Association between COX-2 rs 6681231 Genotype and Interleukin-6 in Periodontal Connective Tissue. A Pilot Study

Abstract

ObjectivesThe aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes.DesignA case-control study included 41 gingival biopsies obtained from Caucasian patients grouped according to clinical diagnosis of gingival health (n = 10), gingivitis (n = 15) or chronic periodontitis (n = 16). Immunohistochemistry analyses were performed to determine COX-2 expression in lamina propria, IL-6 expression in lamina propria and gingival epithelium and level of inflammatory cell infiltrate. Individual DNA was extracted and genotyped by real-time PCR for IL6 SNPs rs 2069827 and rs 2069825 and for COX-2 rs 6681231.ResultsThe percentage of cellular COX-2 expression was associated with the extent of periodontal disease (Arbes index p = 0.026) and inflammatory infiltrate (p<0.0001). No association was observed between IL6 haplotypes and cells positive to IL-6 or COX-2 in gingival tissues. The COX-2 rs 6681231 was associated with cells positive to IL-6 in the connective tissue (p = 0.032).ConclusionsCOX-2 expression in gingival tissues may be a marker of periodontal disease severity. COX-2 rs 6681231 may be associated with IL-6 local production in gingival tissues.

Colorectal Carcinogenesis: Role of Oxidative Stress and Antioxidants

“…Currently, it is clearly documented that any dysregulation or imbalance of the gut microbiota might lead to changes in colocytes as well as extraintestinal disease (5,6). This disruption causes an extensive and persisting inflammatory reaction, manifested by up-regulation of an array of pro-inflammatory mediators both locally and systemically, such as; cyclooxygenase 2 (COX-2), prostaglandin E 2 (PGE 2 ), tumor necrosis factor α (TNF-α), nuclear factor kappa B (NFkB), and transforming growth factor β (TGFβ) among others (8)(9)(10). Persistent inflammatory reaction, in its turn, leads to the development of IBD by activating the Gut Associated Lymphoid Tissue (GALT) (4).…”

COX2 and NOS3 gene polymorphisms in women with gestational diabetes

“…In recent study, it was shown that the CC COX2 rs6681231 genotype was not correlated with COX2 expression itself; however, in periodontal connective tissue, it was associated with the number of cells that are positive to IL-6. 44 The results of the present study suggest that that the CC genotype of the COX2 rs6681231 polymorphism is associated with an increased risk of GDM and the need for insulin therapy, whereas the TT genotype of the NOS3 rs1799983 polymorphism may be associated with the need for insulin therapy in women with GDM, although this hypothesis requires further investigation.…”

“…have indicated that COX2 gene polymorphism is a risk factor for incident stroke in African Americans. In recent study, it was shown that the CC COX2 rs6681231 genotype was not correlated with COX2 expression itself; however, in periodontal connective tissue, it was associated with the number of cells that are positive to IL‐6 …”

Impact of host gene polymorphisms on susceptibility to chronic hepatitis B virus infection

“…Some studies demonstrated that polymorphisms of this gene can affect the concentration of IL-6 in serum (Erciyas et al, 2010;Mesa et al, 2014). Polymorphisms in promoter region of IL-6 gene affect transcription and expression of IL-6 in individuals.…”