Hexadecylphosphocholine alters nonvesicular cholesterol traffic from the plasma membrane to the endoplasmic reticulum and inhibits the synthesis of sphingomyelin in HepG2 cells

Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models

“…For instance, the decrease of SM content after treatment with IC75 EPC3 was ~24% for MCF-7 cells and ~16% for MDA-MB-231 cells as compared with the control samples. This finding is in line with the observations of Marco and coworkers [47] who showed that treatment of human hepatoma HepG2 cell line with Miltefosine led to inhibition of SM metabolism. Disturbances in SM synthesis could cause the accumulation of ceramide and sphingosine, which regulate cellular functions such as proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis.…”

Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models

“…For instance, the decrease of SM content after treatment with IC75 EPC3 was ~24% for MCF-7 cells and ~16% for MDA-MB-231 cells as compared with the control samples. This finding is in line with the observations of Marco and coworkers [47] who showed that treatment of human hepatoma HepG2 cell line with Miltefosine led to inhibition of SM metabolism. Disturbances in SM synthesis could cause the accumulation of ceramide and sphingosine, which regulate cellular functions such as proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis.…”

Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models

“…It is therefore tempting to assume that the changes in membrane order (observed by using Di-4-ANEPPDQH rather than Laurdan) might be connected to alterations in membrane compositions brought about by EPC3. This hypothesis is in agreement with the observation that certain APLs might influence lipid metabolism and lipid-mediated signaling cascades (1,2,43). Also EPC3 can reduce the synthesis of key raft-associated phospholipids, such as PC and SM ((44) and Tzoneva R., unpublished data).…”

Disruption of cellular cholesterol transport and homeostasis as a novel mechanism of action of membrane‐targeted alkylphospholipid analogues

“…Previously, we demonstrated that 50 µM HePC blocked the esterification of plasma membrane cholesterol, confirming that the inhibition caused by HePC in the esterification of the intracellular cholesterol pool is produced by a disruption of its movement from the plasma membrane to the ER (Marco et al. , 2009).…”

Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models

“…For instance, the decrease of SM content after treatment with IC75 EPC3 was ~24% for MCF-7 cells and ~16% for MDA-MB-231 cells as compared with the control samples. This finding is in line with the observations of Marco and coworkers [47] who showed that treatment of human hepatoma HepG2 cell line with Miltefosine led to inhibition of SM metabolism. Disturbances in SM synthesis could cause the accumulation of ceramide and sphingosine, which regulate cellular functions such as proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis.…”

Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models

“…It is therefore tempting to assume that the changes in membrane order (observed by using Di-4-ANEPPDQH rather than Laurdan) might be connected to alterations in membrane compositions brought about by EPC3. This hypothesis is in agreement with the observation that certain APLs might influence lipid metabolism and lipid-mediated signaling cascades (1,2,43). Also EPC3 can reduce the synthesis of key raft-associated phospholipids, such as PC and SM ((44) and Tzoneva R., unpublished data).…”

Disruption of cellular cholesterol transport and homeostasis as a novel mechanism of action of membrane‐targeted alkylphospholipid analogues

“…Previously, we demonstrated that 50 µM HePC blocked the esterification of plasma membrane cholesterol, confirming that the inhibition caused by HePC in the esterification of the intracellular cholesterol pool is produced by a disruption of its movement from the plasma membrane to the ER (Marco et al. , 2009).…”

Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models

“…For instance, the decrease of SM content after treatment with IC75 EPC3 was ~24% for MCF-7 cells and ~16% for MDA-MB-231 cells as compared with the control samples. This finding is in line with the observations of Marco and coworkers [47] who showed that treatment of human hepatoma HepG2 cell line with Miltefosine led to inhibition of SM metabolism. Disturbances in SM synthesis could cause the accumulation of ceramide and sphingosine, which regulate cellular functions such as proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis.…”

Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models

“…It is therefore tempting to assume that the changes in membrane order (observed by using Di-4-ANEPPDQH rather than Laurdan) might be connected to alterations in membrane compositions brought about by EPC3. This hypothesis is in agreement with the observation that certain APLs might influence lipid metabolism and lipid-mediated signaling cascades (1,2,43). Also EPC3 can reduce the synthesis of key raft-associated phospholipids, such as PC and SM ((44) and Tzoneva R., unpublished data).…”

Disruption of cellular cholesterol transport and homeostasis as a novel mechanism of action of membrane‐targeted alkylphospholipid analogues

“…Previously, we demonstrated that 50 µM HePC blocked the esterification of plasma membrane cholesterol, confirming that the inhibition caused by HePC in the esterification of the intracellular cholesterol pool is produced by a disruption of its movement from the plasma membrane to the ER (Marco et al. , 2009).…”