditions of hyperbaric oxygenation at oxygen pressure of 0.1 and 0.02 MPa, the these values were 0.6:1 and 2:1, respectively. Excessive oxygen pressure of 0.02 mPa is better than traditionally used 0.1 MPa, because it promotes redistribution of the ischemic and necrotic areas in the risk zone: the area of necrotic zone decreased at the expense of the ischemic zone. Hyperbaric oxygenation produces a positive effect on the myocardium under conditions of total occlusion of the coronary artery.

Effect of intermittent hyperoxia on stem cell mobilization and cytokine expression

“…The field of therapeutic IH is dominated by the use of HBO, and HBO therapy is approved by the FDA to treat 15 indications 24. Additionally, although not FDA approved for these indications, studies appear to suggest that HBO therapy may ameliorate other conditions including myocardial infarction,25 hip fractures,2627282930 stroke,331323334353637 peri-surgical healing,3839404142 and traumatic brain injury 33543444546…”

Effect of intermittent hyperoxia on stem cell mobilization and cytokine expression

“…The field of therapeutic IH is dominated by the use of HBO, and HBO therapy is approved by the FDA to treat 15 indications 24. Additionally, although not FDA approved for these indications, studies appear to suggest that HBO therapy may ameliorate other conditions including myocardial infarction,25 hip fractures,2627282930 stroke,331323334353637 peri-surgical healing,3839404142 and traumatic brain injury 33543444546…”

Hyperbaric oxygen‐induced long non‐coding RNA MALAT1 exosomes suppress MicroRNA‐92a expression in a rat model of acute myocardial infarction

“…4 HBO was shown to induce cardioprotection through anti-apoptotic effect and decreased infarct size in a rat model of MI. 5,6 Several small clinical trials also demonstrated the beneficial effect of HBO in patients with acute coronary syndrome by reducing the infarct size, and the risk of major cardiovascular events and death. 7 Exosomes are 40-90 nm-sized extracellular vesicles of endosomal origin that play a crucial role in intercellular processes.…”

Hemoglobin concentration maintained despite reduced erythrocyte count and hematocrit during exercise under hyperbaric oxygen conditions in healthy adult males: A randomized crossover trial

“…Exercise also has a considerable significant effect on blood components, reducing the expression of pro-inflammatory cytokines such as tumor necrosis factor-α and IL-6, and reportedly contributes to the suppression of inflammatory responses (8). Research on HBO environments has mostly been conducted using animal models such as rats, sheep, and rabbits (9, 10), and few studies involving humans have been conducted. The combination of an HBO environment and high-intensity interval training moderately induce ROS; promotes NO production, which results in improved vascular endothelial function (11).…”

Effect of intermittent hyperoxia on stem cell mobilization and cytokine expression

“…The field of therapeutic IH is dominated by the use of HBO, and HBO therapy is approved by the FDA to treat 15 indications 24. Additionally, although not FDA approved for these indications, studies appear to suggest that HBO therapy may ameliorate other conditions including myocardial infarction,25 hip fractures,2627282930 stroke,331323334353637 peri-surgical healing,3839404142 and traumatic brain injury 33543444546…”

Hyperbaric oxygen‐induced long non‐coding RNA MALAT1 exosomes suppress MicroRNA‐92a expression in a rat model of acute myocardial infarction

“…4 HBO was shown to induce cardioprotection through anti-apoptotic effect and decreased infarct size in a rat model of MI. 5,6 Several small clinical trials also demonstrated the beneficial effect of HBO in patients with acute coronary syndrome by reducing the infarct size, and the risk of major cardiovascular events and death. 7 Exosomes are 40-90 nm-sized extracellular vesicles of endosomal origin that play a crucial role in intercellular processes.…”

Hemoglobin concentration maintained despite reduced erythrocyte count and hematocrit during exercise under hyperbaric oxygen conditions in healthy adult males: A randomized crossover trial

“…Exercise also has a considerable significant effect on blood components, reducing the expression of pro-inflammatory cytokines such as tumor necrosis factor-α and IL-6, and reportedly contributes to the suppression of inflammatory responses (8). Research on HBO environments has mostly been conducted using animal models such as rats, sheep, and rabbits (9, 10), and few studies involving humans have been conducted. The combination of an HBO environment and high-intensity interval training moderately induce ROS; promotes NO production, which results in improved vascular endothelial function (11).…”

Effect of intermittent hyperoxia on stem cell mobilization and cytokine expression

“…The field of therapeutic IH is dominated by the use of HBO, and HBO therapy is approved by the FDA to treat 15 indications 24. Additionally, although not FDA approved for these indications, studies appear to suggest that HBO therapy may ameliorate other conditions including myocardial infarction,25 hip fractures,2627282930 stroke,331323334353637 peri-surgical healing,3839404142 and traumatic brain injury 33543444546…”

Hyperbaric oxygen‐induced long non‐coding RNA MALAT1 exosomes suppress MicroRNA‐92a expression in a rat model of acute myocardial infarction

“…4 HBO was shown to induce cardioprotection through anti-apoptotic effect and decreased infarct size in a rat model of MI. 5,6 Several small clinical trials also demonstrated the beneficial effect of HBO in patients with acute coronary syndrome by reducing the infarct size, and the risk of major cardiovascular events and death. 7 Exosomes are 40-90 nm-sized extracellular vesicles of endosomal origin that play a crucial role in intercellular processes.…”

Hemoglobin concentration maintained despite reduced erythrocyte count and hematocrit during exercise under hyperbaric oxygen conditions in healthy adult males: A randomized crossover trial

“…Exercise also has a considerable significant effect on blood components, reducing the expression of pro-inflammatory cytokines such as tumor necrosis factor-α and IL-6, and reportedly contributes to the suppression of inflammatory responses (8). Research on HBO environments has mostly been conducted using animal models such as rats, sheep, and rabbits (9, 10), and few studies involving humans have been conducted. The combination of an HBO environment and high-intensity interval training moderately induce ROS; promotes NO production, which results in improved vascular endothelial function (11).…”