Pengaruh Metode Ekstraksi Terhadap Kandungan Senyawa Polifenol dan Aktivitas Antioksidan pada Rubus fraxinifolius

Abstract

Rubus fraxinifolius can be founded in the mountainous regions of West Java. Previous studies have shown that this plant has potent antioxidant activity. The content of polyphenol compounds in this species contributes substantially to antioxidant activity, and the extraction method will aff ect the content of the compound. In this study, an evaluation of the extraction method eff ect on leaves, young fruits, and stems of R. fraxinifolius on the content of polyphenols and their antioxidant activity, have been done. The leaves, fruits, and stems of R. fraxinifolius were extracted by maceration, refl ux, and Soxhlet using methanol as a solvent. Each extract was determined the total polyphenol content using Folin-Ciocalteu reagent and antioxidant activity test using DPPH reduction method. The highest extract yield was R. fraxinifolius leaf refl ux extract (30.20%). The highest total polyphenol content was given by the Soxhlet extract of leaf R. fraxinifolius (48.79 mg GAE/g extract). The highest antioxidant activity was leaf Soxhlet extract of R. fraxinifolius (98.29% at 100 ppm). From this study it was shown that the methanol extract of R. fraxinifolius leaves extracted by soxhlet gave the highest polyphenol content and antioxidant activity.Abstrak: Tanaman Rubus fraxinifolius dapat ditemukan di daerah pegunungan Jawa Barat. Penelitian sebelumnya menunjukkan bahwa tanaman ini memiliki aktivitas antioksidan yang sangat kuat. Kandungan senyawa polifenol pada tanaman berkontribusi secara substansial terhadap aktivitas antioksidan dan metode ekstraksi akan mempengaruhi kandungan senyawa yang tersari. Pada penelitian ini dilakukan evaluasi pengaruh metode ekstraksi organ daun, buah muda, dan batang R. fraxinifolius terhadap kandungan polifenol serta aktivitas antioksidannya. Bagian daun, buah, dan batang R. fraxinifolius diekstraksi cair padat secara maserasi, refl uks, dan Soxhlet menggunakan pelarut metanol. Terhadap masing-masing ekstrak dilakukan penetapan kadar polifenol total dengan pereaksi Folin-Ciocalteu serta uji aktivitas antioksidan dengan metode peredaman DPPH. Rendemen ekstrak terbanyak adalah ekstrak refl uks daun R. fraxinifolius sebesar 30,20%. Kandungan polifenol total tertinggi adalah pada ekstrak Soxhlet daun R. fraxinifolius sebesar 48,79 mg GAE/g ekstrak. Aktivitas antioksidan tertinggi yaitu ekstrak Soxhlet daun R. fraxinifolius sebesar 98,29% pada 100 bpj. Dari penelitian ini menunjukan bahwa ekstrak metanol daun R. fraxinifolius yang diekstraksi secara Soxhlet memiliki kandungan polifenol serta aktivitas antioksidan tertinggi.Kata kunci: Rubus fraxinifolius, metode ekstraksi, rendemen, polifenolat, antioksidan.

The effect of extraction methods towards antioxidant activity of ethanol extract of Picria fel-terrae Lour. Herbs

“…The antioxidant testing method using the DPPH method is the method that gives the best results because the DPPH method has more stable radical properties than the elements of other antioxidant methods, making it easier to convert free radicals into non-radical compounds. The extraction method using the hot soxhlet method is an iterative extraction method that can be more certain that the extraction process is perfect and the metabolite compounds are more completely attracted by the occurrence of a colour change in the extraction process from coloured to colourless, which ensures that the extraction process has been completed [16,26] Table 1 shows the results of antioxidant tests using Picria fel-terrae Lour. Herbs ethanol extract with IC 50 values.…”

“…Using an extraction method with a ratio of time and solvent requirements greatly determines the efficiency of extraction, in addition to the use of temperature, which has a significant impact on the quality (biological activity in vitro or in vivo) and quantity (amount or yield) of the extract obtained [14,15]. Cold extraction (maceration and percolation) and hot extraction (reflux and soxhletation) are the most popular extraction processes [16]. Based on this background, we want to see how different extraction procedures (maceration, percolation, and reflux) affect antioxidant activity, total phenolic content, and total flavonoid content when using ethanol as a solvent.…”

Pengaruh Metode Ekstraksi Terhadap Kandungan Fenolik Total dan Flavonoid Total Pada Ekstrak Etanol Buncis (Phaseolus vulgaris L.)

“…Mulai dari teknik konvensional seperti maserasi, sokletasi, dan refluks, hingga teknik yang lebih maju seperti ekstraksi gelombang mikro, ekstraksi ultrasonik, dan ekstraksi fluida superkritis [6]. Untuk memperoleh ekstrak dengan aktivitas biologi tertinggi serta dalam pengembangan obat herbal maka perlu dilakukan observasi metode ekstraksi [7].…”