Genetic variability within Septoria carvi Syd.- a pathogen of caraway Carum carvi L

Abstract

Genetic variability within Septoria carvi isolates obtained from various organs of caraway cultivated in south-eastern and central Poland was studied using the RAPD-PCR technique. The tests were performed using randomly selected primers. The DNA profiles obtained using four primers proved useful in determining genetic variability among the genotypes of Septoria carvi isolates. The present study characterized the differences in the nucleotide sequence within the internal transcribed spacer region of rDNA (ITS1, 5.8S, ITS2) of selected S. carvi isolates and reference strains of Septoria spp. Moreover, eight isolates were sequenced for three loci: actin, calmodulin and translation elongation factor 1-alpha, and the obtained sequences were compared with the sequences of Septoria reference strains affecting other plants of the family Apiaceae. Phylogenetic analysis showed distinct differences of the tested isolates, which allowed to treat them Septoria carvi species affecting the above-ground organs of caraway Carum carvi L. This study is the first report on the genetic characteristics of the species S. carvi.

Characterization and pathogenicity of Septoria gaurina associated with leaf blotch disease on Gaura parviflora in China

“…A multilocus polyphasic sequencing approach based on ITS, LSU , and Tub2 sequences was first applied to identify Septoria spp. by Feau et al (2006), which separated closely related species into distinct monophyletic groups (Crous et al, 2001; Quaedvlieg et al, 2013; Zalewska et al, 2017). Therefore, in this study, we used morphological characteristics combined with phylogenetic analysis to confirm that the causal agent of leaf blotch on G .…”