A General Rate Model of Ion-Exchange Chromatography for Investigating Ion-Exchange Behavior and Scale-up

Mehay, Aaron; Gu, Tingyue

doi:10.4172/1948-5948.1000147

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…The following equation is introduced for the general mass balance equation for describing the behavior of the exchanged counter-ion in the bulk liquid phase, i.e. each ionic species in the liquid phase enters into the ion-exchange column (Mehay & Gu, 2014).…”

Section: General Mass Balance Equation For Iexmentioning

confidence: 99%

Two-Dimensional Anion Exchange coupling Size Exclusion Chromatography Combined with Mathematical Modeling for Downstream Processing of Foot and Mouth Diseases Vaccine

Ghassempour¹,

Hossienizadeh²,

Bagheri³

et al. 2020

Preprint

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The production of purified virus particles with high quality and quantity for vaccine preparation requires scalable purification procedure in downstream step. A purification scheme based on combined strong anion-exchange and size exclusion chromatography (2D -AEC×SEC) is developed for production non-structural protein (NSP) free foot and mouth diseases (FMD) vaccine and the whole procedure is accomplished with 78 % recovery, 85 % virus yield and more than 90 % of residual DNA (rDNA) is removed from the purified vaccine. Due to use AEC as the first column, the injection volume increases four times compare to previous report. Alternatively, a mathematical modeling and simulation approach based on plate model chromatography are developed and matched with the experimental chromatography data to obtain better perception in predicting retention behavior and saving time in downstream scale-up method development. The analysis of purified virus particles by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), dynamic light scattering (DLS) high performance size exclusion chromatography (HP-SEC), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), transmission electron microscopy (TEM) and biological test provide to the best quality of purified FMD virus.

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Section: General Mass Balance Equation For Iexmentioning

confidence: 99%

Two-Dimensional Anion Exchange coupling Size Exclusion Chromatography Combined with Mathematical Modeling for Downstream Processing of Foot and Mouth Diseases Vaccine

Ghassempour¹,

Hossienizadeh²,

Bagheri³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Besides, custom‐designed tools for modeling also commercially available solutions exist, making in silico tools accessible for a broader public. Such programs include Aspen Chromatography (AspenTech) , CADET (Forschungszentrum Jülich) , Chromulator (Ohio University) , ChromWorks (Ypso‐Facto) , and ChromX (Karlsruhe Institute of Technology) .…”

Section: Modeling Approachesmentioning

confidence: 99%

Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches

Baumann

Hubbuch

2016

Engineering in Life Sciences

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The biopharmaceutical industry is at a turning point moving toward a more customized and patient-oriented medicine (precision medicine). Straightforward routines such as the antibody platform process are extended to production processes for a new portfolio of molecules. As a consequence, individual and tailored productions require generic approaches for a fast and dedicated purification process development. In this article, different effective strategies in biopharmaceutical purification process development are reviewed that can analogously be used for the new generation of antibodies. Conventional approaches based on heuristics and high-throughput process development are discussed and compared to modern technologies such as multivariate calibration and mechanistic modeling tools. Such approaches constitute a good foundation for fast and effective process development for new products and processes, but their full potential becomes obvious in a correlated combination. Thus, different combinatorial approaches are presented, which might become future directions in the biopharmaceutical industry.

show abstract

A General Rate Model of Ion-Exchange Chromatography for Investigating Ion-Exchange Behavior and Scale-up

Cited by 2 publications

References 17 publications

Two-Dimensional Anion Exchange coupling Size Exclusion Chromatography Combined with Mathematical Modeling for Downstream Processing of Foot and Mouth Diseases Vaccine

Two-Dimensional Anion Exchange coupling Size Exclusion Chromatography Combined with Mathematical Modeling for Downstream Processing of Foot and Mouth Diseases Vaccine

Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches

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