In vitro feeding assays for hard ticks

Kröber, Thomas; Guérin, Patrick M.

doi:10.1016/j.pt.2007.07.010

Cited by 108 publications

(99 citation statements)

References 19 publications

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“…Silicone membranes have been used successfully to feed adults of Dermacentor nuttalli Olenev, 1928 and Hyalomma excavatum Koch, 1844 (formerly H . anatolicum excavatum ) (Habedank, Hiepe & Montag, 1994) and all life stages of Amblyomma hebraeum Koch, 1844 (Kuhnert, Diehl & Guerin, 1995) and Ixodes ricinus (Kroeber & Guerin, 2007). Even though the technique must be adapted and modified for use with each tick species according to the length of the mouthparts (Kroeber & Guerin, 2007), examples of successful feeding of other species such as Rhipicephalus sanguineus Latreille, 1806, Hyalomma dromedarii Koch, 1844 and H. anatolicum Koch, 1844 (formerly H. anatolicum anatolicum ) (Tajeri & Razmi, 2011; Fourie et al ., 2013) suggest this is a useful approach for cement research (Fig.…”

Section: Historical Perspective On Cement Researchmentioning

confidence: 99%

Tick attachment cement – reviewing the mysteries of a biological skin plug system

et al. 2017

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The majority of ticks in the family Ixodidae secrete a substance anchoring their mouthparts to the host skin. This substance is termed cement. It has adhesive properties and seals the lesion during feeding. The particular chemical composition and the curing process of the cement are unclear. This review summarizes the literature, starting with a historical overview, briefly introducing the different hypotheses on the origin of the adhesive and how the tick salivary glands have been identified as its source. Details on the sequence of cement deposition, the curing process and detachment are provided. Other possible functions of the cement, such as protection from the host immune system and antimicrobial properties, are presented. Histochemical and ultrastructural data of the intracellular granules in the salivary gland cells, as well as the secreted cement, suggest that proteins constitute the main material, with biochemical data revealing glycine to be the dominant amino acid. Applied methods and their restrictions are discussed. Tick cement is compared with adhesives of other animals such as barnacles, mussels and sea urchins. Finally, we address the potential of tick cement for the field of biomaterial research and in particular for medical applications in future.

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Section: Historical Perspective On Cement Researchmentioning

confidence: 99%

Tick attachment cement – reviewing the mysteries of a biological skin plug system

et al. 2017

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“…Although in vitro feeding assays have been shown to be useful in studying different tick molecules and tick-pathogen interaction [70], its application in RNAi study in ticks has been limited. A study on the Lyme disease vector I. scapularis employed capillary feeding of dsRNA to nymphs to suppress anticomplement gene isac [71].…”

Section: Feedingmentioning

confidence: 99%

RNA Interference – A Powerful Functional Analysis Tool for Studying Tick Biology and its Control

Galay¹,

Umemiya‐Shirafuji²,

MasamiMochizuki³

et al. 2016

RNA Interference

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Ticks (Acari: Ixodida) are blood-sucking arthropods globally recognized as vectors of numerous diseases. They are primarily responsible for the transmission of various pathogens, including viruses, rickettsiae, and blood parasites of animals. Ticks are second to mosquitoes in terms of disease transmission to humans. The continuous emergence of tick-borne diseases and acaricide resistance of ticks necessitates the development of new and more effective control agents and strategies; therefore, understanding of different aspects of tick biology and their interaction with pathogens is very crucial in developing effective control strategies. RNA interference (RNAi) has been widely used in the area of tick research as a versatile reverse genetic tool to elucidate the functions of various tick proteins. During the past decade, numerous studies on ticks utilized RNAi to evaluate potentially key tick proteins involved in blood feeding, reproduction, evasion of host immune response, interaction with pathogens, and pathogen transmission that may be targeted for tick and pathogen control. This chapter reviewed the application of RNAi in tick research over the past decade, focusing on the impact of this technique in the advancement of knowledge on tick and pathogen biology.

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“…It is easy to understand that the risk increases with the tick feeding and engorging. Nevertheless, there are few studies Science Publications AJID based in animal (De Silva and Fikrig, 1995;Lindsay et al, 1997;Katavolos et al, 1998;Zivkovic et al, 2007;Horka et al, 2009) or in vitro (Krober and Guerin, 2007;Hojgaard et al, 2008) models that evaluate the time of feeding for the transmission of tick-borne agents.…”

Section: Fig 1 Life Cycle Of the Hard Tick Ixodes Ricinusmentioning

confidence: 99%

Prevention and Prophylaxis of Tick Bites and Tick-Borne Related Diseases

García‐Álvarez¹,

Palomar²,

Oteo³

2013

American Journal of Infectious Diseases

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Ticks are obligate haematophagous arthropods present all over the world able to produce human diseases. Several factors have increased the abundance, circulation and distribution of the pathogens transmitted by ticks, contributing to the change in the vector-borne diseases epidemiology in the last years. This review collects the most important measures for the prevention and prophylaxis of tick-borne diseases. The preexposition measures to avoid tick-borne diseases are based on the prevention of tick bites by avoiding tickinfested areas, using of protective clothing, repellents and controlling tick populations by physical, mechanical, biological and chemical methods. It is also reviewed other measures as the utility of educational programs and the use of human vaccines. On the other hand, we also review some key aspects referred to the measures to carry out after tick bites as how to remove a tick correctly and the utility of making an antibiotic prophylaxis.

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In vitro feeding assays for hard ticks

Cited by 108 publications

References 19 publications

Tick attachment cement – reviewing the mysteries of a biological skin plug system

Tick attachment cement – reviewing the mysteries of a biological skin plug system

RNA Interference – A Powerful Functional Analysis Tool for Studying Tick Biology and its Control

Prevention and Prophylaxis of Tick Bites and Tick-Borne Related Diseases

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