A General Rate Model of Ion-Exchange Chromatography for Investigating Ion-Exchange Behavior and Scale-up

Abstract

This work presents a general rate model for ion-exchange in column chromatography with the steric massaction isotherm that is suitable for ion-exchange systems involving macromolecules such as large proteins. This comprehensive model considers axial dispersion, interfacial film mass transfer between the bulk-fluid phase and the particle phase, and intraparticle diffusion. The model system has been solved numerically using the finite element method and the orthogonal collocation method with a graphical user interface for Windows based personal computers. The effect of pH is modeled by including buffers, acids, and bases as species in the model system, allowing the induced pH gradients resulted from H + or OH -sorption by the ion exchanger to be described in the model. Feed profiles of all the species can be specified individually allowing complicated gradient patterns. The software, known as Chromulator-IEX, may be a useful tool for the investigation of ion-exchange chromatography behavior and its scale-up.

Two-Dimensional Anion Exchange coupling Size Exclusion Chromatography Combined with Mathematical Modeling for Downstream Processing of Foot and Mouth Diseases Vaccine

“…The following equation is introduced for the general mass balance equation for describing the behavior of the exchanged counter-ion in the bulk liquid phase, i.e. each ionic species in the liquid phase enters into the ion-exchange column (Mehay & Gu, 2014).…”

Two-Dimensional Anion Exchange coupling Size Exclusion Chromatography Combined with Mathematical Modeling for Downstream Processing of Foot and Mouth Diseases Vaccine

“…The following equation is introduced for the general mass balance equation for describing the behavior of the exchanged counter-ion in the bulk liquid phase, i.e. each ionic species in the liquid phase enters into the ion-exchange column (Mehay & Gu, 2014).…”

Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches

“…Besides, custom‐designed tools for modeling also commercially available solutions exist, making in silico tools accessible for a broader public. Such programs include Aspen Chromatography (AspenTech) , CADET (Forschungszentrum Jülich) , Chromulator (Ohio University) , ChromWorks (Ypso‐Facto) , and ChromX (Karlsruhe Institute of Technology) .…”

Two-Dimensional Anion Exchange coupling Size Exclusion Chromatography Combined with Mathematical Modeling for Downstream Processing of Foot and Mouth Diseases Vaccine

“…The following equation is introduced for the general mass balance equation for describing the behavior of the exchanged counter-ion in the bulk liquid phase, i.e. each ionic species in the liquid phase enters into the ion-exchange column (Mehay & Gu, 2014).…”

Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches

“…Besides, custom‐designed tools for modeling also commercially available solutions exist, making in silico tools accessible for a broader public. Such programs include Aspen Chromatography (AspenTech) , CADET (Forschungszentrum Jülich) , Chromulator (Ohio University) , ChromWorks (Ypso‐Facto) , and ChromX (Karlsruhe Institute of Technology) .…”

Two-Dimensional Anion Exchange coupling Size Exclusion Chromatography Combined with Mathematical Modeling for Downstream Processing of Foot and Mouth Diseases Vaccine

“…The following equation is introduced for the general mass balance equation for describing the behavior of the exchanged counter-ion in the bulk liquid phase, i.e. each ionic species in the liquid phase enters into the ion-exchange column (Mehay & Gu, 2014).…”

Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches

“…Besides, custom‐designed tools for modeling also commercially available solutions exist, making in silico tools accessible for a broader public. Such programs include Aspen Chromatography (AspenTech) , CADET (Forschungszentrum Jülich) , Chromulator (Ohio University) , ChromWorks (Ypso‐Facto) , and ChromX (Karlsruhe Institute of Technology) .…”