Distribution and function of 3′,5′-Cyclic-AMP phosphodiesterases in the human ovary

Expression of genes and enzymes involved in ovarian steroidogenesis in relation to human follicular development

“…Data from both the microarray and the RNA results confirm that, but in pre-OI follicles, preceding the final maturation of follicles, the microarray data demonstrated gene expression above the background. Since our microarray data were validated by RT-PCR, which has been previously published ( 8 – 11 ), we hypothesize that this observation may be attributed to a minor contamination of TCs during the aspiration of preovulatory follicles, which had not yet been exposed to ovulation triggers, and the surrounding cells remained tightly packed.…”

Expression of genes and enzymes involved in ovarian steroidogenesis in relation to human follicular development

“…Data from both the microarray and the RNA results confirm that, but in pre-OI follicles, preceding the final maturation of follicles, the microarray data demonstrated gene expression above the background. Since our microarray data were validated by RT-PCR, which has been previously published ( 8 – 11 ), we hypothesize that this observation may be attributed to a minor contamination of TCs during the aspiration of preovulatory follicles, which had not yet been exposed to ovulation triggers, and the surrounding cells remained tightly packed.…”

Phosphodiesterases in the rat ovary: effect of cAMP in primordial follicles

“…We saw a relative low expression of Pde4d compared to the enzymatic activity of PDE4. Recently, similar results have been reported in human GCs (Petersen et al 2015). This could be due to a rapid turnover of the mRNA as seen with other transcripts involved in signalling (Schwanhausser et al 2011).…”

“…Low level of Pde8b was seen in contrast to that observed in bovine ovaries (Sasseville et al 2009) and in humans (Markholt et al 2012, Petersen et al 2015. Instead, Pde8a appeared to be the major subtype present, especially in the CL.…”

PDE4 inhibitor Roflumilast modulates inflammation and lipid accumulation in PCOS mice to improve ovarian function and reduce DHEA‐induced granulosa cell apoptosis in vitro

“…In PCOS patients, follicles represent growth arrest and degenerative changes, with progressive aggregation of follicular fluid as well as extension of the antrum (Chang & Cook-Andersen, 2013). Besides, a case of previous study revealed that PDE4 inhibition could increase the secretion of progesterone and ameliorate aging-related metabolic diseases (Park et al, 2012;Petersen et al, 2015). Roflumilast, which is selective PDE4 inhibitor, has been widely applied in the treatment of diseases.…”

Expression of genes and enzymes involved in ovarian steroidogenesis in relation to human follicular development

“…Data from both the microarray and the RNA results confirm that, but in pre-OI follicles, preceding the final maturation of follicles, the microarray data demonstrated gene expression above the background. Since our microarray data were validated by RT-PCR, which has been previously published ( 8 – 11 ), we hypothesize that this observation may be attributed to a minor contamination of TCs during the aspiration of preovulatory follicles, which had not yet been exposed to ovulation triggers, and the surrounding cells remained tightly packed.…”

Phosphodiesterases in the rat ovary: effect of cAMP in primordial follicles

“…We saw a relative low expression of Pde4d compared to the enzymatic activity of PDE4. Recently, similar results have been reported in human GCs (Petersen et al 2015). This could be due to a rapid turnover of the mRNA as seen with other transcripts involved in signalling (Schwanhausser et al 2011).…”

“…Low level of Pde8b was seen in contrast to that observed in bovine ovaries (Sasseville et al 2009) and in humans (Markholt et al 2012, Petersen et al 2015. Instead, Pde8a appeared to be the major subtype present, especially in the CL.…”

PDE4 inhibitor Roflumilast modulates inflammation and lipid accumulation in PCOS mice to improve ovarian function and reduce DHEA‐induced granulosa cell apoptosis in vitro

“…In PCOS patients, follicles represent growth arrest and degenerative changes, with progressive aggregation of follicular fluid as well as extension of the antrum (Chang & Cook-Andersen, 2013). Besides, a case of previous study revealed that PDE4 inhibition could increase the secretion of progesterone and ameliorate aging-related metabolic diseases (Park et al, 2012;Petersen et al, 2015). Roflumilast, which is selective PDE4 inhibitor, has been widely applied in the treatment of diseases.…”

Expression of genes and enzymes involved in ovarian steroidogenesis in relation to human follicular development

“…Data from both the microarray and the RNA results confirm that, but in pre-OI follicles, preceding the final maturation of follicles, the microarray data demonstrated gene expression above the background. Since our microarray data were validated by RT-PCR, which has been previously published ( 8 – 11 ), we hypothesize that this observation may be attributed to a minor contamination of TCs during the aspiration of preovulatory follicles, which had not yet been exposed to ovulation triggers, and the surrounding cells remained tightly packed.…”

Phosphodiesterases in the rat ovary: effect of cAMP in primordial follicles

“…We saw a relative low expression of Pde4d compared to the enzymatic activity of PDE4. Recently, similar results have been reported in human GCs (Petersen et al 2015). This could be due to a rapid turnover of the mRNA as seen with other transcripts involved in signalling (Schwanhausser et al 2011).…”

“…Low level of Pde8b was seen in contrast to that observed in bovine ovaries (Sasseville et al 2009) and in humans (Markholt et al 2012, Petersen et al 2015. Instead, Pde8a appeared to be the major subtype present, especially in the CL.…”

PDE4 inhibitor Roflumilast modulates inflammation and lipid accumulation in PCOS mice to improve ovarian function and reduce DHEA‐induced granulosa cell apoptosis in vitro

“…In PCOS patients, follicles represent growth arrest and degenerative changes, with progressive aggregation of follicular fluid as well as extension of the antrum (Chang & Cook-Andersen, 2013). Besides, a case of previous study revealed that PDE4 inhibition could increase the secretion of progesterone and ameliorate aging-related metabolic diseases (Park et al, 2012;Petersen et al, 2015). Roflumilast, which is selective PDE4 inhibitor, has been widely applied in the treatment of diseases.…”