A Glyphosate-Based Herbicide Cross-Selects for Antibiotic Resistance Genes in Bacterioplankton Communities

Costa, Naíla Barbosa da; Hébert, Marie‐Pier; Fugère, Vincent; Terrat, Yves; Fussmann, Gregor F.; Gonzalez, Andrew; Shapiro, B. Jesse

doi:10.1128/msystems.01482-21

Cited by 16 publications

(7 citation statements)

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“…This phenomenon is most likely explainable by the existence of efficient efflux pumps that are able to export absorbed small molecules in an unchanged form. Those efflux transporters have been recognized as highly important in bacterial antibiotic and, interestingly, also in herbicide (glyphosate) resistance [ 46 , 47 ]. Horizontal gene transfer, for instance, via plasmids, contributes to a rapid distribution of this avoidance strategy within a bacterial community.…”

Section: Resultsmentioning

confidence: 99%

Bioactive Nitrosylated and Nitrated N-(2-hydroxyphenyl)acetamides and Derived Oligomers: An Alternative Pathway to 2-Amidophenol-Derived Phytotoxic Metabolites

et al. 2022

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Incubation of Aminobacter aminovorans, Paenibacillus polymyxa, and Arthrobacter MPI764 with the microbial 2-benzoxazolinone (BOA)-degradation-product 2-acetamido-phenol, produced from 2-aminophenol, led to the recently identified N-(2-hydroxy-5-nitrophenyl) acetamide, to the hitherto unknown N-(2-hydroxy-5-nitrosophenyl)acetamide, and to N-(2-hydroxy-3-nitrophenyl)acetamide. As an alternative to the formation of phenoxazinone derived from aminophenol, dimers- and trimers-transformation products have been found. Identification of the compounds was carried out by LC/HRMS and MS/MS and, for the new structure N-(2-hydroxy-5-nitrosophenyl)acetamide, additionally by 1D- and 2D-NMR. Incubation of microorganisms, such as the soil bacteria Pseudomonas laurentiana, Arthrobacter MPI763, the yeast Papiliotrema baii and Pantoea ananatis, and the plants Brassica oleracea var. gongylodes L. (kohlrabi) and Arabidopsis thaliana Col-0, with N-(2-hydroxy-5-nitrophenyl) acetamide, led to its glucoside derivative as a prominent detoxification product; in the case of Pantoea ananatis, this was together with the corresponding glucoside succinic acid ester. In contrast, Actinomucor elegans consortium synthesized 2-acetamido-4-nitrophenyl sulfate. 1 mM bioactive N-(2-hydroxy-5-nitrophenyl) acetamide elicits alterations in the Arabidopsis thaliana expression profile of several genes. The most responsive upregulated gene was pathogen-inducible terpene synthase TPS04. The bioactivity of the compound is rapidly annihilated by glucosylation.

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Section: Resultsmentioning

confidence: 99%

Bioactive Nitrosylated and Nitrated N-(2-hydroxyphenyl)acetamides and Derived Oligomers: An Alternative Pathway to 2-Amidophenol-Derived Phytotoxic Metabolites

et al. 2022

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“…Since bacterial efflux pumps play a crucial role in the development of AMR both in animals and plants, the identification of efflux pump inhibitors (EPIs) and their coadministration with antibiotic agents has the potential to restore the antibacterial activity of drugs − or agrochemicals − to their original levels. In this scenario, Staphylococcus aureus ( S. aureus ) stands out as the most dangerous superbug among Gram-positive organisms, − with the transmembrane protein NorA being involved in resistance mechanisms against a plethora of structurally unrelated natural and synthetic compounds ( e.g ., quaternary ammonium compounds and antiseptics, phenothiazines and thioxanthenes, totarol, ferruginol, carnosic acid, ethidium bromide (EtBr), tetraphenylphosphonium, rhodamine, acridine, and biocides). , In particular, NorA overexpression is associated with quinolone and fluoroquinolone ( e.g ., ciprofloxacin) resistance …”

Section: Introductionmentioning

confidence: 99%

Fighting Antimicrobial Resistance: Insights on How the Staphylococcus aureus NorA Efflux Pump Recognizes 2-Phenylquinoline Inhibitors by Supervised Molecular Dynamics (SuMD) and Molecular Docking Simulations

Palazzotti

Felicetti

Sabatini

et al. 2023

J. Chem. Inf. Model.

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The superbug Staphylococcus aureus (S. aureus) exhibits several resistance mechanisms, including efflux pumps, that strongly contribute to antimicrobial resistance. In particular, the NorA efflux pump activity is associated with S. aureus resistance to fluoroquinolone antibiotics (e.g., ciprofloxacin) by promoting their active extrusion from cells. Thus, since efflux pump inhibitors (EPIs) are able to increase antibiotic concentrations in bacteria as well as restore their susceptibility to these agents, they represent a promising strategy to counteract bacterial resistance. Additionally, the very recent release of two NorA efflux pump cryo-electron microscopy (cryo-EM) structures in complex with synthetic antigen-binding fragments (Fabs) represents a real breakthrough in the study of S. aureus antibiotic resistance. In this scenario, supervised molecular dynamics (SuMD) and molecular docking experiments were combined to investigate for the first time the molecular mechanisms driving the interaction between NorA and efflux pump inhibitors (EPIs), with the ultimate goal of elucidating how the NorA efflux pump recognizes its inhibitors. The findings provide insights into the dynamic NorA-EPI intermolecular interactions and lay the groundwork for future drug discovery efforts aimed at the identification of novel molecules to fight antimicrobial resistance.

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“…The response system related to this adaptation is based on general stress responses characterized by the bacterial cell's ability to defend itself not only from the specific stress-inducing factors but also from other seemingly unrelated factors [22]. The formation of biofilms to protect cells against the herbicides Aminol, Atectra, Boral, and Heat [23]; cross-resistance by efflux pumps that export glyphosate and antibiotics [24]; increased gene expression and copy number of genes to tolerate pesticide stress [25]; horizontal transfer of genes that confer multi-drug resistant phenotypes [26], and the antioxidant system are examples of general stress response mechanisms. Mutants with deletions in genes encoding Mn-SOD (sodA) and Fe-SOD (sodB) of E. coli K-12, a non-environmental strain not previously exposed to any pesticide, showed tolerance to the Gramoxone herbicide through the activities of isoforms of antioxidant enzymes CAT and SOD [27].…”

Section: Introductionmentioning

confidence: 99%

Bacterial adaptation to rhizosphere soil is independent of the selective pressure exerted by the herbicide saflufenacil, through the modulation of catalase and glutathione S-transferase

Silva,

Flávia da Silva Rovida,

Gabriele Martins

et al. 2023

PLoS ONE

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Herbicides cause oxidative stress in nontarget microorganisms, which may exhibit adaptive responses to substances they have not previously encountered. Nevertheless, it is unclear whether these characteristics occur in bacteria isolated from agricultural soil. Two possible adaptation strategies of Stenotrophomonas sp. CMA26 was evaluated in agricultural soil in Brazil, which is considered stressful due to the intense use of pesticides. The study focused on degradation and antioxidant enzymes in response to the herbicide Heat, which was absent at the isolation site. The results indicated that higher concentrations of herbicide led to more intense stress conditions during the initial periods of growth. This was evidenced by elevated levels of malondialdehyde and peroxide, as well as a significant reduction in growth. Our data show that herbicide degradation is a selection-dependent process, as none of the 35 isolates from the same environment in our collection were able to degrade the herbicide. The stress was controlled by changes in the enzymatic modulation of catalase activity in response to peroxide and glutathione S-transferase activity in response to malondialdehyde, especially at higher herbicide concentrations. This modulation pattern is related to the bacterial growth phases and herbicide concentration, with a specific recovery response observed during the mid phase for higher herbicide concentrations. The metabolic systems that contributed to tolerance did not depend on the specific prior selection of saflufenacil. Instead, they were related to general stress responses, regardless of the stress-generating substance. This system may have evolved in response to reactive oxygen species, regardless of the substance that caused oxidative stress, by modulating of the activities of various antioxidant enzymes. Bacterial communities possessing these plastic tolerance mechanisms can survive without necessarily degrading herbicides. However, their presence can lead to changes in biodiversity, compromise the functionality of agricultural soils, and contribute to environmental contamination through drift.

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A Glyphosate-Based Herbicide Cross-Selects for Antibiotic Resistance Genes in Bacterioplankton Communities

Cited by 16 publications

References 50 publications

Bioactive Nitrosylated and Nitrated N-(2-hydroxyphenyl)acetamides and Derived Oligomers: An Alternative Pathway to 2-Amidophenol-Derived Phytotoxic Metabolites

Bioactive Nitrosylated and Nitrated N-(2-hydroxyphenyl)acetamides and Derived Oligomers: An Alternative Pathway to 2-Amidophenol-Derived Phytotoxic Metabolites

Fighting Antimicrobial Resistance: Insights on How the Staphylococcus aureus NorA Efflux Pump Recognizes 2-Phenylquinoline Inhibitors by Supervised Molecular Dynamics (SuMD) and Molecular Docking Simulations

Bacterial adaptation to rhizosphere soil is independent of the selective pressure exerted by the herbicide saflufenacil, through the modulation of catalase and glutathione S-transferase

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